COBRE: UOK HSC: P2: ANALYSIS OF BIOFILMS ON NATURAL & SYNTHETIC DENTAL SURFACES

COBRE：UOK HSC：P2：天然生物膜分析

• 批准号: 7610545
• 负责人: Sharukh S Khajotia
• 金额: $ 22.38万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7610545
• 关键词: 3-Dimensional; Adhesions; Anti-Bacterial Agents; Atomic Force Microscopy; Biocompatible Materials; Characteristics; Chemicals; Computer Retrieval of Information on Scientific Projects Database; Cost Savings; Dental; Dental Cementum; Dental Enamel; Dental caries; Development; Disease; Drug Formulations; Funding; Goals; Grant; Guidelines; Hydrophobicity; In Vitro; Incidence; Institution; Investigation; Knowledge; Lasers; Measures; Mechanics; Microbial Biofilms; Microscope; Modification; Oral cavity; Patients; Periodontal Diseases; Polishes; Polymers; Research; Research Personnel; Resources; Role; Scanning; Source; Streptococcus gordonii; Streptococcus mutans; Surface; Testing; Tooth structure; United States National Institutes of Health; Wettability; base; improved; microbial; microbial colonization; monomer; oral care; physical property; restoration; restorative material; success; tooth surface

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Microbial colonization of exposed tooth surfaces (enamel and cementum) in the oral cavity has been demonstrated to be associated with biofilm formation and subsequent periodontal disease or dental caries. Studies have demonstrated that tooth surfaces and the surfaces of certain dental biomaterials that have higher surface roughness values have increased microbial attachment and adhesion in comparison with similar substrates that have been polished or glazed. However, initial investigations into the reduction of surface roughness or incorporation of antibacterial monomers in polymer-based restorative biomaterials have had limited success in reducing biofilm formation. The overall goal of this project is to determine the utility of selected topographical and physical properties as predictors of in vitro biofilm formation by Streptococcus mutans and by Streptococcus gordonii. The specific aims of this investigation are: 1) to characterize selected topographical and physical properties of tooth and restorative biomaterial surfaces and to measure biofilm accumulations of S. mutans and S. gordonfi on those surfaces, and 2) to quantify the effect of laser, chemical and mechanical treatments of tooth and restorative biomaterial surfaces on selected topographical and physical properties and S. mutans and S. gordonfi biofilm accumulation. The studies comprising this project will utilize atomic force microscopy to obtain non-destructive 3-dimensional topographical and nanohardness characteristics of the surfaces tested. A contact angle goniometer will be used to measure the surface energy and wettability/hydrophobicity of the substrates, and a confocal laser scanning microscope will be used to quantify the biofilms formed in an in vitro batch culture apparatus. It is hypothesized that accurate characterization of the topography of natural and synthetic dental substrates will provide significant fundamental information on the role of those surfaces in biofilm development, particularly in the proximity of restorations. The knowledge gained from this project will also be useful in the modification of the topography of existing dental restorative materials and periodontal treatments, or in the formulation of guidelines for new restorative materials and treatments. In the long-term, this knowledge could help to reduce the incidence of disease connected with biofilm formation, thereby realizing substantial cost savings and improved oral care for dental patients.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 已经证明，口腔中裸露的牙齿表面（牙釉质和胶质）的微生物定植已证明与生物膜形成以及随后的牙周疾病或牙龋有关。研究表明，与已抛光或釉面相似的类似底物相比，具有较高表面粗糙度的某些牙齿生物材料的表面和某些牙齿生物材料的表面具有增加的微生物附着和粘附。但是，对基于聚合物的恢复性生物材料中抗菌单体的表面粗糙度或掺入的最初研究在减少生物膜形成方面的成功有限。该项目的总体目标是确定所选的地形和物理特性的实用性，作为链球菌突变体和gordonii链球菌形成体外生物膜形成的预测指标。这项研究的具体目的是：1）表征牙齿和恢复性生物材料表面所选的地形和物理特性，并测量链球菌链球菌和戈尔多菲链球菌对这些表面上的生物膜积累，以及2）量化激光，化学和机械性能的牙齿和机械性能的效果，并量化了牙齿和机械性能的构图和型号的杂物，并s。积累。包括该项目的研究将利用原子力显微镜获得所测试表面的非破坏性三维地形和纳米性特征。接触角将使用底物的表面能量和润湿性/疏水性测量，并将使用共聚焦激光扫描显微镜来量化体外批处理培养物中形成的生物膜。假设对自然和合成牙科底物的地形的准确表征将提供有关这些表面在生物膜发育中的作用的重要基本信息，尤其是在恢复的附近。从该项目中获得的知识也将有助于修改现有牙科修复材料和牙周处理的地形，或制定新的修复材料和处理的指南。从长远来看，这些知识可以帮助减少与生物膜形成有关的疾病的发生率，从而实现大量的成本节省并改善牙科患者的口腔护理。