COBRE: UNV MED SCH: CORE B: MOLECULAR EXPRESSION & TRANGENICS

COBRE：UNV MED SCH：核心 B：分子表达

• 批准号: 7610555
• 负责人: William T Gerthoffer
• 金额: $ 24.69万
• 依托单位: UNIVERSITY OF NEVADA RENO
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7610555
• 关键词: Adenovirus Vector; Bathing; Cells; Computer Retrieval of Information on Scientific Projects Database; Consult; Daily; Electroporation; Equipment; Funding; Gene Delivery; Gene Transfer; Grant; In Situ; Incubators; Institution; Mechanics; Messenger RNA; Methods; Molecular; Muscle; Plasmids; Postdoctoral Fellow; Problem Solving; Production; Progress Reports; Proteins; Reagent; Research; Research Personnel; Resources; Reverse Transcriptase Polymerase Chain Reaction; Smooth Muscle Myocytes; Source; Time; Tissues; Training; Transgenic Mice; Transgenic Organisms; United States National Institutes of Health; Viral Packaging; Viral Vector; Virus; Water; Western Blotting; Work; design; gene cloning; gene transfer vector; interest; professor; protein expression; transgene expression; vector

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Molecular Expression and Transgenic Core (Core B) will have three major functions: 1) To provide adenoviral gene transfer vectors and protein transduction reagents; 2) To assess mRNA and protein expression for all projects by RT-PCR and western blotting and 3) To procure transgenic mice and maintain transgenic mouse lines. Dr. William T. Gerthoffer will direct the core. He will work closely with Dr. Cherie Singer, Research Assistant Professor and two senior Research Associates to organize daily activities of the Core lab. The Director will consult with Project PIs during monthly progress reports and will solve problems that arise with production or quality of the gene transfer and protein transduction vectors. Dr. Cherie Singer, Research Assistant Professor will design all requested plasmid and virus constructs and will supervise Shanti Rawat, Research Associate who will serve as the chief technician for vector production. Mrs. Rawat will clone genes of interest, produce bacterial ceils for protein expression and propagation of plasmids and build adenovirus vectors. She will be assisted by a second full-time technician, Mariam Ba, who will assist in propagating bacterial and viral vectors and viral packaging cells. Ms Ba will also assist project PIs in assessing transgene expression in cultured smooth muscle cells and tissues by RT-PCR and western blotting. She is also trained in basic muscle mechanics and tissue electroporation methods for gene delivery in situ. Core equipment (hoods, incubator, water bath, centrifuges)will also be maintained by the Core B staff.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 分子表达和转基因核（核B）将具有三个主要功能：1） 提供腺病毒基因转移载体和蛋白质转导试剂； 2）评估mRNA和蛋白质 RT-PCR和Western blotting对所有项目的表达以及3）采购转基因小鼠并保持 转基因小鼠线。 William T. Gerthoffer博士将指导核心。他将与Cherie博士紧密合作 歌手，研究助理教授和两名高级研究协会组织的日常活动 核心实验室。董事将在每月进度报告中咨询PIS项目，并将解决问题 这是由基因转移和蛋白质转导载体的产生或质量产生的。 Cherie Singer博士， 研究助理教授将设计所有要求的质粒和病毒构造，并将监督Shanti 拉瓦特（Rawat），研究助理，将担任媒介生产的首席技术人员。拉瓦特夫人威尔 感兴趣的克隆基因，生产细菌天花板，用于蛋白质表达和质粒的传播并构建 腺病毒载体。她将得到第二任全职技术员Mariam BA的协助，他将协助 传播细菌和病毒载体以及病毒包装细胞。 BA女士还将协助PIS项目 通过RT-PCR和Western印迹评估培养的平滑肌细胞和组织中的转基因表达。她还接受了基本肌肉力学和组织电穿孔方法的训练，用于原位基因递送。 核心设备（引擎盖，孵化器，水浴，离心机）也将由核心B员工维护。