Organ Specific Project - Kidney

器官特定项目 - 肾脏

基本信息

批准号：
10201589
负责人：
RAYMOND C. HARRIS
金额：
$ 115.85万
依托单位：
VANDERBILT UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-09-24 至 2022-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10201589
关键词：
3-Dimensional APOL1 gene Address Affect African American American Anatomy Antibodies Atlases Biological Assay Biology Blood capillaries Cell Separation Cells Characteristics Chronic Kidney Failure Clinical Collection Cytometry Data Development Disease susceptibility Dissociation Elements Emerging Technologies Ensure Epidemiology Excision Extracellular Matrix Filtration Fluorescence Gene Expression Profiling Genetic Goals Health Heterogeneity Histologic Homeostasis Human Human BioMolecular Atlas Program Image Imaging technology Immunohistochemistry Incidence Individual Investigation Kidney Kidney Diseases Knowledge Link Lipids Lymphatic Magnetic Resonance Imaging Mass Spectrum Analysis Methods Mission Molecular Molecular Analysis Molecular Profiling Morphology Multimodal Imaging Nephrons Operative Surgical Procedures Organ Personal Satisfaction Preparation Property Proteins Resolution Risk Sampling Sodium Chloride Source Specimen Standardization Structure Tissue Banks Tissues Tubular formation Universities Variant Water assay development base biobank blood pressure regulation cell type data management experimental study extracellular genetic association genetic variant human tissue imaging modality insight interstitial kidney cell male microCT molecular imaging multimodality programs racial disparity scale up sex surgery material three dimensional structure

项目摘要

Project Summary The goal of the Kidney OSP is to address the limitation in current knowledge of kidney biology by developing an ultra-high content mass spectrometry (MS)-based 3-dimensional (3-D) imaging pipeline to characterize the molecular signatures (lipids, metabolites and proteins) of different cell types and extracellular matrix components at high resolution in the intact human kidney. The imaging data acquired by this unique platform will be aligned and fused with the 3-D structure of the human kidney obtained by coordinate-based alignment with MRI, CT and histochemical based images. This technological pipeline will be developed to be compatible with other molecular interrogation pipelines generated through HuBMAP to create a comprehensive multidimensional 3-D molecular atlas of the normal human kidney.

项目概要肾脏 OSP 的目标是通过开发解决当前肾脏生物学知识的局限性基于超高内涵质谱 (MS) 的 3 维 (3-D) 成像管道，用于表征不同细胞类型和细胞外基质的分子特征（脂质、代谢物和蛋白质）完整人体肾脏的高分辨率成分。通过这个独特的平台获取的成像数据将与通过基于坐标的对齐获得的人类肾脏的 3D 结构对齐并融合具有 MRI、CT 和基于组织化学的图像。该技术管道将被开发为兼容与通过 HuBMAP 生成的其他分子询问管道一起创建全面的正常人肾脏的多维 3D 分子图谱。