Understanding The Impact of Diabetes on Implant Performance: A Retrieval Study

了解糖尿病对植入物性能的影响：一项检索研究

• 批准号: 10397567
• 负责人: Alexandra Arteaga
• 金额: $ 4.29万
• 依托单位: UNIVERSITY OF TEXAS DALLAS
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-05-15 至 2023-05-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10397567
• 关键词: Address; Adhesions; Adverse effects; Affect; Age; Alloys; Area; Biological; Biological Factors; Bone Tissue; Cell Proliferation; Cells; Characteristics; Chemicals; Chromium; Clinical; Cobalt; Corrosion; Deposition; Devices; Diabetes Mellitus; Diet Habits; Dislocations; Environment; Exposure to; Failure; Future; Gender; Glucose; Goals; Habits; Image; Immune response; Impaired wound healing; Implant; Infection; Inflammation; Inflammatory Response; Investigation; Ketones; Lead; Life Style; Metals; Microscopic; Microscopy; Modification; Morphology; Motion; Necrosis; Nutritional; Optics; Orthopedics; Osseointegration; Oxides; Patients; Performance; Physical activity; Population; Preparation; Procedures; Property; Protocols documentation; Publications; Recording of previous events; Retrieval; Roentgen Rays; Sampling; Scanning Electron Microscopy; Series; Smoking; Specimen; Spectrum Analysis; Succinates; Surface; Surface Properties; Surgeon; Techniques; Testing; Time; Tissues; Titanium; Urea; Visual; X ray spectroscopy; base; bone; bone healing; collagenase; combat; design; diabetic; diabetic patient; diet and exercise; drinking; healing; high risk; immunoregulation; implant material; improved; in vitro testing; interest; macrophage; metallicity; non-diabetic; oxidation; response; success

Proposal Summary and Abstract Orthopedic devices have often been considered a higher risk to diabetic patients due to impaired wound healing abilities that can be caused by reduced macrophages, collagenase, and cell proliferation. It is still not well understood exactly which biological factors determine how effective bone healing is surrounding orthopedic implants within diabetic conditions. This can have adverse effects on the success and performance of the implant due to corrosion of the surface, micro motion, dislocation, infection or necrosis of the surrounding tissues, or even surgeon error. In order to better determine how future implants can become more successful in diabetic patients we would like to establish a correlation between the amount of decay of the implant surface and the environment that the implant was exposed to. The overall goal of this study is to understand surface properties, morphological features and failure modes of implants removed from diabetic patients. In summary, the implants received for analysis will be first subjected to optical microscopy to identify the degree of biological and metallic products present on the surface. This technique will also enable identification of areas with interesting features for further analysis. Then the implants will be ranked for the degree of visual biological and metallic/wear deposition. Specimens will be subsequently subjected to a series of cleaning steps following previous protocols developed in our group for surface preparation. Specimens will be again imaged with optical microscopy, areas of interest will be marked and ranked for degree of biological and wear products. Then specimens will be investigated with Scanning Electron Microscopy, followed by Energy Dispersive X-ray Spectroscopy and X-ray Photoelectron Spectroscopy (XPS) for analysis of the chemical composition and oxidation state, respectively, of surfaces. Following this, specimens will be subjected to electrochemical testing for investigation of the corrosion properties of the materials. Immunomodulation may be an important mechanism to improve osseous healing under compromised systemic conditions. Further investigation is warranted of the surface characteristics that can modulate the inflammatory response to promote osseous healing.

提案摘要和摘要 由于伤口受损，骨科器械通常被认为对糖尿病患者来说风险较高 巨噬细胞、胶原酶和细胞增殖减少可能导致愈合能力下降。仍然不是 清楚地了解哪些生物因素决定周围骨骼愈合的有效性 糖尿病患者的骨科植入物。这可能会对成功和绩效产生不利影响 由于表面腐蚀、微动、错位、感染或坏死而导致的植入物损坏 周围组织，甚至是外科医生失误。为了更好地确定未来的植入物如何发展 在糖尿病患者中更成功，我们希望建立衰变量之间的相关性 植入物表面和植入物所暴露的环境。本研究的总体目标是 了解从糖尿病患者体内取出的植入物的表面特性、形态特征和失效模式 患者。 总之，收到用于分析的植入物将首先进行光学显微镜检查以识别 表面上存在的生物和金属产品的程度。该技术还可以实现身份识别 具有有趣特征的区域以供进一步分析。然后将根据视觉程度对植入物进行排名 生物和金属/磨损沉积。样本随后将接受一系列清洁步骤 遵循我们小组之前开发的表面处理协议。标本将再次成像 使用光学显微镜，将标记感兴趣的区域并根据生物和磨损程度进行排名 产品。然后将使用扫描电子显微镜对样本进行研究，然后进行能量研究 用于化学分析的色散 X 射线光谱和 X 射线光电子能谱 (XPS) 分别是表面的成分和氧化态。此后，样本将受到 用于研究材料腐蚀特性的电化学测试。免疫调节可能 是在受损的全身条件下改善骨愈合的重要机制。更远 有必要研究可以调节炎症反应的表面特征 促进骨质愈合。

项目成果

Diabetes as a Risk Factor for Orthopedic Implant Surface Performance: A Retrieval and In Vitro Study.

糖尿病作为骨科植入物表面性能的危险因素：检索和体外研究。

• 发表时间: 2021-06
• 作者: Arteaga, Alexandra;Qu, Jiayi;Haynes, Sara;Webb, Brian G;LaFontaine, Javier;Rodrigues, Danieli C
• 通讯作者: Rodrigues, Danieli C

Exogenous Protein Delivery of Ionic Liquid-Mediated HMGB1 Coating on Titanium Implants.

钛植入物上离子液体介导的 HMGB1 涂层的外源蛋白质递送。

• 发表时间: 2023-02-14
• 作者: Arteaga, Alexandra;Ranathunga, Dineli T S;Qu, Jiayi;Biguetti, Claudia C;Nielsen, Steven O;C Rodrigues, Danieli
• 通讯作者: C Rodrigues, Danieli

Biological Effects of New Titanium Surface Coatings Based on Ionic Liquids and HMGB1: A Cellular and Molecular Characterization in Lewis Rats.

基于离子液体和 HMGB1 的新型钛表面涂层的生物效应：路易斯大鼠的细胞和分子表征。

• DOI: 10.1021/acsbiomaterials.3c00367
• 发表时间: 2023-07-07
• 期刊: ACS biomaterials science & engineering
• 影响因子: 5.8
• 作者: Ale;ra Arteaga;ra;C. Biguetti;Bhuvana Lakkasetter Ch;rashekar;rashekar;Jimena Mora;Adeena Qureshi;D. Rodrigues
• 通讯作者: D. Rodrigues