Flow Cytometry Shared Resources

流式细胞术共享资源

• 批准号: 10443637
• 负责人: Huw S. Kruger Gray
• 金额: $ 21.94万
• 依托单位: UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-07-10 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10443637
• 关键词: Activation Analysis; Apoptosis; Applications Grants; Bioinformatics Shared Resource; Biological; Biological Assay; Biometry; Cancer Center; Cancer Center Support Grant; Cell Cycle; Cell Separation; Cell surface; Cells; Clone Cells; Cloning; Complex; Comprehensive Cancer Center; Consultations; Cytometry; Data Analyses; Epitopes; Experimental Designs; Facility Accesses; Flow Cytometry; Flow Cytometry Shared Resource; Fluorescence; Fluorescence-Activated Cell Sorting; Funding; Hour; Immunophenotyping; Knowledge; Label; Lasers; Measurement; Methods; Normal Cell; Peer Review; Phenotype; Ploidies; Population; Preparation; Property; Publications; Quality Control; Reagent; Research; Research Personnel; Research Project Grants; Research Support; Research Training; Reservations; Resource Sharing; Sampling; Services; Side; Signal Transduction; Speed; Suspensions; System; Technical Expertise; Technology; Training; Tube; Work; antigen detection; base; cancer cell; cancer genomics; cell analyzer; data acquisition; design; experience; experimental analysis; experimental study; innovation; instrument; instrumentation; light scattering; member; novel; operation; peer support; programs; single cell analysis

PROJECT SUMMARY: FLOW CYTOMETRY SHARED RESOURCE The Flow Cytometry Shared Resource (FCSR) at Sylvester Comprehensive Cancer Center (Sylvester) is a state-of-the-art facility offering comprehensive analytical flow cytometry and high-speed cell sorting services that are pivotal to the successful progress of many research projects. The FCSR Director and staff work collectively to provide professional consultation, assay design, and research training as well as full cytometric analysis and cell sorting services to support research programs, publications, and grant applications. The FCSR’s six sophisticated fluorescence flow cytometry instruments (three cell analyzers and three cell sorters) facilitate a wide range of complex experiments examining the biological properties of both normal and cancer cells. The FCSR has the capability to provide simultaneous analysis of up to 19 different fluorescent parameters and two laser light-scatter parameters (forward and side scatter) for both cell surface and intracellular antigen detection in complex cell mixtures. The FCSR performs a variety of experimental analyses, such as complex immunophenotyping, DNA content and cell cycle analysis, cell signaling and activation analysis, and measurement of proliferation and apoptosis. Further, the FCSR provides fluorescence-activated cell sorting services, which allow investigators to purify both bulk and single cells at high purity and high speed, based upon complex multiparametric phenotyping. The three cell sorters have the potential to flow-sort up to 17 fluorescence parameters into bulk tubes or into multi-well plates for single cell flow cloning under Biosafety Level 2 conditions. The FCSR’s three highly experienced technicians perform cell sorting as well as continuous quality control assessment for all instruments. They also provide comprehensive user training in all aspects of flow cytometric experiments, including experimental design, fluorescent reagent panel selection, instrument setup and operation, data acquisition, and data analysis. Users are trained such that they are capable of operating the analysis cytometers themselves and then are permitted to do so independently during or outside regular operating hours. Users access the facility through an online instrument reservation and invoicing system. In 2017, the FCSR provided cytometry services to 41 Sylvester members (95% of whom were supported by peer-reviewed funding), which comprised 78% of total FCSR usage.

项目摘要：流式细胞术共享资源 西尔维斯特综合癌症中心（西尔维斯特）的流式细胞术共享资源 (FCSR) 是一个 最先进的设施提供全面的分析流式细胞术和高速细胞分选服务 这对于许多研究项目的成功进展至关重要。 共同提供专业咨询、检测设计、研究培训以及完整的细胞分析 分析和细胞分选服务，以支持研究计划、出版物和拨款申请。 FCSR 的六台精密荧光流式细胞仪（三台细胞分析仪和三台细胞分选仪） 促进广泛的复杂实验，检查正常和癌症的生物学特性 FCSR 能够同时分析多达 19 种不同的荧光细胞。 参数和两个激光光散射参数（前向和侧向散射）用于细胞表面和 FCSR 执行各种实验分析，复杂细胞混合物中的细胞内抗原检测。 例如复杂的免疫表型分析、DNA 含量和细胞周期分析、细胞信号传导和激活 此外，FCSR 还提供荧光激活功能。 细胞分选服务，使研究人员能够以高纯度和高速纯化散装细胞和单细胞， 基于复杂的多参数表型分析，这三种细胞分选仪具有流式分选的潜力。 将 17 个荧光参数输入到散装管或多孔板中，以便在生物安全下进行单细胞流式克隆 2 级条件 FCSR 的三名经验丰富的技术人员执行细胞分选以及连续操作。 他们还提供所有仪器的质量控制评估。 流式细胞术实验，包括实验设计、荧光试剂组选择、仪器 用户经过培训，能够进行设置和操作、数据采集和数据分析。 自己操作分析细胞仪，然后允许在期间或之外独立进行操作 用户通过在线仪器预订和发票访问该设施。 2017 年，FCSR 为 41 名西尔维斯特会员（其中 95% 为会员）提供了细胞计数服务。 由同行评审资金支持），占 FCSR 使用总量的 78%。