Structure and Function of Ascaris Neuropeptides

蛔虫神经肽的结构和功能

基本信息

批准号：
7559604
负责人：
ANTONY O. STRETTON
金额：
$ 34.05万
依托单位：
UNIVERSITY OF WISCONSIN-MADISON
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-02-15 至 2011-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7559604
关键词：
Affect Affinity Antibodies Area Ascaris Ascaris suum Basic Science Behavior Biochemical Caenorhabditis elegans Cells Dorsal Drug resistance Family Ganglia Generations Genetic Grant High Pressure Liquid Chromatography In Situ Hybridization Indium Individual Interneurons Lasers Locomotion MALDI-TOF Mass Spectrometry Mass Spectrum Analysis Mediating Methods Molecular Molecular Biology Morphology Motor Motor Activity Motor Neurons Movement Muscle Muscle Cells Muscle Tension Myxoid cyst Nematoda Nerve Nerve Tissue Nervous system structure Neurons Neuropeptide Gene Neuropeptides Paralysed Parasitic nematode Peptides Pharmaceutical Preparations Physiological Play Positioning Attribute Process Relaxation Reporting Research Role Sampling Signal Transduction Site Source Structure System Techniques Testing Time Tissues Transcript Work base cellular targeting designer antibody falls immunocytochemistry in vivo interdisciplinary approach molecular mass neuromuscular system neuronal cell body novel protein aminoacid sequence relating to nervous system research study

项目摘要

The locomotory system of nematodes is the target of many currently used anthelminthics, since disruption of locomotory behavior interferes with the normal processes by which many nematodes maintain their position within the host. The problem of genetic drug resistance is making many of the available drugs less effective, so it is important to develop new generations of anti-nematdde drugs. The proposed research aims to describe new intercellular signaling systems that are important in the motornervous system that controls locomotion. Our strategy is based on our previous work in which we isolated and sequenced a large family of 29 novel endogenous neuropeptides in the parasitic nematode, Ascaris suum. Preliminary experiments have shown that almost all of the peptides tested have potent activity .on the neuromuscular system of Ascaris. Some cause paralysis, others exaggerate locomotory movements. Neuropeptides, therefore, play an important role in the overall activity of the motornervous system. There is every indication that there are many more peptides than the ones we have isolated so far. There is no indication that the currently available peptides are the most potent, so we propose to isolate new peptides, concentrating on those that are present in the motornervous system, in order to detect new peptides that have potent physiological activity. This is basic research upon which new generations of anthelminthics can ultimately be developed.

线虫的机车系统是许多当前使用的驱动器的目标，因为运动行为的破坏会干扰许多线虫维持的正常过程他们在主机中的位置。遗传耐药性的问题是使许多可用的药物效率较低，因此重要的是开发新一代的抗Nematdde药物。拟议的研究旨在描述在整体系统中很重要的新的细胞间信号传导系统控制运动。我们的策略是基于我们以前的工作，在该工作中，我们隔离并测序了一个大型 29个新型内源性神经肽在寄生线虫suum中。初步的实验表明，几乎所有已测试的肽都具有有效的活性。 Ascaris系统。有些引起瘫痪，另一些则夸大了运动。神经肽，，因此，在整体系统的整体活动中发挥重要作用。有每一个迹象比我们到目前为止孤立的肽要多得多。没有迹象表明目前可用的肽是最有效的，因此我们建议隔离新肽，专注于为了检测具有有效性的新肽生理活动。这是基础研究，新一代的驱星机构最终可以成为发达。