Core C

核心C

• 批准号: 10199029
• 负责人: Elda Arrigoni
• 金额: $ 17.38万
• 依托单位: BETH ISRAEL DEACONESS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10199029
• 关键词: Bathing; Brain; Calcium; Cells; Characteristics; Clozapine; Code; Core Facility; Cre driver; Data; Electrophysiology (science); Ensure; Graph; Histological Techniques; Histology; Image; Imaging Techniques; In Vitro; Ivermectin; Laboratories; Light; Measures; Monitor; Mus; Nerve; Neurons; Neurotransmitters; Oxides; Physiologic pulse; Population; Population Study; Property; Protocols documentation; Publications; Publishing; Reporter; Research Design; Research Personnel; Resource Sharing; Resources; Services; Sleep Apnea Syndromes; Slice; Statistical Data Interpretation; Synapses; Techniques; Update; Whole-Cell Recordings; Work; adeno-associated viral vector; calcium indicator; designer receptors exclusively activated by designer drugs; efficacy testing; experimental study; extracellular; imaging study; in vivo; neurochemistry; novel; optogenetics; patch clamp; postsynaptic; postsynaptic neurons; receptor; response; tool

Project Summary/Abstract- Core C The in vitro Electrophysiology Core (Core C) will provide services for in vitro electrophysiological studies using novel tools such as optogenetics, designer receptors exclusively activated by designer drugs (DREADDs) and in vitro GCaMP6 calcium imaging. We will prepare brain slices, conduct patch-clamp recordings, optimize the protocols for the optogenetic and chemogenetic experiments, perform Ca2+ imaging studies in in vitro slices, and conduct channelrhodopsin2 (ChR2)-assisted circuit mapping (CRACM). The Core will also perform histology of the recorded slices, and analyze the associated data. Project 1 (Saper), Project 2 (Scammell), Project 3 (Fuller) and Project 4 (Vanderhost and Dymecki) will use this Core facility. These projects are not equipped to conduct in vitro electrophysiology recordings and there are no institutional resources that can provide this service. The Projects will benefit from the expertise and shared resources of the Core.

项目总结/摘要 - 核心 C 体外电生理核心（Core C）将为体外 使用光遗传学、设计受体等新型工具进行电生理学研究 仅由设计药物 (DREADD) 和体外 GCaMP6 钙激活 成像。我们将准备脑切片，进行膜片钳记录，优化 光遗传学和化学遗传学实验方案，执行 Ca2+ 成像 体外切片研究，并进行视紫红质通道 2 (ChR2) 辅助回路 映射（CRACM）。核心还将对记录的切片进行组织学分析，并且 分析相关数据。项目 1 (Saper)、项目 2 (Scammell)、项目 3 (Fuller) 项目 4（Vanderhost 和 Dymecki）将使用此核心设施。这些项目是 不具备进行体外电生理学记录的能力，并且没有 可以提供这种服务的机构资源。该项目将受益于 核心的专业知识和共享资源。