SCREENING FOR BXI1P (BAX INHIBITOR-1) INTERACTING PROTEINS

筛选 BXI1P（BAX 抑制剂-1）相互作用蛋白

• 批准号: 7602136
• 负责人: NICANOR AUSTRIACO
• 金额: $ 1.04万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7602136
• 关键词: Animals; Apoptosis; Apoptotic; Cell Death; Computer Retrieval of Information on Scientific Projects Database; Drosophila melanogaster; Funding; Grant; Homo sapiens; Homologous Gene; House mice; Human; Institution; Knowledge; Mouse-ear Cress; Organism; Protein Family; Proteins; Range; Research; Research Personnel; Resources; Saccharomyces cerevisiae; Saccharomycetales; Screening procedure; Source; United States National Institutes of Health; Yeasts; inhibitor/antagonist; member; plant fungi; yeast two hybrid system

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Bax Inhibitor-1 (BI-1) was first identified by its ability to block Bax-induced apoptotic cell death in the budding yeast, Saccharomyces cerevisiae. It is one member of the Bcl-2 family of proteins that regulates programmed cell death. Intriguingly, BI-1 is one of a few cell death inhibitors found in a wide range of fungi, plant, and animal species including Saccharomyces cerevisiae, Drosophila melanogaster, Arabidopsis thaliana, Mus musculus, and Homo sapiens. This suggests that it is an ancient protein. We are characterizing the yeast homolog of BI-1, which we are calling BXI1. Using a two-hybrid screen, we would like to identify proteins that interact with Bxi1p. With knowledge of the function of BXI1 in yeast we hope to better understand the function of Bax Inhibitor-1 in higher organisms including human beings.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 BAX抑制剂1（BI-1）首先是通过阻断酿酒酵母酿酒酵母中巴克斯诱导的凋亡细胞死亡的能力来识别的。 它是调节程序性细胞死亡的BCL-2蛋白质家族的成员。 有趣的是，BI-1是少数几种细胞死亡抑制剂之一，包括酿酒酵母，果蝇，果蝇Melanogaster，拟南芥，穆斯马库鲁斯，穆斯库鲁斯和同性恋者在内的多种真菌，植物和动物物种中发现的一种细胞死亡抑制剂之一。这表明它是一种古老的蛋白质。我们正在表征BI-1的酵母同源物，我们称之为BXI1。 使用两个杂交屏幕，我们想识别与BXI1P相互作用的蛋白质。 借助BXI1在酵母中的功能，我们希望更好地了解BAX抑制剂1在包括人类在内的较高生物体中的功能。