High-resolution bidirectional optical-acoustic mesoscopic neural interface for image-guided neuromodulation in behaving animals

用于行为动物图像引导神经调节的高分辨率双向光声介观神经接口

• 批准号: 10407380
• 负责人: Robert E. Campbell
• 金额: $ 252.56万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-01 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10407380
• 关键词: 3-Dimensional; Acoustic Stimulation; Acoustics; Algorithms; Animals; Area; Behavior; Behavioral; Biliverdin reductase; Biliverdine; Binding; Biosensor; Brain; Cells; Development; Engineering; Fluorescence; Functional Imaging; Generations; Genetic; Goals; Head; Hybrids; Image; Knockout Mice; Label; Lead; Lighting; Link; Measurement; Methods; Microscopic; Modality; Modernization; Mus; Neurons; Odors; Olfactory Pathways; Optics; Organism; Penetration; Performance; Population; Protein Engineering; Protocols documentation; Reporting; Resolution; Signal Transduction; Source; Structure; Surface; System; Techniques; Technology; Testing; Tissue imaging; Tissues; Transducers; Ultrasonic wave; Ultrasonics; Variant; Work; awake; base; behavioral study; chromophore; cofactor; deep learning; design; design and construction; image guided; in vivo; in vivo imaging; millimeter; neuroimaging; neuroregulation; next generation; novel; olfactory bulb; olfactory stimulus; optical imaging; optoacoustic tomography; photoacoustic imaging; reconstruction; relating to nervous system; response; spatiotemporal; success; targeted imaging; tool; ultrasound

SUMMARY Acoustic technologies such as optoacoustic (OA) imaging and ultrasound neuromodulation (USNM) are poised to revolutionize deep tissue, high-resolution, large-scale, in vivo imaging, and neurostimulation in mammalian organisms. These advances are enabled by the high tissue penetrability of ultrasound (US) waves, and present untapped and exciting opportunities for accessing structures throughout the mammalian brain for precise control and measurement of neural activity. We have recently introduced hybrid tools for parallel OA imaging with GCaMP-type indicators and US neuromodulation in the cortex in vivo. Despite these advances, improvements in the spatial resolution, as well as the capability to make full use of the deep-tissue access afforded by these modalities, are highly desirable. We propose to undertake technological developments designed to optimize these methods for cellular resolution, deep-tissue functional imaging, and neurostimulation, through both optimization of hardware and ‘wetware, such as near-infrared (NIR) functional probes. These developments to optimize functional OA signals will enable imaging of neural activity at tissue depths that far exceed those accessible with optical techniques alone. The new framework will then be applied and tested in the mouse olfactory system, whose spatial-temporal scale of neural activity is well matched to that of the proposed technique, and which will significantly benefit from a combined imaging and perturbation strategy for linking large-scale odor-evoked neural activity in the olfactory bulb to mouse behavior.

概括 光声 (OA) 成像和超声神经调节 (USNM) 等声学技术有望彻底改变哺乳动物的深层组织、高分辨率、大规模活体成像和神经刺激，这些进步是由高组织穿透性实现的。超声波（US），并为访问整个哺乳动物大脑的结构以精确控制和测量神经活动提供了尚未开发的令人兴奋的机会，我们最近推出了用于与 GCaMP 型并行 OA 成像的混合工具。尽管取得了这些进展，但空间分辨率的提高以及充分利用这些模式提供的深层组织通路的能力仍然是非常可取的。旨在通过优化硬件和“湿件”（例如近红外（NIR）功能探针）来优化这些方法，以实现细胞分辨率、深层组织功能成像和神经刺激。这些优化功能性 OA 信号的开发将使神经成像成为可能。活动于然后，新框架将在小鼠嗅觉系统中应用和测试，其神经活动的时空尺度与所提出的技术非常匹配，这将大大受益。来自将嗅球中大规模气味诱发的神经活动与小鼠行为联系起来的组合成像和扰动策略。