GENETIC CHARACTERIZATION OF TWO GENES INVOLVED IN YEAST PROGRAMMED CELL DEATH

参与酵母程序性细胞死亡的两个基因的遗传特征

• 批准号: 8167608
• 负责人: NICANOR AUSTRIACO
• 金额: $ 2.54万
• 依托单位: UNIVERSITY OF RHODE ISLAND
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8167608
• 关键词: Apoptosis; Apoptotic; Biological Models; Biological Process; Cell Wall; Cells; Complex; Computer Retrieval of Information on Scientific Projects Database; Defense Mechanisms; Funding; Genes; Genetic; Grant; Human; Institution; Laboratories; Lead; Link; Malignant Neoplasms; Modeling; Molecular; Organism; Pathway interactions; Phenotype; Physiological Processes; Process; Proteins; Research; Research Personnel; Resources; Saccharomyces cerevisiae; Saccharomycetales; Source; System; United States National Institutes of Health; Work; Yeasts; college; genetic analysis; insight; mutant; response; tumor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Programmed cell death (PCD) is both an important physiological process and a significant anti-tumor defense mechanism in multicellular organisms. Some have even called the ability to evade programmed cell death a "hallmark of cancer". The budding yeast, Saccharomyces cerevisiae, has served as a useful model for complex physiological processes of metazoan cells including PCD. Much work has gone into attempting to describe the molecular mechanisms that drive programmed cell death. For the past four years, my laboratory at Providence College has studied UTH1 and BXI1, two genes linked with programmed cell death in yeast. We have generated mutants lacking UTH1 and BXI1 and have shown that they have phenotypes linking them to the cell wall and the unfolded protein response respectively. This proposal outlines genetic strategies to identify the molecular pathways involved in UTH1 and BXI1 function. It will exploit the primary advantage of the yeast system over its mammalian counterpart as a model system for programmed cell death: Yeast cells are amenable to genetic analysis that allows investigators to identify rapidly molecular pathways underlying a biological process. The genetic strategies described in this proposal will seek to clarify the genetic relationships between UTH1, BXI1, and other PCD genes in yeast to identify the molecular pathways that regulate programmed cell death in this organism. Given the remarkable conservation of the apoptotic pathways across diverse species, this analysis should lead to further insights into the analogous processes in higher organisms including human beings.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 程序性细胞死亡（PCD）既是多细胞生物中重要的生理过程，也是重要的抗肿瘤防御机制。一些人甚至称逃避程序性细胞死亡的能力为“癌症的标志”。 萌芽的酵母酿酒酵母已成为复杂生理过程的有用模型，包括PCD。尝试描述驱动编程细胞死亡的分子机制进行了许多工作。 在过去的四年中，我在普罗维登斯学院（Providence College）的实验室研究了UTH1和BXI1，这是两个与酵母中编程细胞死亡有关的基因。 我们已经产生了缺乏UTH1和BXI1的突变体，并表明它们具有将它们与细胞壁和展开的蛋白质反应联系起来的表型。该建议概述了识别UTH1和BXI1功能涉及的分子途径的遗传策略。 它将利用酵母菌系统比其哺乳动物对应物的主要优势作为程序性细胞死亡的模型系统：酵母细胞可与遗传分析相吻合，这使研究人员能够识别生物学过程的迅速分子途径。 该提案中描述的遗传策略将旨在阐明酵母中UTH1，BXI1和其他PCD基因之间的遗传关系，以鉴定调节该生物体中编程细胞死亡的分子途径。 鉴于对各种物种的凋亡途径的显着保护，该分析应进一步了解包括人类在内的高等生物的类似过程。