CORE--Bioanalytical

核心--生物分析

• 批准号: 7786061
• 负责人: Bruce Alan Freeman
• 金额: $ 55.89万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-01-15 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7786061
• 关键词: Adopted; Animal Model; Animals; Biocompatible Materials; Biology; Blood; Blood Vessels; Cardiac; Cardiovascular system; Cells; Chemistry; Clinical; Clinical Protocols; Congestive Heart Failure; Data; Data Quality; Development; Disease; Dissection; Ensure; Faculty; Fatty Acids; Free Radicals; Functional disorder; Future; Gene Expression; Generations; Goals; Individual; Inflammatory; Literature; Measurement; Measures; Methodology; Microscopic; Molecular; Nitrates; Outcome; Oxidation-Reduction; Participant; Procedures; Process; Production; Proteins; Proteomics; Quality Control; Resolution; Resources; Sampling; Services; Signal Pathway; Signal Transduction; Standardization; Techniques; Testing; Therapeutic; Tissue Sample; Tissues; Ursidae Family; base; design; indexing; insight; interest; laser capture microdissection; meetings; novel; response; success

The purpose of Bioanalytical services are to a) facilitate experimental aims being pursued in the context of both animal studies and clinical protocols, b) integrate key experimental goals c) provide consistent and responsive high quality analytical data that supports the hypothesis-testing and d) develop and apply new analytical methodologies that will meet the evolving needs and successes. The Bioanalytical faculty participants and their staff are established experts in inflammatory and vascular biology, cell signaling, proteomics, lipidomics and redox chemistry. They will bring to bear their individual expertise in collaborative and service functions designed to support the proposed goals of this SCCOR. Specific Bioanalytical objectives include a) providing sensitive and unambiguous measurement of indices of vascular and cardiac tissue inflammatory processes and their resolution, b) optimization and standardization of microscopic-, immunological-, mass spectrometric- and PCR-based techniques for the tissue localization, quantification and/or characterization of specific mRNAs and proteins, c) the application of sensitive and specific mass spectrometric approaches for the measurement of products of cardiovascular inflammatory signaling and their sequelae - specifically, oxidized fatty acids, and oxidized and nitrated proteins and d) the generation of new insight regarding inflammatory mechanisms and target molecules that contribute to CHF, that will in turn motivate future novel hypothesis-driven experimental and therapeutic approaches. In the integrated clinical and basic investigatory approach adopted by this SCCOR proposal, Bioanalytical will thus provide a consistent resource for measurement of key experimental endpoints serving as outcome variables related to progression and resolution of CHF. Use will ensure uniformity in a) blood, microdialysate and tissue sampling, b) biomaterial processing and c) sample storage between projects. We will also contribute to the design of quality control measures underlying procedures executed and the overall design and planning of experimental aims.

生物分析服务的目的是a）促进实验目标 动物研究和临床方案的背景，b）整合关键实验目标c）提供一致且响应的高质量分析数据，以支持假设检验和d）开发和应用新的分析方法，以满足不断发展的需求和成功。生物分析的教师及其工作人员是炎症和血管生物学，细胞信号，蛋白质组学， 脂质组学和氧化还原化学。他们将带来旨在支持SCCOR拟议目标的协作和服务功能方面的个人专业知识。特定的生物分析目标包括a）提供敏感和明确的测量 血管和心脏组织炎症过程的指标及其分辨率，b）基于显微镜，免疫学，质谱和PCR的优化和标准化，用于组织定位，定量和/或表征特定mRNA和蛋白质的量化和/或表征敏感性和特定质量光谱仪的应用 - 具体而言，氧化脂肪酸，氧化和硝化蛋白质以及d）关于炎症机制和靶向分子的新见解的产生，这反过来又可以激发新的新型假设驱动的实验和治疗方法。在此SCCOR建议采用的综合临床和基本研究方法中，生物分析将为测量与CHF的进展和分辨率有关的结果变量的关键实验终点提供一致的资源。 使用将确保在a）血液，微疗法和组织采样中均匀性，b） 生物材料处理和c）项目之间的样本存储。我们还将为 质量控制措施的设计基础程序和整体的设计 实验目标的设计和计划。