CORE--CELL /MOLECULAR /MICROSCOPY

核心--细胞/分子/显微镜

• 批准号: 7688517
• 负责人: Gordon Frank Tomaselli
• 金额: $ 48.23万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7688517
• 关键词: Antibodies; Biological; Biology; Biomedical Engineering; Canis familiaris; Cardiology; Cells; Chemicals; Connexins; Core Facility; Cost Savings; Data; Databases; EST Library; Fingerprint; Gene Proteins; Heart; Image; Kinetics; Laboratories; Liquid Chromatography; Location; Mass Spectrum Analysis; Medicine; Messenger RNA; Microarray Analysis; Microscopy; Molecular; Molecular Genetics; Peptides; Performance; Polymerase Chain Reaction; Preparation; Protein Databases; Proteins; Proteomics; Protocols documentation; Qualifying; Resources; Sarcoplasmic Reticulum; Services; Spottings; Technical Expertise; Time; Tissues; Two-Dimensional Gel Electrophoresis; Western Blotting; cancer care; gel electrophoresis; medical schools; protein expression; repository; research study

The molecular genetics and proteomics core serves as a resource to provide expertise and assistance in the analysis of gene and protein expression to projects by Van Eyk and Tomaselli. The emphasis of the core will be on the preparation and utilization of mRNA and protein for the performance and analysis of microarray and protein expression studies. Activities of the core facility will be performed in several locations on the campus of the Johns Hopkins School of Medicine including laboratories in the Division of Cardiology, the Sidney Kimmel Comprehensive Cancer Care Microarray Facility and the Center for Computational Medicine and Biology centered in the Department of Biomedical Engineering. The services provided by this Core include: mRNA preparation and qualification for kinetic real time PCR (RT-PCR) and microarray experiments; access to canine EST libraries; spotting and hybridization to microarrays, assistance with analysis of microarray data; access to CAGE, a database repository for microarray experiments; primers for RT-PCR; canine tissue qualified antibodies and protocols for their use in Western blotting, immunohistochemical and immunocytochemical applications; cytosolic, sarcolemmal, sarcoplasmic reticulum, ERK1/2 and connexin subproteome preparation and analysis (including the performance of 2D gel electrophoresis (2DE), 2D liquid chromatography (2DLC), differential imaging gel electrophoresis (DICE) and mass spectrometry); and a canine heart-specific protein database for peptide mass fingerprinting (PMF). In addition to the provision of technical expertise to the projects, the core will generate significant cost savings by serving as a centralized resource for molecular biological and protein chemical analysis of the canine heart.

分子遗传学和蛋白质组学核心作为提供专业知识和 协助 Van Eyk 和 Tomaselli 的项目分析基因和蛋白质表达。核心重点是用于微阵列和蛋白质表达研究的性能和分析的 mRNA 和蛋白质的制备和利用。核心设施的活动将在约翰·霍普金斯大学医学院校园内的多个地点进行，包括心脏病学系的实验室、Sidney Kimmel 综合癌症护理微阵列设施以及以该系为中心的计算医学和生物学中心。生物医学工程。该核心提供的服务包括： 动态实时 PCR (RT-PCR) 和微阵列实验的 mRNA 制备和鉴定；访问犬类 EST 库；微阵列点样和杂交，协助分析微阵列数据；访问 CAGE，一个用于微阵列实验的数据库存储库； RT-PCR 引物；犬组织合格的抗体及其在蛋白质印迹、免疫组织化学和免疫细胞化学应用中的使用方案；胞浆、肌膜、肌浆网、ERK1/2和连接蛋白亚蛋白质组制备和分析（包括二维凝胶电泳（2DE）、二维液相色谱（2DLC）、差示成像凝胶电泳（DICE）和质谱的性能）；以及用于肽质量指纹（PMF）的犬心脏特异性蛋白质数据库。除了为项目提供技术专业知识外，该核心还将通过作为犬心脏分子生物学和蛋白质化学分析的集中资源来节省大量成本。