Nanowire Sensor Arrays for Detection of Nucleic Acid Molecules

用于检测核酸分子的纳米线传感器阵列

基本信息

批准号：
7391162
负责人：
Rashid Bashir
金额：
$ 17.33万
依托单位：
PURDUE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-04-01 至 2009-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7391162
关键词：
Address Adsorption All Sites Antibodies Antigens Area Arts Autoimmune Diseases Be++ element Beds Beryllium Binding Biological Biological Models Biological Process Biology Biosensor Boxing Caliber Carbon Cells Charge Chemicals Chemistry Complex Condition DNA DNA Probes DNA Sequence Deoxyribonucleotides Detection Deterioration Device Designs Devices Diagnostic Dimensions Discipline Disease Electrodes Elements Environment Epigenetic Process Equilibrium Figs - dietary Foundations Generations Genetic Goals Health Heating Individual Ions Kinetics Label Lasers Lead Length Liquid substance Malignant Neoplasms Maps Measurement Measures Mediating Medical Medicine MicroRNAs Microfluidic Microchips Microfluidics Modeling Modification Nanotechnology Nucleic Acids Nucleotides Numbers Oligonucleotides Optics Organism Peptide Nucleic Acids Placement Polyethylene Glycols Principal Investigator Proteins RNA Range Reaction Reagent Reporting Research Research Personnel Scheme Semiconductors Sequence Determination Signal Transduction Silicon Single-Stranded DNA Site Source Surface Surface Properties System Systems Integration Techniques Technology Temperature Testing Thinking Time Work Xenobiotics base biochip chemical reaction concept cost density design design and construction detector electron beam lithography high throughput analysis innovation latent nuclear antigen liquid crystal microorganism miniaturize model development nano nanobiotechnology nanofabrication nanoparticle nanoscale nanoscale research nanosensors nanowire new technology novel nucleic acid detection oxidation predictive modeling programs receptor research study scale up sensor simulation small molecule tool

项目摘要

The recent advances in nanotechnology and nanofabrication of semiconductor materials present themselves with new opportunities for miniaturized, ultra-sensitive, label-free, and parallel sensors capable of rapid and highly accurate analysis of biological and chemical entities. Silicon nanowire sensors, based on field effect modulation of current upon binding of molecules on the nanowire surface, have been successfully demonstrated for the detection of DNA and proteins in fluids on individual devices. However, these devices are still to be realized in an array format, in a robust manner using top-down fabrication techniques, with detection of multiple molecules. In this R21 proposal, we have assembled an interdisciplinary team with the goal of designing, producing, functionalizing, and testing the silicon nanowire sensor array. Our work will be motivated by, (a) the need to detect single stranded DNA molecules for sensing and diagnostic applications, and (b) detect microRNA (miRNA) from cell lysates, which have been recently shown to be indicative of diseases such as cancer. These goals will be accomplished by our team of researchers with expertise in nano-scale computation (Ashraf Alam), novel linker design and surface chemistry (Donald Bergstrom), and micro/nano-fabrication (Rashid Bashir). The following are the specific aims of the project: (i) Use novel nanoscale computation approaches to guide the design and fabrication of the nano-wire sensor elements, (ii) Develop novel top-down silicon fabrication techniques to produce nano-wire arrays within microfluidic devices, (iii) Develop novel techniques to functionalize the individual sensors in an array using laser or electrically mediated thermal exhange reactions, and (iv) Performthe detection experiments in an array of nanowires within a microfluidic biochip and explore the use of nano-particle (dendrimer) based charge amplification schemes. Each specific aim includes novel and innovative state-of-the art nanoscale research with broad applications in the area of Nanobiotechnology.

纳米技术的最新进展和半导体材料的纳米化表现出来有新的机会，可用于快速，超敏感，无标签和平行传感器，能够快速和对生物学和化学实体的高度准确分析。硅纳米线传感器，基于现场效应分子在纳米线表面结合后的电流调节已成功证明用于在单个设备上的流体中检测DNA和蛋白质。但是，这些设备使用自上而下的制造技术以强大的方式以良好的方式实现，并以阵列格式实现检测多个分子。在此R21提案中，我们与设计，生产，功能化和测试硅纳米线传感器阵列的目标。我们的工作将是由（a）检测单链DNA分子进行感测和诊断应用的动机，（b）从细胞裂解物中检测MicroRNA（miRNA），最近已证明这表明癌症等疾病。这些目标将由我们具有专业知识的研究人员团队实现纳米级计算（ASHRAF ALAM），新颖的接头设计和表面化学（Donald Bergstrom）和微/纳米制作（Rashid Bashir）。以下是项目的具体目的：（i）使用小说纳米级计算方法指导纳米线传感器元素的设计和制造，（ii）开发新型自上而下的硅制造技术，以在微流体内生产纳米线阵列设备，（iii）开发新技术来使用激光或电介导的热振动反应，（iv）在一个阵列中执行检测实验微流体生物芯片中的纳米线并探索基于纳米颗粒（树枝状聚合物）电荷的使用放大方案。每个特定目标包括新颖和创新的最先进的纳米级研究在纳米生物技术领域具有广泛的应用。