TOMOGRAPHY OF SKELETAL MUSCLE TRIADIC JUNCTION

骨骼肌三联结断层扫描

• 批准号: 7598376
• 负责人: CLARA FRANZINI-ARMSTRONG
• 金额: $ 1.15万
• 依托单位: WADSWORTH CENTER
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-02-01 至 2008-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7598376
• 关键词: Calcium; Calcium Channel; Computer Retrieval of Information on Scientific Projects Database; Electron Microscope; Freezing; Frozen Sections; Funding; Grant; Institution; L-Type Calcium Channels; Membrane; Muscle Fibers; Research; Research Personnel; Resources; Skeletal Muscle; Source; Surface; United States National Institutes of Health; abstracting; instrumentation; pressure; tomography; voltage

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. ABSTRACT: The aim is to reconstruct the specific association between two calcium channels in skeletal muscle (the L type calcium channel of DHPR of the surface membrane/T tubules) and the calcium release channel of the SR. This can be done by tomography of the SR/T tubule junctions in an intact muscle fiber, and requires specific instrumentation available at the Albany Resource: A high pressure rapid freezing apparatus, an ultramicrotome for frozen sections, and intermediate high voltage cryo-electron microscope with high tilt angle.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 抽象的： 目的是重建骨骼肌（表面膜/T小管的DHPR的L型钙通道）和SR的钙释放通道之间的两个钙通道之间的特定关联。 这可以通过完整的肌肉纤维中SR/T小管连接的断层摄影术来完成，并且需要在奥尔巴尼资源上提供特定的仪器：高压快速冻结设备，用于冷冻切片的超级微观机，以及中间的高电压冷冻冷冻量显微镜，具有高倾斜角度。