Cell Biology and Bioimaging Core

细胞生物学和生物成像核心

基本信息

批准号：
9978086
负责人：
David H Burk
金额：
$ 15.57万
依托单位：
LSU PENNINGTON BIOMEDICAL RESEARCH CTR
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-08-01 至 2022-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9978086
关键词：
3-Dimensional Adipose tissue Adopted Advisory Committees Algorithmic Software Animal Model Antibodies Biomedical Research Cell Separation Cells Cellular biology Centers of Research Excellence Charge Core Facility Data Data Analyses Development Diabetes Mellitus Diabetic Diet Ectopic Expression Energy Transfer Equipment Extramural Activities Faculty Flow Cytometry Fluorescence Fluorescence Recovery After Photobleaching Freezing Funding Gene Expression Profile Generations Genomics Histology Image Image Analysis Image Enhancement Imaging Techniques Institution Investments Lasers Louisiana Measures Mentors Messenger RNA Metabolic Diseases Metabolic syndrome Methodology Methods Microdissection Microscope Mission Modeling Modernization Nutritional Obesity Organelles Pancreas Paraffin Pathology Phase Phenotype Positioning Attribute Preparation Proteins Research Research Personnel Research Support S Phase Scanning Self Assessment Services Slide Specimen Stains Techniques Technology Tissue Stains Tissues Training Transcript Transgenic Organisms Work base bioimaging confocal imaging cost cost effective fluorescence imaging genetic manipulation imaging modality instrumentation live cell imaging mRNA Expression novel novel strategies programs quantitative imaging recruit success tissue processing

项目摘要

Cutting edge research in the field of metabolic disease requires access to modern imaging, flow cytometry, and specimen preparation methodology. The COBRE Cell Biology and Bioimaging Core (CBB Core) is an integrative component within the proposed Phase III cores – Transgenics, Genomics, and Cell Biology and Bioimaging. The CBB Core provides investigators access to the instrumentation, training, and expertise needed to conduct cellular and tissue level phenotyping of nutritionally-modulated or genetically-manipulated models of obesity, diabetes, or other pathologies of metabolic syndrome. The CBB Core enables both junior and senior level faculty to explore novel approaches that include quantitative histology and tissue specific transcript profiling via laser microdissection to increase their competitiveness in an era of shrinking federal funding. The Core's current capabilities include: widefield and confocal imaging of fixed and live specimens, total internal reflection fluorescence (TIRF), fluorescent resonant energy transfer (FRET), fluorescence recovery after photobleaching (FRAP), whole slide scanning, analytical and preparative flow cytometry, laser microdissection, and histology services that encompass tissue processing, embedding, sectioning, and staining – routine, special, and immunohistochemical. The Core continually adapts to the changing needs of the COBRE investigators through frequent self-assessments and oversight by the External Advisory Committee and the proposed Internal Steering Committee headed by the Program Coordinator, Dr. Claude Bouchard. The sustainability of the Core is likely due to the high level of commitment by the PBRC Administration as well as increases in the number of COBRE investigators that will receive extramural funding. The CBB Core aims are: 1) To provide cost effective specimen preparation and produce high quality bioimaging and flow cytometry data; 2) To provide access, training, and assistance with CBB Core platforms, in concert with expertise in application of the requisite software algorithms needed to produce state-of-the art data analysis, interpretation, and presentation; 3) To promote development new techniques, technologies, and experimental approaches needed to enhance COBRE faculty competitiveness for extramural funding.

代谢疾病领域的前沿研究需要现代成像、血流细胞计数法和样本制备方法。核心（CBB 核心）是拟议的第三阶段核心中的一个集成组件 – CBB 核心为研究人员提供转基因、基因组学、细胞生物学和生物成像。获得进行细胞和组织水平所需的仪器、培训和专业知识肥胖、糖尿病的营养调节或基因操纵模型的表型分析 CBB 核心支持初级和高级水平。教师探索包括定量组织学和组织特异性在内的新方法通过激光显微切割进行转录谱分析，以提高其在这个时代的竞争力联邦资金不断减少。Core 目前的能力包括：宽场和共聚焦。固定样本和活体样本成像、全内反射荧光 (TIRF)、荧光共振能量转移 (FRET)、光漂白后荧光恢复 (FRAP)、整体载玻片扫描、分析和制备型流式细胞术、激光显微切割和组织学服务包括组织处理、包埋、切片和染色——常规、特殊的和免疫组织化学的核心不断适应不断变化的需求。 COBRE 调查人员通过外部咨询的频繁自我评估和监督委员会和拟议的由计划协调员领导的内部指导委员会，克劳德·布沙尔（Claude Bouchard）博士。核心的可持续性可能归功于高水平的承诺。 PBRC 管理机构的监管以及 COBRE 调查员数量的增加，获得外部资助 CBB 的核心目标是： 1) 提供具有成本效益的样本。准备和产生高质量的生物成像和流式细胞术数据；2) 提供访问、培训和 CBB 核心平台协助，并结合应用方面的专业知识产生最先进的数据分析、解释、 3）促进新技术、新工艺和实验的发展；提高 COBRE 教师在校外资助方面的竞争力所需的方法。