Biorepository Core B

生物样本库核心B

基本信息

批准号：
10361544
负责人：
CLAUDIO FIOCCHI
金额：
$ 22.01万
依托单位：
CASE WESTERN RESERVE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-02-15 至 2025-01-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10361544
关键词：
Adipocytes Area Basic Science Biology Biopsy Cancer Control Cell Separation Cells Chronic Classification Clinic Clinical Clinical Data Clinical Investigator Clinical Laboratory Information Systems Clinical Research Colon Carcinoma Constipation Consultations Consumption Crohn&apos s disease DNA Data Analyses Diagnosis Digestive System Disorders Diverticulosis Elements Epidemiology Epithelial Cells Esophagus Experimental Designs Familial Adenomatous Polyposis Syndrome Feces Flow Cytometry Foundations Funding Gastrointestinal tract structure Genealogy Generations Genetic Genotype Goals Human Human Resources In Vitro Individual Inflammation Institution Institutional Review Boards Intestinal Volvulus Intestines Large Intestine Liver diseases Logistics Malignant Neoplasms Malignant neoplasm of esophagus Mesentery Metabolism Methods Monitor Operative Surgical Procedures Organoids Patients Performance Phenotype Physicians Process Research Research Activity Research Institute Research Personnel Research Priority Research Project Grants Resources Retrieval Running Sampling Scientist Serum Services Small Intestines Specimen Supervision System Time Tissues Training Translating Translational Research Ulcerative Colitis Universities bench to bedside biobank cell type community center cost effective cost effectiveness design experience gastrointestinal genomic platform improved member monolayer patient oriented research research study single cell analysis success synergism translational study tumorigenesis

项目摘要

BIOREPOSITORY CORE B PROJECT SUMMARY Dr. Claudio Fiocchi is Core Director. Dr. Florian Rieder serves as Associate Director, and Drs. Jean-Paul Achkar, Jennifer Ko and Amitabh Chak serve as Technical Directors. Goals of the Biorepository Core: The specific goals include: 1) Procure, process, and distribute tissue and cells from surgical specimens and biopsies, as well as serum, DNA and stools, from patients with gastrointestinal inflammation, cancer and controls for use in IRB-approved projects of DDRCC members; 2) Provide phenotypic, genotypic, clinical and demographic information of the biosamples; 3) Provide isolation, purification, culture and use of most types of gastrointestinal tract cells; 4) Advise, teach, and supervise investigators on principles, methods, analysis and experimental design; 5) Monitor Core services and continuously evaluate the needs of the DDRCC members to maximally benefit their research priorities, and 6) provide all above services in the most cost-effective and least time-consuming manner. Benefits of the Biorepository Core: During the first 4 years of funding, the objective evidence for the essential benefits of the Cleveland DDRCC Biorepository Core is the fact not a single investigator of the large DDRCC community has failed to receive the type, size or number of biospecimens, as well as the training and logistic support required for his/her studies. This success was achieved by the careful enactment of the specific goals. A new benefit of the Core is a state-of-the-art laboratory information system (LabVantage Solutions-LV), which tracks origin of samples, clinical information, genealogy of samples, and sample retrieval and distribution, and has connectivity to EPIC, Copath, Sunquest, etc. This new system greatly benefits Core users by assuring specimen quality, quick identification, diagnosis and delivery, and formal access to clinical data for interpretation of the experimental results. Finally, the Core has considerably expanded its technical services to include 1) generation of intestinal organoids, 2) organoid-derived epithelial cell monolayers, 3) isolation of mesenteric adipocytes, 4) single cell analysis (Fluidigm C1 and 10X Genomics platforms), 5) CyTOF and spectral flow cytometry systems, and 6) access to a large biobank of esophageal and colon cancer specimens. Overall, the Core benefits DDRCC members by providing unique and high quality biosamples and services.

生物储存库核心 B 项目摘要 Claudio Fiocchi 博士是核心主任。 Florian Rieder 博士担任副主任，Drs.让-保罗 Achkar、Jennifer Ko 和 Amitabh Chak 担任技术总监。生物样本库核心的目标：具体目标包括： 1) 采购、处理和分发组织和来自胃肠道疾病患者的手术标本和活组织检查以及血清、DNA 和粪便中的细胞用于 DDRCC 成员的 IRB 批准项目的炎症、癌症和控制； 2）提供表型，生物样本的基因型、临床和人口统计信息； 3）提供分离、纯化、培养和使用大多数类型的胃肠道细胞； 4）就原则向调查人员提供建议、教导和监督，方法、分析和实验设计； 5) 监控核心服务并持续评估需求 DDRCC 成员最大限度地受益于他们的研究重点，并且 6) 在最具成本效益和最省时的方式。生物样本库核心的好处：在资助的前 4 年里，客观证据表明克利夫兰 DDRCC 生物样本库核心的基本优势是，没有一个大型研究人员 DDRCC 社区未能收到生物样本的类型、大小或数量，以及培训和培训他/她的学习所需的后勤支持。这一成功是通过仔细制定具体措施而取得的目标。 Core 的一个新优势是最先进的实验室信息系统 (LabVantage Solutions-LV)，跟踪样本来源、临床信息、样本谱系以及样本检索和分布，并可连接到 EPIC、Copath、Sunquest 等。这个新系统极大地惠及了核心用户通过确保样本质量、快速识别、诊断和交付以及正式访问临床数据实验结果的解释。最后，核心将其技术服务大大扩展至包括 1) 肠道类器官的生成，2) 类器官衍生的上皮细胞单层，3) 分离肠系膜脂肪细胞，4) 单细胞分析（Fluidigm C1 和 10X Genomics 平台），5) CyTOF 和光谱流式细胞术系统，以及 6) 获取食管癌和结肠癌样本的大型生物库。总体而言，核心通过提供独特且高质量的生物样本和服务使 DDRCC 成员受益。