Behavior Core

行为核心

• 批准号: 7905913
• 负责人: William A. Carlezon
• 金额: $ 32.03万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7905913
• 关键词: Acute; Affective; Animal Model; Animals; Antidepressive Agents; Anxiety; Appetitive Behavior; Behavior; Behavioral; Biological Assay; Blood; Brain; Chronic; Cognitive; Complex; Corticosterone; Data; Ensure; Food; Gene Transfer; Genes; Goals; Human Resources; Incentives; Individual; Learned Helplessness; Measurement; Measures; Molecular Target; Moods; Motivation; Motor Activity; Mus; Mutagenesis; Names; Neurosecretory Systems; Plasma; Proteins; Rattus; Research Personnel; Rewards; Role; Self Stimulation; Sex Behavior; Social Interaction; Stress; Swimming; Testing; Transgenic Organisms; Viral Genes; Work; behavior test; depression; mood regulation; neuroadaptation; pre-clinical; relating to nervous system; research study; response; social

Center investigators are studying numerous genes and their protein products in the brain's reward circuits to define their role in the regulation of mood and motivation under normal circumstances and in animal models of depression and antidepressant action. To accomplish this goal, the Behavioral Core has established an extremely broad battery of behavioral tests in rats and mice. This battery includes routine measures of locomotor activity and anxiety-like behavior,, as well as several standard depression-related tests such as the forced swim and learned helplessness tests. The battery also incorporates several additional assays that provide complementary information about an animal's affective state; these include measures of sexual behavior, incentive motivation for food, intracranial self-stimulation, social interaction, and sophisticated cognitive tasks, to name some examples. In addition, Core personnel continually work to extend this battery to additional tests. For example, we recently adapted the social defeat paradigm, where certain behavioral abnormalities respond to chronic (but not acute) antidepressant treatment, to study stress-induced neuroadaptations in mouse brain. The imperative to employ such a large battery of behavioral tests is that it is difficult to infer something about complex behavior from a single test or even a limited number of tests. Rather, by utilizing numerous complementary measures we are able to infer, with much greater accuracy, the role of a given gene in complex behavior related to depression. By consolidating these behavioral tests within a centralized Core, we can ensure rigorous control over the data as well as facilitate comparisons and contrasts of experimental results from the individual Projects. This consolidation also makes financial sense, since we can concentrate and maximize efficient use of our behavioral expertise. The role of specific molecular targets in behavioral responses related to mood and motivation are tested with a variety of approaches, including advanced mouse mutagenesis and viral gene transfer in conjunction with the Transgenic Core. The Behavioral Core then provides routine, relatively high throughput behavioral tests for investigators in the Center's preclinical Projects (1-4). Encouraging findings are pursued with more sophisticated behavioral tests also via this Core. In addition, the Core obtains routine neuroendocrine measurements (e.g., plasma corticosterone levels) and antidepressant blood levels in behaving animals as needed for particular experiments.

中心研究人员正在研究大脑奖励电路中的许多基因及其蛋白质产品 在正常情况下和动物模型中定义他们在调节情绪和动机的调节中的作用 抑郁和抗抑郁作用的作用。为了实现这一目标，行为核心已经建立了一个 大鼠和小鼠的行为测试极为广泛。该电池包括常规措施 运动活动和类似焦虑的行为，以及几种与标准抑郁症相关的测试，例如 强迫游泳和学习的无助测试。电池还包含了其他几种测定 提供有关动物情感状态的互补信息；这些包括性措施 行为，食物的激励动机，颅内自我刺激，社交互动和复杂的 认知任务，列举一些示例。此外，核心人员不断努力扩展该电池 进行其他测试。例如，我们最近改编了社交失败范式，某些行为 异常对慢性（但没有急性）抗抑郁治疗的反应，以研究压力诱导 小鼠大脑中的神经适应。必须采用如此大的行为测试的必要条件是 很难从单个测试甚至有限数量的测试中推断出有关复杂行为的信息。 相反，通过利用许多互补措施，我们能够以更高的准确性来推断 给定基因在与抑郁有关的复杂行为中的作用。通过巩固这些行为测试 在集中核心内，我们可以确保对数据进行严格控制，并促进比较和 各个项目的实验结果的对比。这种合并也很有意义， 由于我们可以集中并最大程度地利用我们的行为专业知识。 特定分子靶标在与情绪和动机有关的行为反应中的作用。 各种方法，包括高级小鼠诱变和病毒基因转移以及与 转基因核心。然后，行为核心提供常规，相对较高的吞吐量行为测试 适用于中心临床前项目的调查人员（1-4）。令人鼓舞的发现被更多 还通过此核心进行了复杂的行为测试。此外，核心获得常规神经内分泌 在行为动物中的测量（例如血浆皮质酮水平）和抗抑郁药水平 需要特定实验。