CELLULAR AND MOLECULAR STUDIES ON ASCARIS NEUROPEPTIDES

蛔虫神经肽的细胞和分子研究

• 批准号: 2390272
• 负责人: ANTONY O. STRETTON
• 金额: $ 19.03万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-07-01 至 1999-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2390272
• 关键词: Ascaris; antibody specificity; biological signal transduction; cell cell interaction; electrophysiology; high performance liquid chromatography; laboratory mouse; laboratory rabbit; mass spectrometry; monoclonal antibody; motor neurons; neural information processing; neuroanatomy; neurobiology; neuromuscular function; neuropeptides; neurophysiology; protein structure function; tissue /cell culture

The overall aim of the proposed project is to determine the role of endogenous neuropeptides in the control of behavior in the nematode Ascaris. There is a wide diversity of neuropeptides in Ascaris, comprising at least 16 families. The family of FMRFamide-like peptides includes at least 15 members. In the proposed grant period we plan to determine the role of Ascaris FMRFamide-like peptides by determining their site of action at the single cell level. Using specific antibodies we will also identify the individual neurons that contain each of these peptides, thus identifying both halves of the intercellular signaling system mediated by each neuropeptide. We also plan to use several novel strategies to discover new families of neuropeptides; these will include bioassay,an immunoassay that will recognize peptides unrelated to previously identified peptides, and the application of several chemical modifications. Gaining a basic understanding of the Ascaris nervous system is important since Ascaris is a model nematode in which to investigate the control of locomotion in nematodes. The Ascaris motor nervous system is anatomically similar to that of other nematodes, so it is reasonable to assume that this similarity will also apply at the functional level. By studying the mechanisms underlying the activity of the neuromuscular system of Ascaris, and the role of neuropeptide in controlling this activity, general principles applying to other nematodes will be found. This basic understanding of the nematode nervous system will make a contribution to parasite biology, and allow differences in the biology between host and parasite to be identified on food supplies, and the problems they cause as animal and human parasites, especially in tropic and economically less- developed countries, this is an important health-related goal.

拟议项目的总体目的是确定 在线虫中控制行为的内源性神经肽 阿斯卡里斯。 assaris中有广泛的神经肽，包括 至少有16个家庭。 FMRFAMIDE样肽的家族包括 至少15名成员。在拟议的赠款期内，我们计划确定 Ascaris fmrfamide样肽的作用通过确定其位置 在单细胞水平上的作用。 使用特定抗体，我们也将 识别包含每种肽的单个神经元，因此 识别由 每个神经肽。我们还计划使用几种新颖策略来 发现新的神经肽家族；这些将包括生物测定 免疫测定将识别与以前无关的肽 确定的肽，并应用了几种化学物质 修改。 对Ascaris神经系统的基本了解很重要 由于assaris是一个模型线虫，在其中研究对控制的控制 线虫中的运动。 Ascaris运动神经系统在解剖学上是 与其他线虫类似，因此可以合理地假设 这种相似性也将适用于功能级别。 通过研究 Ascaris神经肌肉系统活性的机制， 神经肽在控制这一活性中的作用，一般 将发现适用于其他线虫的原则。 这个基本 对线虫神经系统的理解将为 寄生虫生物学，并允许宿主和 寄生虫在食品供应上被发现，以及它们引起的问题 动物和人类寄生虫，特别是在热带和经济上 - 发达国家，这是与健康相关的重要目标。