Designer Retinal Circuits: Interfacing optical tweezers with an electronic device

设计师视网膜电路：光镊与电子设备的连接

基本信息

批准号：
7585225
负责人：
ELLEN S TOWNES-ANDERSON
金额：
$ 15.81万
依托单位：
UNIV OF MED/DENT OF NJ-NJ MEDICAL SCHOOL
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-04-01 至 2012-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7585225
关键词：
Action Potentials Address Adult Amacrine Cells Animal Model Area Biological Models Biology Brain Cell Adhesion Cell Communication Cell Culture Techniques Cell Survival Cell physiology Cell surface Cells Collaborations Cultured Cells Development Devices Dyes Electrodes Electronics Environment Eye Ganglia Glass Goals Growth Health Sciences Human Image In Vitro Injury Institutes Life Light Limb structure Membrane Potentials Microelectrodes Modeling Monitor Morphology Myxoid cyst Neural Retina Neuraxis Neurites Neurons New Jersey Organ Photoreceptors Play Population Positioning Attribute Preparation Prosthesis Recycling Retina Retinal Retinal Cone Retinal Diseases Role Specific qualifier value Sterility Surface Synapses Synaptic Vesicles System Techniques Technology Testing Time Tissues Transplantation Varicosity Vertebrate Photoreceptors Vision cell motility cell type design efficacy testing ganglion cell immunocytochemistry in vivo interest laser tweezer novel prototype reconstruction relating to nervous system repaired research study response retina transplantation retinal rods synaptogenesis voltage

项目摘要

DESCRIPTION (provided by applicant): Our goals are to examine the development and reconstruction of retinal circuits 1) that must form after retinal transplantation if some vision is to be restored, 2) that occur in the degenerate retina as the retina is remodeled, and 3) that function in the normal retina. We propose to do this by combining the technologies of optical tweezers with microelectrode arrays on glass chips. Chips will be designed to have non-adherent and adherent zones for cell movement and cell culture respectively. Using the optical tweezers, isolated adult retinal cells that are identified by their distinctive morphologies will be micromanipulated onto electrodes and allowed to form cell-cell contacts. Synaptic development will be monitored by immunocytochemistry and synaptic vesicle recycling dyes. Synaptic activity will be tested by electrode stimulation and imaging of non- toxic voltage-sensitive dyes for bipolar and some amacrine cells, and by recording electrodes for the spiking and action potentials of amacrine and ganglion cells. The use of the tweezers allows precise control over the circuits that are built. The ability to design and then functionally test specific nerve cell interactions over time will provide a system with an unprecedented level of experimental control.

描述（由申请人提供）：我们的目标是检查视网膜电路的开发和重建1）如果要恢复某些愿景，则必须在视网膜移植后形成，2）随着视网膜的重塑，并在退化视网膜中发生，并且3）在正常视网膜中发挥作用。我们建议通过将光学镊子的技术与玻璃芯片上的微电极阵列相结合来做到这一点。芯片将被设计为分别具有用于细胞运动和细胞培养的非粘附和粘附区域。使用光学镊子，将其独特形态鉴定出的孤立成年视网膜细胞将被微观掌握到电极上，并允许形成细胞细胞接触。突触发育将通过免疫细胞化学和突触囊泡回收染料来监测。突触活性将通过电极刺激和对双极敏感性染料的电极刺激和对双极敏感性染料的成像进行测试，并通过记录电极和神经节细胞的尖峰和动作电位的电极。镊子的使用允许精确控制所构建的电路。随着时间的推移，设计并在功能上测试特定神经细胞相互作用的能力将提供一个前所未有的实验控制水平的系统。