Behavioral Measures of Temporal Resolution in Mice With or Without Kv1.1 Subunits

有或没有 Kv1.1 亚基的小鼠时间分辨率的行为测量

• 批准号: 7567530
• 负责人: MICHEAL L DENT
• 金额: $ 14.79万
• 依托单位: STATE UNIVERSITY OF NEW YORK AT BUFFALO
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7567530
• 关键词: Affect; Animals; Ataxia; Auditory; Auditory system; Behavior; Behavioral; Behavioral Paradigm; Chronic; Detection; Epilepsy; Genes; Hearing; Human; In Vitro; Knock-out; Knockout Mice; Link; Measures; Methods; Mus; Muscle; Mutation; Names; Physiological; Principal Investigator; Process; Research; Resolution; Sound Localization; Stress; Testing; Voltage-Gated Potassium Channel; Wild Type Mouse; auditory stimulus; awake; behavior measurement; in vivo; programs; research study; response; sound

DESCRIPTION (provided by applicant): The overall objective of the proposed experiments is to examine the behavioral abilities of mice to hear in several listening situations. We will test the detection of simple sounds in quiet, the monaural temporal resolution acuity, and binaural sound localization accuracy. Once reliable behavioral paradigms are established for normal mice, these same measures will be taken on mice lacking the voltage-gated potassium channel subunit Kv1.1. Both in vivo and in vitro physiological studies on these Kcna1 knockout mice suggest that these animals should have normal hearing capabilities for detecting pure tones in quiet, but should have poor temporal resolution. Whether this temporal resolution will manifest itself in both monaural and binaural temporal processing situations is unknown, so both will be measured. Although physiological studies comparing knockouts to wild-type mice are important, they do not tell us how the whole, awake, behaving animal is affected. The experiments proposed here will reveal any type of deficit that may be seen in mice lacking a completely normally-functioning auditory system. These experiments will establish a reliable method for testing auditory behavior in mice that can be used for both normal subjects and for those with abnormal auditory functioning. Mice lacking the gene Kcna1, a mutation linked to epilepsy, stress-induced ataxia, and chronic muscle twitching in humans, show abnormal physiological responses to auditory stimuli. It is unknown, however, how this affects the 'real world' listening situation of these animals, so this will be determined here.

描述（由申请人提供）：拟议实验的总体目标是检查小鼠在几种听力情况下听到的行为能力。我们将在安静，单声道的时间分辨率敏锐度和双耳声音定位精度中测试简单声音的检测。一旦为正常小鼠建立了可靠的行为范例，将对缺乏电压门控钾通道亚基KV1.1采取相同的措施。对这些KCNA1基因敲除小鼠的体内和体外生理研究都表明，这些动物应具有正常的听力能力，以便在安静的情况下检测纯音，但时间分辨率应较差。这种时间分辨率是否会在单脉和双耳的时间处理情况下表现出来，因此尚不清楚。尽管将敲除与野生型小鼠进行比较的生理研究很重要，但它们并没有告诉我们整体，清醒，行为动物如何受到影响。这里提出的实验将揭示缺乏完全正常功能的听觉系统的小鼠中可能看到的任何类型的赤字。这些实验将建立一种可靠的方法来测试小鼠的听觉行为，该方法可用于正常受试者和具有异常听觉功能的人。缺乏基因KCNA1的小鼠是与人类癫痫，压力引起的共济素和长期肌肉抽搐有关的突变，对听觉刺激的生理反应异常。然而，这是未知的，这如何影响这些动物的“现实世界”聆听状况，因此将在这里确定。