CORE--NEUROCYTOLOGY /CELLULAR IMAGING

核心--神经细胞学/细胞成像

• 批准号: 7668701
• 负责人: PAUL E MICEVYCH
• 金额: $ 18.1万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7668701
• 关键词: Address; Adult; Affect; Animal Model; Arts; Axon; Behavior; Biological Sciences; Biomedical Research; Breeding; Caring; Cell Transplantation; Cells; Cellular biology; Communities; Confocal Microscopy; Consultations; Cost Savings; Cultured Cells; Cytology; Data; Degenerative Disorder; Development; Disease; Educational process of instructing; Embryo; Equipment; Equipment and Supplies; Faculty; Fishes; Fluorescence; Genetic; Genetic Screening; Genome; Histology; Housing; Human Resources; Image; Imagery; Immunofluorescence Microscopy; In Situ Hybridization; In Vitro; Individual; Injection of therapeutic agent; Investigation; Label; Laboratories; Lasers; Lead; Learning; Left; Life; Malignant Neoplasms; Metabolic; Methodology; Methods; Microdissection; Microscope; Minnesota; Modeling; Molecular; Morphology; Multipotent Stem Cells; Natural regeneration; Nerve Degeneration; Neuraxis; Neurodegenerative Disorders; Neuroglia; Neurons; Neurosciences; Neurosciences Research; Outcome; Pathogenesis; Performance; Population; Postdoctoral Fellow; Preparation; Process; Purpose; Range; Reagent; Recruitment Activity; Research Design; Research Personnel; Scanning; Services; Slice; Stem cells; Supervision; System; Techniques; Therapeutic; Time; Tissues; Training; Transgenes; Translational Research; Transplantation; Universities; Vertebrates; Work; Zebrafish; cellular imaging; cost; design; developmental disease; embryonic stem cell; experience; immunocytochemistry; in vivo; instrument; interdisciplinary approach; interest; member; mutant; nerve stem cell; nervous system development; neurocytology; neurodevelopment; new technology; relating to nervous system; repaired; repository; research study; restoration; stem; tool

Purpose and Objectives Genetic and environmentally-induced developmental disorders can adversely affect central nervous system (CMS) development and function. The mechanisms of pathogenesis and neural repair as well as the translational research that will lead to the development of therapeutic treatments for regeneration of the damaged CNS have been increasingly the subject of intense study by researchers, including the members of this Center. To match the complexity of these problems one needs a multidisciplinary approach. Thus, it was to be useful to our neuroscientists to come to a single integrated neuroscience core where they could be advised by a team of expert faculty and staff of the proper approach, technique and equipment to use, to investigate their neuroscience research problems. To achieve this objective 3 cores were merged (Cell Biology, Cytology and Imaging). Expensive state-of-the-art instruments like the Zeiss Laser Scanning Confocal Microscope LSM 510 META, and the Leica Laser Cell Capture Microdissection system were purchased. Additional faculty were recruited to offer broad neuroscience expertise which together with contributions provided by other MRRC and Campus Cores, would satisfactorily address the vast needs of projects listed in the cores user table. Here the rationale for the services provided will be presented. The Neuroscience and Imaging Core provides expertise, services and equipment to support cellular and molecular neuroscience research: ¿ Stem Cell/ Cell Culture/Cell Transplant ¿ Zebrafish Facility ¿ Neurocytology ¿ Immunocytochemistry ¿ In Situ Hybridization ¿ Confocal Microscopy (Zeiss 510 META) ¿ Laser Cell Capture from tissue slices and cell cultures First, the in vitro/in vivo approaches of cell culture and cell transplantation will be discussed. The discovery that the adult CNS possesses the potential to regenerate via multipotent stem cells, has given rise to increasing interest on studying neural stem cells either to understand pathogenesis in neurodegenerative diseases in animal models or as potential therapeutic tools for degenerative diseases. The purpose of this core is to fulfill the need of incorporating new technology aimed at the study of the restoration of CNS function. The Core combines various specialized domains, including the culture of specific neural cells, neural stem and ES cells, their propagation, proper differentiation, identification and their use for neural transplantation. These approaches have proven to be promising in the field of CNS repair. The core integrates the state-of-the-art methodology on the preparation of embryonic stem cells (ES) and neural stem cells, as well as neuronal and glial cell cultures. Cell care and their proper identification and selection are of the essence to insure reproducible data. The selection of adequate tools such as live fluorescent labeling of cells to be used in transplant studies determines the long-term outcome of this type of studies. Investigators will be advised on the design of transplant studies and characterization of grafted cells. Zebrafish have become an important vertebrate animal model, not only for investigation of neural development but also for studies of behavior, neurodegenerative, metabolic and other disease processes, including cancer. Developmental studies in particular capitalize on this model, because of the transparency of embryos, enabling visualization of specific populations of neurons and their axons using green fluorescence-tagged transgenes, and the ability to do high throughput genetic screens. Moreover, the sequencings of zebrafish transcriptomes and the genome are near completion. A large set of mutants that affect nervous system development are already available. As such, there is a high level of interest in the Zebrafish model by members of the MRRC, as well as the overall biomedical research community at UCLA. In fact, a large centralized zebrafish facility in the Life Sciences building at UCLA is currently in the planning. Such a facility is needed to accommodate UCLA investigators who currently use, or expect to use this model, and for the recruitment of new faculty. The MRRC has enthusiastically supported this proposal, which should handle much of the space needs for large screens and the holding of major zebrafish stocks. However, much of the experimental work will need to be done either in individual laboratories, or more efficiently, in a common facility in each building shared by the investigators who use the model. Use of a common facility will be much more cost- and time-efficient because it will circumvent the need for each laboratory to learn the methods and provide routine care of fish, set up breeding, etc. It will also free up space in individual laboratories for other projects. To this end, we have dedicated a room for this purpose in the new Neuroscience Research building that will house most MRRC investigators. This room is approximately 350 ft2, and is capable of holding 3-5 racks of fish. This room will be managed by Dr. James A. Waschek with the help of Paul Zhao, SRA. Dr. Waschek recently returned from nine months of sabbatical leave at the laboratory or Dr. Stephen Ekker, at the University of Minnesota, who has used the zebrafish model for several years. Under Waschek's supervision, the technician will maintain all lines of fish, set up breedings, and provide embryos for individual investigators. The research associate will also teach embryo injection techniques to individual investigators.

目的和目标 遗传和环境引起的发育障碍会对 中枢神经系统（CMS）的发育和功能。发病机制和机制。 神经修复以及转化研究将导致发展 受损中枢神经系统再生的治疗已越来越成为主题 研究人员（包括该中心的成员）进行了大量的研究，以适应复杂性。 解决这些问题需要一种多学科的方法，因此，它对我们很有用。 神经科学家来到一个单一的综合神经科学核心，在那里他们可以得到建议 由专家教师和工作人员组成的团队使用适当的方法、技术和设备， 为了实现这一目标，研究他们的神经科学研究问题。 合并（细胞生物学、细胞学和成像）。 蔡司激光扫描共焦显微镜 LSM 510 META 和徕卡激光细胞捕获 购买了更多的教师来提供广泛的显微解剖系统。 神经科学专业知识以及其他 MRRC 和校园提供的贡献 核心，将令人满意地满足核心用户表中列出的项目的巨大需求。 这里将介绍所提供服务的理由。 神经科学和影像核心提供专业知识、服务和设备 支持细胞和分子神经科学研究： ¿干细胞/细胞培养/细胞移植 ¿斑马鱼设施 ¿神经细胞学 ¿免疫细胞化学 ¿原位杂交 ¿共焦显微镜（蔡司 510 META） ¿从组织切片和细胞培养物中进行激光细胞捕获 首先，细胞培养和细胞移植的体外/体内方法将是 讨论了成人中枢神经系统具有多能再生潜力的发现。 干细胞，引起了人们对研究神经干细胞越来越感兴趣 动物模型中神经退行性疾病的发病机制或潜在的 该核心的目的是满足退行性疾病的治疗工具。 结合旨在研究中枢神经系统功能恢复的新技术。 结合了各种专业领域，包括特定神经细胞的培养、神经干细胞 和 ES 细胞，它们的增殖、正确分化、鉴定及其在神经细胞中的用途 这些方法已被证明在中枢神经系统修复领域具有广阔的前景。 core 整合了最先进的胚胎干细胞 (ES) 制备方法 和神经干细胞，以及神经细胞和神经胶质细胞培养及其适当的培养。 识别和选择对于确保可重复的数据至关重要。 足够的工具，例如用于移植研究的细胞活荧光标记 决定此类研究的长期结果。 移植研究的设计和移植细胞的表征。 斑马鱼已成为重要的脊椎动物模型，不仅用于研究 神经发育的研究，也用于行为、神经退行性、代谢和其他方面的研究 疾病过程，包括癌症的发展研究尤其利用了这一点。 模型，由于胚胎的透明性，使得特定群体的可视化成为可能 使用绿色荧光标记的转基因的神经元及其轴突，以及执行高任务的能力 此外，斑马鱼转录组的测序和 基因组已接近完成，影响神经系统发育的一大组突变体。 因此，人们对斑马鱼模型非常感兴趣。 MRRC 的成员以及加州大学洛杉矶分校的整个生物医学研究界。 加州大学洛杉矶分校生命科学大楼内的一个大型集中斑马鱼设施目前位于 需要这样的设施来容纳目前使用的加州大学洛杉矶分校的调查人员，或 MRRC 期望使用这种模式，并且对新教师的招聘充满热情。 支持该提案，该提案应能满足大屏幕的大部分空间需求 然而，大部分实验工作都需要进行。 可以在单独的实验室中完成，或者更有效地在每栋大楼的公共设施中完成 由使用该模型的调查人员共享的信息将更多。 具有成本效益和时间效益，因为它将避免每个实验室学习 方法并提供鱼类的日常护理、建立养殖等。它还将释放空间 为此，我们专门为其他项目提供了一个房间。 在新的神经科学研究大楼内，大多数 MRRC 研究人员将居住在这个房间里。 面积约 350 平方英尺，可容纳 3-5 架鱼。 作者：James A. Waschek 博士，在 SRA 赵保罗的帮助下，Waschek 博士最近回来了。 从在实验室或斯蒂芬·埃克博士在大学的九个月休假中 明尼苏达州在瓦什切克的监督下使用斑马鱼模型已有多年。 技术人员将维护所有鱼系、进行繁殖并为个体提供胚胎 研究助理还将向个人研究人员教授胚胎注射技术。 调查人员。