THIAMINE INFLUENCE ON WALLEYE REPRODUCTION AND CHINOOK SALMON EMBRYOS

硫胺素对白眼鱼繁殖和奇努克鲑鱼胚胎的影响

• 批准号: 7610299
• 负责人: MICHAEL H ZEHFUS
• 金额: $ 1.85万
• 依托单位: UNIVERSITY OF SOUTH DAKOTA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7610299
• 关键词: Biological Assay; Chinook Salmon; Computer Retrieval of Information on Scientific Projects Database; Embryo; Enzymes; Fishes; Food; Funding; Grant; Institution; Licensing; Measures; Modeling; Radioactive; Radioactivity; Reproduction; Research; Research Personnel; Resources; Salmon; Source; System; Testing; Thiamine; Thinking; United States National Institutes of Health; Universities; Work; detector; theories; thiaminase II

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. It has been proposed that Lake Oahe Walleye and Salmon have low levels of thiamine because they ingest a thiaminase in their food. Thiaminase is an enzyme that destroys thiamine and is thought to exist in the gut of some of the smaller fish that the Salmon and Walleye prey upon. We cannot test this theory, however, because the presently accepted assay for thiaminase activity involves the use of radioactive materials, and Black Hills State University does not have a license to handle such material. This project seeks develop a non-radioactive thiaminase assay that will work using the GC/MS system that is already in use at Black Hills State. This assay will be closely modeled after the radioactivity assay, but in the last step, instead of determining the amount of thiazoethanol liberated by the thiaminase using radioactivity, we propose to measure the thiazoethonol using using mass sensitive detector built into our GC/MS system.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 有人提出，Oahe Lake Walleye湖和鲑鱼的硫胺素水平较低，因为他们在食物中摄入了硫胺素酶。 硫胺素酶是一种破坏硫胺素的酶，被认为存在于鲑鱼和角膜白斑猎物的一些较小鱼的肠道中。 但是，我们无法检验该理论，因为目前接受的硫胺素酶活性的测定法涉及放射性材料的使用，而黑山州立大学则没有执行此类材料的许可。 该项目寻求开发一种非放射性硫胺素酶测定法，该测定法将使用Black Hills State已使用的GC/MS系统来起作用。 该测定法将在放射性测定后进行密切建模，但在最后一步中，我们建议使用放射性确定硫胺素酶释放的噻唑乙醇的量，而是我们建议使用使用内置在我们的GC/MS系统中的质量敏感探测器来测量噻唑乙醇。