Blood and Tissue Specimen Core Facility

血液和组织样本核心设施

基本信息

批准号：
7658295
负责人：
DANIEL FINK
金额：
$ 5.64万
依托单位：
COLUMBIA UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/7658295
关键词：
Aliquot Applications Grants Area Back Blood Blood specimen Carbon Dioxide Clinical Clinical Chemistry Clinical Laboratory Information Systems Clinical Pathology Clinical Research Clinical Trials Clinical assessments Computer software Condition Consultations Core Facility DNA Databases Devices Diagnostic Doctor of Philosophy Emergency Situation Ensure Epidemiology Equipment Equipment and supply inventories Freezing Hospitals IL6 gene Label Laboratories Liver Manufacturer Name Maps Medical center Medicine Methodology Microbiology Monitor New York Pathologist Pharmacologic Substance Plasma Presbyterian Church Procedures Process Protocols documentation Purpose Quality Control Range Research Design Research Ethics Committees Research Personnel Research Project Grants Retrieval Safety Services Site Specimen Specimen Handling Supervision TNF gene Test Result Testing Tissues Training Translational Research Tube Universities acute stroke computerized data management hospital laboratories instrumentation new technology programs sample collection virology

项目摘要

The aims of the Core D (Blood and Tissue Core) project of the New York Columbia Collaborative Specialized Program on Translational Research In Acute Stroke (NYCC-SPOTRIAS) are to provide centralized, professional management of specimen processing, to perform laboratory testing in a central facility by a laboratory technologist under the supervision of a Clinical Pathologist laboratory director, and to provide a place for long term storage under controlled conditions for DMA and specimen aliquots that can be used for repeat and additional testing. These functions will be performed by the Center for Advanced Laboratory Medicine (CALM) at Columbia University. It is estimated that over the course of the SPOTRIAS project, there will be 680 specimen collection episodes resulting in nearly 7,000 aliquots to be processed, tested and/or stored. Immediately after collection, specimens will be sent to CALM for processing, aliquotting, and DMA extraction. After processing, specimen information will be entered into Freezerworks specimen management software and barcode labels will be generated and placed on the specimens. As the specimens are placed in frozen storage at -80 degrees C, the tubes will be barcode wanded and a specimen map will be created for inventory and retrieval purposes. To ensure the safety of these specimens, CALM will use a monitored freezer on an emergency electrical power circuit with carbon dioxide back-up. Likewise, DNA will be extracted, aliquotted, and prepared for storage in a similar manner. CALM will perform hs-CRP, IL6, and TNF testing on retrieved aliquots. All testing will be performed in duplicate and with appropriate Quality Control procedures using commercial controls and duplicate specimens; the laboratory will also subscribe to available proficiency programs. CALM staff will oversee the delivery of specimens to the hospital laboratory for CK, Liver Profile and other tests as required; CALM staff will also retrieve the results of these tests and enter them into a computerized database for transfer to the data management staff.

纽约哥伦比亚哥伦比亚急性中风转化研究（NYCC-SPOTRIAS）的核心D（血液和组织核心）项目的目的是提供标本处理的集中式专业管理，以在中央进行实验室测试在临床病理学家实验室主管的监督下，实验室技术专家的设施由DMA和标本等分试样的长期存储提供了一个可以用于重复和其他测试的地方。这些功能将由哥伦比亚大学高级实验室医学中心（CALM）进行。据估计，在整个现场项目的过程中，将有680个标本收集剧集，导致将近7,000个等分试样，测试和/或存储。收集后，立即将标本送到平静下来进行处理，等分试样和DMA提取。处理后，标本信息将输入Freezerworks标本管理软件，并将生成条形码标签并将其放置在样品上。当样品放置在-80度C的冷冻存储中时，将划分管道，并为库存和检索目的而创建标本图。为了确保这些标本的安全性，平静将在带有二氧化碳备份的紧急电力电路上使用受监控的冰柜。同样，DNA也会被提取，等分并准备以类似的方式存储。冷静将对检索的等分试样进行HS-CRP，IL6和TNF测试。所有测试将使用商业控制和重复标本进行重复和适当的质量控制程序进行；该实验室还将订阅可用的能力计划。平静的员工将根据需要监督标本向医院实验室提供的CK，肝脏轮廓和其他测试；平静的员工还将检索这些测试的结果，并将其输入计算机数据库，以转移到数据管理人员。