MICROCHEMISTRY and PROTEOMICS

显微化学和蛋白质组学

基本信息

批准号：
7671830
负责人：
PAUL TEMPST
金额：
$ 20.2万
依托单位：
SLOAN-KETTERING INST CAN RESEARCH
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-07-31 至 2012-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7671830
关键词：
Acetylation Affinity Affinity Chromatography Alanine Amino Acid Sequence Amino Acids Anti-Bacterial Agents Antibodies Area Arginine Base Sequence Binding Biological Biological Process Biomedical Research Biotinylation Cell Extracts Cells Chemicals Chemistry Classification Clinical Research Companions Complex Coupling DNA Repair Development Disease Dissection Exhibits Facility Construction Funding Category Generations Glutamine Glycine Goals Growth High Pressure Liquid Chromatography Immunologics Isotopes Label Laboratories Length Ligands MALDI-TOF Mass Spectrometry Manuals Mass Spectrum Analysis Measurement Membrane Memorial Sloan-Kettering Cancer Center Methods Methylation Microchemistry Modeling Modification Molecular Monoclonal Antibodies Mutation N-terminal Nature Normal tissue morphology Nucleic acid sequencing Numbers Pattern Peptide Sequence Determination Peptide Synthesis Peptide Vaccines Peptides Phase Phosphorylation Positioning Attribute Post Translational Modification Analysis Post-Translational Protein Processing Process Protein Analysis Protein Sequence Analysis Proteins Proteomics Purpose Quality Control Radio Radiolabeled Reagent Relative (related person)Reporting Research Research Personnel Research Priority Research Project Grants Retrieval Sampling Scanning Screening procedure Services Signal Pathway Specific qualifier value Specificity Stereoisomer Structure Surface Techniques Vaccines Weight analog base cancer cell cell growth crosslink disulfide bond enantiomer glycosylation immunogenic inhibitor/antagonist instrument instrumentation molecular mass polypeptide programs protein protein interaction radiotracer research study response senescence sulfation synthetic peptide

项目摘要

The Microchemistry and Proteomics Facility (MPF) supports laboratory and clinical research programs at MSKCC by (i) providing synthetic peptides and by (ii) performing mass spectrometric and amino acid sequence analyses of proteins and peptides, generally for the purpose of identification, relative quantitative analysis and post-translational modification (PTM) analysis. Synthetic molecules are made according to specifications provided by the investigator. Samples submitted for identification (either single proteins or mixtures), for PTM analysis or N-terminal sequencing are prepared by the lab requesting service. Information on chemistries and instrumentation, and expert advice on experimental approaches are provided by facility staff when needed. The goal of proteomics is to analyze changing levels, local concentrations and post-translational modifications of proteins in a cell, as well as to analyze higher-order networks of protein-protein interactions. Proteins and protein modifications are critical in the complex signaling pathways and regulatory processes underlying cell growth, division, differentiation, DNA repair, development, senescence, and in responses to bioactive agents, genetic alterations and disease. Dissection of multi-component protein complexes is therefore increasingly the focus of studies on molecular control mechanisms. Identification of unique components provides a powerful approach to the selective retrieval and identification of the companions. The impact that these services have provided, in facilitating research projects and enabling the timely progression of high priority research, can be recognized throughout the reports of the several research programs.

微化学和蛋白质组学设施（MPF）支持实验室和临床研究计划 MSKCC由（i）提供合成肽以及（ii）执行质谱和氨基酸蛋白质和肽的序列分析通常是为了鉴定，相对定量的目的分析和翻译后修饰（PTM）分析。合成分子是根据调查员提供的规格。提交识别的样品（单蛋白或混合物），用于PTM分析或N末端测序，由实验室请求服务制备。有关化学和仪器的信息，以及有关实验方法的专家建议在需要时由设施人员。蛋白质组学的目的是分析变化水平，局部浓度和翻译后细胞中蛋白质的修饰，以及分析蛋白质 - 蛋白质相互作用的高阶网络。蛋白质和蛋白质修饰在复杂的信号通路和调节过程中至关重要潜在的细胞生长，分裂，分化，DNA修复，发育，衰老以及对生物活性剂，遗传改变和疾病。多组分蛋白质复合物的解剖是因此，越来越多地研究分子控制机制的重点。识别独特组件为伴侣的选择性检索和识别提供了强大的方法。这这些服务提供的影响，促进研究项目并及时实现在几项研究的整个报告中，可以认识到高优先研究的进展程序。