Mapping the Interactions and Dynamics that Organize Bacteria Cells

绘制细菌细胞组织的相互作用和动态图

• 批准号: 10341319
• 负责人: Julie Biteen
• 金额: $ 31.73万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-01 至 2026-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10341319
• 关键词: Address; Bacteria; Bacterial Model; Bacteriology; Biochemical Reaction; Biochemistry; Biology; Biophysics; Caulobacter crescentus; Cell physiology; Cells; Cellular biology; Color; Communities; Cyanobacterium; DNA; Data Set; Detection; Diffusion; Disease; Escherichia coli; Eukaryota; Fluorescence; Goals; Health; Human; Image; Imaging Device; In Vitro; Maps; Measurement; Measures; Methods; Microscopy; Modeling; Molecular; Motion; Outcome; Phase; Physical condensation; Positioning Attribute; Prokaryotic Cells; Proteins; Resolution; Ribonucleoproteins; Role; Rotation; Structure; System; Testing; Time; Tube; Work; cell type; commensal bacteria; data quality; density; fighting; human disease; improved; in vitro Model; innovation; microbial; molecular scale; nanoscale; next generation; novel; novel strategies; organizational structure; scaffold; single molecule; spatiotemporal; statistics; tool

Project Summary Molecular-scale interactions enable subcellular organization, but the current state-of-the-art does not include a generalizable method for measuring how molecules move and cooperate on the nanometer scale and in real time within the cell. This gap is particularly striking in bacteria cells. Accordingly, our picture of the organization of the bacterial cell is incomplete. This proposal aims to understand how cellular components organize—by scaffolding, aggregation, phase separation, or otherwise—to produce a general model of bacterial cell organization and ultimately enable us to promote commensal bacteria or fight human disease. Thus, we aim to measure the positioning, interactions, and motions of molecules in living bacterial cells in different protein systems implicated in the sub-cellular organization of these cells. This proposal will develop these next- generation super-resolution tools through three synergistic specific aims: (1) to super-resolve how single- molecule dynamics vary in space and in time in living bacterial cells; (2) to super-resolve sub-cellular interactions in space and time in living bacteria cells; and (3) to improve the detection of single molecules in living bacteria cells. The toolkit that we develop to map molecular motions and interactions will be broadly applicable to the single-molecule bacteriology community. Furthermore, by focusing on applications in bacterial cell biology, the tools developed in this project will have a widespread, positive biomedical impact by making accessible long- term, significant questions in microbial biology.

项目概要 分子尺度的相互作用使亚细胞组织成为可能，但目前最先进的技术不包括 用于测量分子如何在纳米尺度上真实移动和协作的通用方法 细胞内的时间差距在细菌细胞中尤其明显。 该提案旨在了解细胞成分如何组织。 支架、聚集、相分离或其他方式——产生细菌细胞的通用模型 组织并最终使我们能够促进共生细菌或对抗人类疾病。 测量活细菌细胞中不同蛋白质分子的定位、相互作用和运动 该提案将开发这些细胞的亚细胞组织相关的系统。 通过三个协同的具体目标生成超分辨率工具：（1）超分辨率如何单 (2) 超分辨亚细胞相互作用 （3）改善活细菌中单分子的检测； 我们开发的用于绘制分子运动和相互作用的工具包将广泛适用于细胞。 此外，通过专注于细菌细胞生物学的应用， 该项目开发的工具将通过提供长期可用的工具来产生广泛的、积极的生物医学影响。 术语，微生物生物学中的重要问题。