STRUCTURAL ANALYSIS OF THE RIBOSOMAL ENHANCESOME

核糖体增强体的结构分析

• 批准号: 7602754
• 负责人: MAIR E CHURCHILL
• 金额: $ 0.89万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-13 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7602754
• 关键词: Binding Proteins; Biochemical; Complex; Computer Retrieval of Information on Scientific Projects Database; DNA Structure; Data; Eukaryota; Eukaryotic Cell; Funding; Genetic Transcription; Grant; Institution; Methods; Nucleoproteins; Process; RNA Polymerase I; Regulation; Research; Research Personnel; Resolution; Resources; Ribosomal RNA; Source; Structure; Transcript; United States National Institutes of Health; X-Ray Crystallography; numb protein; rRNA Genes

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Transcription of ribosomal RNA genes in Eukaryotes requires the action of a number of proteins for initiation and elongation of rRNA transcripts. The Upstream Binding Protein (UBF) is a key regulator of this process. Not only does it facilitate the activation of the ribosomal RNA genes, but it is important for stabilizing the DNA structure of the rRNA genes. It is known that the first 44 kDa of UBF is required for formation of a nucleoprotein complex called the ribosomal enhancesome. In order to interpret the extensive biochemical data implicating UBF in regulation of RNA polymerase I transcription, it is essential to understand the structure of the UBF enhancesome at atomic resolution. We propose to determine the structural features of this complex using cryo-EM and hopefully X-ray crystallography methods.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 真核生物中核糖体RNA基因的转录需要许多蛋白质的作用，以启动和伸长rRNA转录本。 上游结合蛋白（UBF）是此过程的关键调节剂。 它不仅促进了核糖体RNA基因的激活，而且对于稳定RRNA基因的DNA结构很重要。众所周知，UBF的第一个44 kDa是形成称为核糖体增强体的核蛋白复合物所必需的。 为了解释涉及RNA聚合酶I转录调节UBF的广泛的生化数据，必须了解在原子分辨率下增强UBF的结构。我们建议使用冷冻EM和希望X射线晶体学方法确定该复合物的结构特征。