The mode-of-action for pheromone-induced paralysis in Pristionchus pacificus

信息素引起的太平洋原虫麻痹的作用方式

• 批准号: 9978066
• 负责人: Ray L Hong
• 金额: $ 10.88万
• 依托单位: CALIFORNIA STATE UNIVERSITY NORTHRIDGE
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-01 至 2021-08-01
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9978066
• 关键词: Acetonitriles; Alleles; Animals; Anthelmintics; Binding Proteins; CRISPR/Cas technology; Caenorhabditis elegans; Candidate Disease Gene; Cells; Development; Developmental Process; Drug usage; Embryo; Evolution; Exposure to; Gene Expression; Genes; Genetic; Genetic Screening; Haemonchus; Head; Hour; Human; Hypersensitivity; Insecta; Larva; Lead; Lesion; Ligands; Lipid Binding; Livestock; Maps; Mediating; Modeling; Mutagenesis; Nematoda; Nematode infections; Neuroglia; Neurons; Order Coleoptera; Paralysed; Parasites; Parasitic nematode; Phenocopy; Phenotype; Pheromone; Pheromone Receptors; Phylogenetic Analysis; Physiological; Population; Preventive Medicine; Reporter; Research Support; Resistance; Role; Sex Attractants; Signal Pathway; Signal Transduction; Transforming Growth Factor beta; Transforming Growth Factors; Translating; Work; comparative; forward genetics; genetic approach; genome editing; improved; loss of function; mutant; new therapeutic target; novel; pet animal; receptor; resistance gene; reverse genetics

Project Summary Parasitic nematodes have long harmed crops, pets, livestock, and humans. Wide usage of current broad-spectrum anthelmintics as preventive medicine has led to emerging nematode resistance against these compounds in many human populations, prompting the exploration of compounds with novel genetic targets. Our discovery that an insect pheromone can act both as an attractant as well as an anthelmintic represents a unique opportunity to define a novel drug target. Our proposal is to identify the mode-of-action for this pheromone using a lipid- binding protein as the genetic entry point. We propose three independent strategies involving candidate gene expression and functional analysis, along with forward genetic screens to identify the pheromone receptor and other components of the signaling pathway.

项目概要 寄生线虫长期以来一直危害农作物、宠物、牲畜和人类。宽的 使用当前的广谱驱虫药作为预防药物已导致 许多人类线虫对这些化合物产生了抗药性 人群，促进了对具有新遗传靶标的化合物的探索。 我们发现昆虫信息素既可以作为引诱剂，也可以作为诱食剂 驱虫药代表了定义新药物靶点的独特机会。 我们的建议是使用脂质来确定这种信息素的作用模式 结合蛋白作为遗传切入点。我们建议三个独立的 涉及候选基因表达和功能分析的策略，以及 通过正向遗传筛选来识别信息素受体和其他 信号通路的组成部分。

项目成果

Interspecific comparison of sensitivity to paralytic compounds.

对麻痹化合物敏感性的种间比较。

• 发表时间: 2019-12-17
• 作者: Le, Vivian Vy;Sanchez, Bryan;Hong, Ray
• 通讯作者: Hong, Ray

A species-specific nematocide that results in terminal embryogenesis.

一种导致终末胚胎发生的物种特异性杀线虫剂。

• 发表时间: 2017-09-15
• 作者: Renahan, Tess;Hong, Ray L
• 通讯作者: Hong, Ray L

The bacterial community of entomophilic nematodes and host beetles.

昆虫线虫和宿主甲虫的细菌群落。

• 发表时间: 2016-05
• 影响因子: 4.9
• 作者: Koneru, Sneha L;Salinas, Heilly;Flores, Gilberto E;Hong, Ray L
• 通讯作者: Hong, Ray L

Antileishmanial activity of a new chloroquine analog in an animal model of Leishmania panamensis infection.

新型氯喹类似物在帕纳门利什曼原虫感染动物模型中的抗利什曼活性。

• 发表时间: 2020
• 作者: Herrera, Lizzi;Llanes, Alejandro;Álvarez, Jennifer;Degracia, Kissy;Restrepo, Carlos M;Rivera, Rene;Stephens, David E;Dang, Hang T;Larionov, Oleg V;Lleonart, Ricardo;Fernández, Patricia L
• 通讯作者: Fernández, Patricia L