Bioreagents & Resources Core

生物试剂

基本信息

批准号：
10713943
负责人：
JOSEPH EDWARD WILLIS
金额：
$ 18.6万
依托单位：
CASE WESTERN RESERVE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-09-20 至 2028-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10713943
关键词：
Address Animals Archives Barrett Esophagus Barrett&apos s carcinogenesis Basic Science Biological Assay Blood Blood specimen Body Fluids Cell Line Clinical Clinical Research Collaborations Collection Color Complex Comprehensive Cancer Center Confidentiality of Patient Information Consultations Custom DNA DNA Library Development Diagnostic Dissection Dysplasia EPHB2 gene Ensure Epithelium Esophageal Adenocarcinoma Esophageal Neoplasms Esophageal Tissue Esophageal injury Esophagectomy Esophagus Faculty Family Family suidae Formalin Freezing Fresh Tissue Funding Genomics Gland Goals High grade dysplasia Histology Human Immunofluorescence Immunologic Immunohistochemistry In Situ Hybridization Inherited Institutional Review Boards Investigation Knock-in Knockout Mice Link Medical Records Metaplasia Microdissection Modeling Molecular Morphology Mus Outcome Paraffin Embedding Pathologist Pathology Pathway interactions Patients Play Predisposing Factor Predisposition Preparation Procedures Program Research Project Grants Quality Control RNA Reagent Records Research Personnel Research Project Grants Resource Sharing Resources Role Sampling Schedule Services Signal Transduction Site Specimen Submucosa Susceptibility Gene Technology Test Result Tissue Embedding Tissue Microarray Tissue Procurements Tissue Sample Tissues Translational Research United States National Institutes of Health Work biobank blood fractionation cancer prevention carcinogenesis central database design esophageal carcinogenesis experience follow-up gastrointestinal human tissue injury and repair insight laser capture microdissection meetings mouse model novel novel strategies programs quality assurance repository research study screening single cell sequencing skills success technology/technique tissue archive tissue processing tissue resource transcriptomics wound healing

项目摘要

PROJECT SUMMARY The Biospecimen and Reagents Core [BRC] provides high quality annotated specimens and expert pathology consultation to enhance the P01 projects. The BR&C CWRU and Duke components have long track records of supporting multi-investigator basic, translational and clinical research relating to multiple aspects of the Barrett's Esophagus [BE] and BE- neoplasia. The BRC is co-lead by two nationally recognized gastrointestinal pathologists with expertise in esophageal pathology who have well established track records in tissue-based translational research. The BRC co-leaders have worked together for many years and have longstanding collaborations with the P01 teams. The BRC components have differencing and complementary resources. Both the CWRU and the Duke components have large bioarchives which have been used to support multiple large scale NIH funded projects for over 20+ years. The BR&C has tissue and blood biospecimens from over 6,200 patients suitable for this P01. The BR&C leverages the Case CCC Shared Resources, the UH Pathology Translational Research Core and the Duke BioRepository & Precision Pathology Center shared resource to maximize efficiencies, utilize their expertise and advanced technologies. For Project 1 the BRC was integral to the discovery of germline abnormalities of VSIG10L as a predisposing factor in some patients with Familial Barrett's Esophagus. The BRC worked with Prj 1 to define the knock in and knock out mouse models which form the basis of this project and identified a multilayer epithelium complex in Prj 1 murine models – recognized as a robust marker of BE changes and linked to BE in humans. For Project 2 the BRC and collaborators developed the novel approach of using pigs as a model for esophageal injury – including the study of esophageal submucosal glands [ESMGs] as a potential site of origin of BE. Inspired by this effort, Prj 2 and the BRC identified and provided sections of ESMGs during screening of multiple esophagectomies and then worked with Prj 2 to immunoprofile the ESMGs. The molecular mechanisms identified formed the basis for the project. For Project 3 the BRC supplied high quality tissue samples and immunohistochemistry expertise in the investigation of EPHB2 signaling in esophageal carcinogenesis used to define potential pathways of development of BE – results of which formed the basis for the project. The BRC, with Core C, will continue to provide support to the P01 investigators and will enhance their projects by supplying quality controlled esophagus tissues, human and animal pathology expertise and facilitate state of the art tissue diagnostics including immunohistochemistry, ISH, tissue microdissection, spatial transcriptomics and single cell sequencing.

项目概要生物样本和试剂核心 [BRC] 提供高质量的带注释样本和专家病理学 BR&C CWRU 和杜克大学组成部分拥有长期的合作记录。支持与多个方面相关的多研究者基础、转化和临床研究 Barrett 食管 [BE] 和 BE- 肿瘤 BRC 由两个国家认可的胃肠病学委员会共同领导。具有食管病理学专业知识的病理学家，在基于组织的研究中拥有良好的跟踪记录 BRC 联合领导者已合作多年并拥有悠久的历史。与 P01 团队的合作具有差异性和互补性。 CWRU 和杜克大学的组件都拥有大型生物档案，可用于支持多个项目 BR&C 拥有超过 20 年的 NIH 资助的大型项目的组织和血液生物样本。 6,200 名患者适合本 P01。 BR&C 利用案例 CCC 共享资源，即 UH 病理学。转化研究核心和杜克大学生物样本库和精密病理学中心共享资源最大限度地提高效率，利用他们的专业知识和先进技术。对于项目 1，BRC 对于发现 VSIG10L 种系异常（作为诱发因素）不可或缺。 BRC 与 Prj 1 合作定义了一些家族性巴雷特食管患者的因素。并敲除构成该项目基础的小鼠模型并鉴定出多层上皮复合体在 Prj 1 小鼠模型中——被认为是 BE 变化的有力标志，并与人类的 BE 相关。对于项目 2，BRC 和合作者开发了使用猪作为模型的新方法食管损伤 – 包括将食管粘膜下腺 [ESMG] 作为潜在起源部位的研究受这一努力的启发，Prj 2 和 BRC 在筛选过程中确定并提供了 ESMG 的部分。多次食管切除术，然后与 Prj 2 一起对 ESMG 进行免疫分析。确定的机制构成了该项目的基础。对于项目 3，BRC 提供了高质量的组织样本和免疫组织化学专业知识食管癌发生中 EPHB2 信号传导的研究用于确定潜在的途径 BE 的开发——其结果构成了该项目的基础。 BRC 与 Core C 将继续为 P01 研究人员提供支持，并加强他们的项目通过提供质量受控的食道组织、人类和动物病理学专业知识并促进状态先进的组织诊断，包括免疫组织化学、ISH、组织显微切割、空间转录组学和单细胞测序。