SPECTRAL ANALYSIS FLOW CYTOMETRY

光谱分析流式细胞术

• 批准号: 7598385
• 负责人: JAMES H JETT
• 金额: $ 14.67万
• 依托单位: LOS ALAMOS NAT SECTY-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-30 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7598385
• 关键词: Back; Computer Retrieval of Information on Scientific Projects Database; Development; Evaluation; Flow Cytometry; Fluorescence; Funding; Goals; Grant; Institution; Lasers; Light; Light Cell; Outcome; Research; Research Personnel; Resources; Source; System; Testing; United States National Institutes of Health; detector

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A spectral analysis capable flow cytometer is being developed. Currently, all flow cytometers separate fluorescence emissions by dichroic mirrors plus spectral filters. The goal of this development is to analyze the fluorescence emissions by recording with a line CCD array spectrally dispersed light. Preliminary calculations indicate that back thinned CCD arrays have sufficient sensitivity to detect spectrally dispersed light from cells that are in the laser beam for 100 microseconds. A back thinned detector evaluation kit has been obtained from Hamamatsu Corporation. It is being integrated into an existing flow cytometer for evaluation. Pending the outcome of the tests, we will purchase s CCD array for permanent integration into the flow system.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 一种具有光谱分析能力的流式细胞仪正在开发中。 目前，所有流式细胞仪均通过二向色镜和光谱滤光片分离荧光发射。 这项开发的目标是通过线阵 CCD 阵列记录光谱色散光来分析荧光发射。 初步计算表明，背薄式 CCD 阵列具有足够的灵敏度，可以检测激光束中 100 微秒的细胞发出的光谱色散光。 背薄探测器评估套件已从 Hamamatsu Corporation 获得。 它正在被集成到现有的流式细胞仪中进行评估。 在等待测试结果之前，我们将购买 CCD 阵列以永久集成到流系统中。