Physiological Assessment

生理评估

• 批准号: 7648214
• 负责人: Elisabeth R Barton
• 金额: $ 18.6万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7648214
• 关键词: Animals; Biological Assay; Breeding; Cell Therapy; Communities; DYSF gene; Development; Equipment; Evaluation; Genes; Grant; Hindlimb Suspension; Housing; In Situ; In Vitro; Individual; Injury; Laboratories; Life; Longitudinal Studies; Measurement; Mechanics; Monitor; Motivation; Mus; Muscle; Muscle function; Muscular Dystrophies; Myotonic Dystrophy; Numbers; Outcome; Physiological; Protocols documentation; Research; Resources; Running; Sarcoglycans; Testing; Therapeutic; experience; instrumentation; mouse model; muscular dystrophy mouse model; pre-clinical; research study

Critical to the evaluation of potential therapeutics (pharmacological, gene or cell therapies) in mouse models of muscular dystrophy are sensitive and repeatable physiological assessments of muscle function. Therefore, we are proposing to create a research core which performs ex vivo, in situ, and whole animal assessments of muscle integrity and function. Three of the projects (Projects 1-3) proposed in this grant contain components that require muscle physiological measurements. (Project 4 relies on the outcome of these tests for pre-clinical justification.) The Physiological Assessment Core will meet the needs of the projects within this grant, as well as the needs of the muscular dystrophy research community. In addition, the core will maintain in house colonies of mouse models of DMD, LGMD, and myotonic dystrophy in order to support studies where long-term testing is necessary. The resources of this core are contained within Dr. Sweeney's laboratory, which includes all instrumentation for muscle physiological measurements, as well as specialized equipment for hindlimb suspension experiments, documented free wheel running, and treadmill running. Dr. Barton is a muscle physiologist with extensive experience evaluating muscle function in mice. Monica Zadel is an expert at performing muscle mechanical experiments, while Pedro Acosta maintains our breeding colonies and will monitor hindlimb suspension and free wheel running experiments. We believe that this Core will provide a valuable resource that will enable the entire muscular dystrophy research community to utilize these assays to assess the potential benefits of a large number of approaches to the treatment of different forms of muscular dystrophy

对于评估小鼠模型中潜在疗法（药理，基因或细胞疗法）的评估至关重要 肌肉营养不良的肌肉功能是敏感且可重复的生理评估。 因此，我们提议创建一个研究核心，该研究核心在原位和整个动物 评估肌肉完整性和功能。本赠款中提出的三个项目（项目1-3） 包含需要肌肉生理测量的成分。 （项目4依赖于 这些用于临床前依据的测试。）生理评估核心将满足 这笔赠款中的项目以及肌肉营养不良研究社区的需求。此外， 核心将在DMD，LGMD和Myotonic营养不良的鼠标模型的房屋菌落中保持 支持需要长期测试的研究。该核心的资源包含在Dr. Sweeney的实验室，其中包括所有用于肌肉生理测量的仪器，以及 后肢悬架实验的专业设备，有记录的自由车轮运行和跑步机 跑步。 Barton博士是一位肌肉生理学家，拥有丰富的经验，评估小鼠的肌肉功能。 莫妮卡·扎德尔（Monica Zadel）是执行肌肉机械实验的专家，而佩德罗（Pedro Acosta）保持我们的 繁殖菌落，并将监测后肢悬架和自由车轮跑步实验。 我们认为，该核心将提供宝贵的资源，以使整个肌肉营养不良 研究社区利用这些测定法来评估大量方法的潜在好处 治疗不同形式的肌营养不良症