Phenotyping and Biobanking Core

表型分析和生物样本库核心

基本信息

批准号：
10217068
负责人：
DUNCAN B. CLARK
金额：
$ 57.87万
依托单位：
UNIVERSITY OF PITTSBURGH AT PITTSBURGH
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-07-15 至 2025-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10217068
关键词：
15 year old Acute Adolescence Adolescent Adolescent Development Affect Age Animals Behavioral Biological Biology Brain Breeding Center for Translational Science Activities Characteristics Chronic Circadian Dysregulation Circadian Rhythms Circadian desynchrony Clinical Collection Communities Enrollment Ensure Ethics Fibroblasts Foundations Future Genetic Genetic Heterogeneity Goals Human Impaired cognition Infrastructure Institutes Intervention Investigation Laboratory Animals Magnetic Resonance Measures Modeling Molecular Molecular Profiling National Institute of Drug Abuse Neurosciences Participant Phase Phenotype Physiological Procedures Rattus Reproducibility Research Research Project Grants Research Subjects Research Support Resources Rewards Risk Rodent Rodent Model Sampling Schools Services Sleep Sleep Deprivation Sleep Fragmentations Sleep disturbances Specimen Study models Substance Use Disorder Teenagers Tissue Banks Tissue Sample Universities addiction animal resource base behavioral phenotyping biobank circadian cognitive control cognitive development cohort data management data quality follow-up genome wide association study human tissue innovation novel programs quality assurance recruit repository screening statistics substance use trait translational research program young adult

项目摘要

PROJECT SUMMARY The Center for Adolescent Rhythms, Reward, and Sleep (CARRS) Phenotyping and Biobanking Core (Core B) supports the research projects through the acquisition of research subjects with specific phenotypic characteristics required by the human and animal projects. Our central hypothesis is that late sleep timing, short sleep duration, and circadian misalignment adversely impact cortico-limbic function in adolescents, further enhancing reward sensitivity, impairing cognitive control, and increasing substance use risk. The coordinated and deliberate procedures for assuring analogous phenotypic characteristics in humans and animals provide the foundation for this translational scientific program. For human studies (Projects 1 and 2), Core B will conduct systematic recruitment and screening of adolescents ages 13 through 15 years old to provide participants systematically varied in sleep timing. For rodent studies, Core B will establish a breeding and phenotyping pipeline to provide the animals required for Projects 3, 4 and 5. Core B will also provide biobanking services, including the collection, management, and banking of specimens from adolescent humans and adolescent HS rats to measure molecular rhythm phenotypes and to provide a high-quality repository for future mechanistic studies. Core B contributes to this innovative translational research program by collaboratively developing and implementing plans for assuring analogous phenotypes for CARRS human adolescent and adolescent rodent model studies.

项目概要青少年节律、奖励和睡眠中心 (CARRS) 表型分析和生物样本库核心（核心 B）通过获取具有特定表型的研究对象来支持研究项目人类和动物项目所需的特征。我们的中心假设是晚睡时间，睡眠时间短和昼夜节律失调会对青少年的皮质边缘功能产生不利影响，进一步增强奖励敏感性，损害认知控制，并增加物质使用风险。这协调和深思熟虑的程序，以确保人类和人类具有相似的表型特征动物为这一转化科学计划奠定了基础。对于人类研究（项目 1 和 2），核心B将对13至15岁的青少年进行系统的招募和筛选，以为参与者提供系统性的不同睡眠时间。对于啮齿动物研究，Core B 将建立一个育种和表型分析管道，以提供项目 3、4 和 5 所需的动物。核心 B 还将提供生物样本库服务，包括青少年样本的收集、管理和储存人类和青少年 HS 大鼠测量分子节律表型并提供高质量未来机制研究的存储库。核心 B 为这一创新转化研究项目做出贡献通过合作制定和实施计划来确保 CARRS 人类具有相似的表型青少年和青少年啮齿动物模型研究。