TOMOGRAPHY OF SKELETAL MUSCLE TRIADIC JUNCTION

骨骼肌三联结断层扫描

• 批准号: 8172283
• 负责人: CLARA FRANZINI-ARMSTRONG
• 金额: $ 0.54万
• 依托单位: WADSWORTH CENTER
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-07 至 2011-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172283
• 关键词: Calcium; Computer Retrieval of Information on Scientific Projects Database; Data Analyses; Dihydropyridine Receptors; Freezing; Funding; Grant; Imagery; In Situ; Institution; Learning; Mediating; Methods; Muscle; Muscle Fibers; Pennsylvania; Plastic Embedding; Plastics; Proteins; Reporting; Research; Research Personnel; Resources; Ryanodine Receptor Calcium Release Channel; Ryanodine Receptors; Skeletal Muscle; Source; Specimen; Tomogram; Triad Acrylic Resin; United States National Institutes of Health; Universities; Visit; Work; abstracting; experience; particle; pressure; reconstruction; sensor; tomography; voltage

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. ABSTRACT: We are extending our work on the ryanodine receptor in skeletal muscle to in-situ observation, with the hope of visualizing dihydropyridine receptors (the voltage sensors that mediate the electrical impulse and the release of calcium that causes contraction). In the single-particle ryanodine receptor work, and the work on isolated triad junctions, done so far in Dr. Wagenknecht's lab, the dihydropyridine receptors were either removed or disrupted during isolation. We are collaborating with Dr. Clara Franzini-Armstrong of the University of Pennsylvania, who is a leader in the study of the excitation-contraction apparatus. With the benefit of her long experience in preparing muscle tissue for optimal visualization of the triad junction in situ, we are developing methods for high-pressure freezing muscle tissue in the proper orientation for observation of the dihydropyridine receptors. In the previous reporting period, Dr. Franzini-Armstrong visited the Resource and presented a seminar to describe the project. She also worked with Chyongere Hsieh in preparing specimens for high-pressure freezing. Tomographic reconstructions were made from thin, conventionally-prepared plastic sections provided by Dr. Franzini-Armstrong. This was done in order to learn to recognize the ideal orientation of the muscle fiber for revealing the DHPR. Initially, we are studying tomographic reconstructions from plastic sections. We have so far made six tomograms of plastic-embedded material supplied to us by Dr. Franzini-Armstrong, and we are analyzing this data for evidence of dihydropyridine receptors or other proteins related to excitation-contraction

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 抽象的： 我们正在将骨骼肌中ryanodine受体的工作扩展到原位观察结果，希望能够可视化二氢吡啶受体（介导电脉冲的电压传感器和引起收缩的钙的释放）。 在迄今为止在Wagenknecht博士实验室进行的单粒子ryanodine受体工作以及孤立的三合会连接的工作，在分离过程中，二氢吡啶受体被去除或破坏。 我们正在与宾夕法尼亚大学的Clara Franzini-Armstrong博士合作，宾夕法尼亚大学是兴奋促进设备的研究领导者。 由于她在制备肌肉组织方面的悠久经验以最佳可视化三合会的原位，我们正在开发高压冻结肌肉组织的方法，以适当的方向观察二氢吡啶受体。 在上一个报告期间，Franzini-Armstrong博士访问了资源，并提出了一个研讨会来描述该项目。 她还与Chyongere Hsieh合作，准备了标本以进行高压冻结。 层析成像重建是由Franzini-Armstrong博士提供的薄塑料部分进行的。 这样做是为了学会识别肌肉纤维的理想取向以揭示DHPR。 最初，我们正在研究塑料部分的层析成像重建。 到目前为止