Skin Proteomics

皮肤蛋白质组学

• 批准号: 7658257
• 负责人: STEPHEN E BARNES
• 金额: $ 12.93万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7658257
• 关键词: Area; Bioinformatics; Biological; Cells; Complex; Computer software; Condition; Coupled; Databases; Dimensions; Disease model; Electrophoresis; Ensure; Experimental Designs; Fingerprint; Funding; Gel; Generations; Goals; Image Analysis; Isoelectric Focusing; Liquid Chromatography; Mass Spectrum Analysis; Modification; Pilot Projects; Post-Translational Protein Processing; Protein Databases; Protein Sequence Analysis; Proteins; Proteomics; Recovery; Research; Research Personnel; Resolution; Resources; Role; Sampling; Skin; Specimen; Spectrometry, Mass, Electrospray Ionization; Stimulus; Technology; Tissues; Training; University of Alabama at Birmingham Cancer Center; Work; base; instrumentation; member; polypeptide; protein expression; response; size; skin disorder; two-dimensional

DESCRIPTION (provided by applicant): The role of this Core is to provide proteomics and mass spectrometry support for the investigators in the UAB Skin Diseases Research Center. This will be principally in the form of high-resolution separation of proteins in complex biological samples, coupled with mass spectrometry identification of proteins that comprise the biological samples generated by the various projects. Existing instrumentation m the UAB Comprehensive Cancer Center Mass Spectrometry and Proteomics Shared Facility will be used for the Skin Proteomics Core. Because this resource is heavily utilized, funding from the SDRC will enable investigators to obtain the technical help and expertise for their skin related projects. The Core will work with the SDRC investigators of the various projects in experimental design and sample generation, to ensure optimal analysis. Thus, the specific aims of this Core are (1) Optimization of protein recovery from cells and tissue specimens, (2) High resolution protein separation, (3) Isoelectric focusing/SDS-PAGE electrophoresis (on three different 2 nd dimension gel sizes), (4) Qualitative and quantitative gel image analysis via software (currently PDQuest [BioRad]) to identify differential protein expression and/or alterations in posttranslational modifications, (5) MALDI-TOF mass spectrometric identification of polypeptides based on database searching with tryptic mass fingerprints, (6) NanoLC-electrospray ionization mass spectrometry for peptide sequence analysis and analysis of covalent modifications Training in the experimental design and application of proteomics, and (7) use of protein databases and bioinformatics. Overall, the goals of the proteomics core are (1) to provide high-resolution two dimensional electrophoretic and/or chromatographic protein separations coupled with MALDI-TOF and other mass spectrometry analyses, and (2) to identify differences in polypeptide expression and/or modifications that may be the basis for various dermatological conditions being studied by the Center investigators.

描述（由申请人提供）：该核心的作用是提供蛋白质组学和质量 UAB皮肤疾病研究中心的研究人员的光谱学支持。这将主要以复杂生物样品中蛋白质的高分辨率分离的形式，再加上构成各个项目产生的生物样品的蛋白质质谱鉴定。 UAB综合癌症中心质谱法和蛋白质组学共享设施将用于皮肤蛋白质组学核心。由于此资源被大量利用，因此SDRC的资金将使研究人员能够为其与皮肤相关的项目获得技术帮助和专业知识。核心将与实验设计和样本生成各种项目的SDRC研究人员一起工作，以确保最佳分析。因此，该核心的具体目的是（1）优化细胞和组织样品中蛋白质回收的优化，（2）高分辨率蛋白质分离，（3） Isoelectric focusing/SDS-PAGE electrophoresis (on three different 2 nd dimension gel sizes), (4) Qualitative and quantitative gel image analysis via software (currently PDQuest [BioRad]) to identify differential protein expression and/or alterations in posttranslational modifications, (5) MALDI-TOF mass spectrometric identification of polypeptides based on database searching with tryptic mass指纹，（6）纳米 - 电喷雾电离质谱法，用于肽序列分析和分析蛋白质组学实验设计和应用之间的共价修饰训练，以及（7）蛋白质数据库和生物信息学的使用。总体而言，蛋白质组学核心的目标是（1）提供高分辨率的二维电泳和/或色谱蛋白分离，并与MALDI-TOF和其他质谱分析相连，以及（2）以识别多肽表达和/或/或/或/或或/或 修改可能是中心研究人员研究的各种皮肤病学条件的基础。