REGULATOR OF HOMOLOGOUS RECOMBINATION IN EUKARYOTIC CELLS

真核细胞同源重组的调节因子

• 批准号: 7602147
• 负责人: PAUL RUSSELL
• 金额: $ 0.62万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7602147
• 关键词: Cells; Complex; Computer Retrieval of Information on Scientific Projects Database; Eukaryota; Eukaryotic Cell; Fingers; Fission Yeast; Funding; Genetic Recombination; Grant; Homologous Gene; Human; Institution; Numbers; Pathway interactions; Proteins; Research; Research Personnel; Resources; Saccharomycetales; Source; Swimming; United States National Institutes of Health; XRCC2 gene; Yeasts; helicase; homologous recombination; mutant; novel; paralogous gene

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Rad52-dependent homologous recombination (HR) is regulated by the antirecombinase activities of Srs2 and Rqh1/Sgs1 DNA helicases in fission yeast and budding yeast. Functional analysis of Srs2 in Schizosaccharomyces pombe led us to the discovery of Sws1, a novel HR protein with a SWIM-type Zn finger. Inactivation of Sws1 suppresses the genotoxic sensitivity of srs2 and rqh1 mutants and rescues the inviability of srs2 rqh1 cells. Sws1 functions at an early step of recombination in a pro-recombinogenic complex with Rlp1 and Rdl1, two RecA-like proteins that are most closely related to the human Rad51 paralogs XRCC2 and RAD51D, respectively. This finding indicates that the XRCC2?RAD51D complex is conserved in lower eukaryotes. A SWS1 homolog exists in human cells. It associates with RAD51D and ablating its expression reduces the number of RAD51 foci. These studies unveil a conserved pathway for the initiation and control of HR in eukaryotic cells.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 RAD52依赖性同源重组（HR）受裂变酵母和萌芽酵母中SRS2和RQH1/SGS1 DNA解旋酶的抗聚合酶活性调节。 SRS2在schizosaccharomyces pombe中的功能分析使我们发现了SWS1，这是一种新型的HR蛋白，带有游泳型Zn手指。 SWS1的失活抑制了SRS2和RQH1突变体的遗传毒性敏感性，并挽救了SRS2 RQH1细胞的不可侵袭性。 SWS1在与RLP1和RDL1的亲重组复合复合物中重组的早期作用，这是两个与人Rad51旁系同源物XRCC2和RAD51D最紧密相关的RECA样蛋白。这一发现表明XRCC2？RAD51D复合物在较低的真核生物中是保守的。 SWS1同源物存在于人类细胞中。它与rad51d相关联并消融其表达可减少RAD51焦点的数量。这些研究揭示了真核细胞中HR的启动和控制的保守途径。