DETECTION OF ALZHEIMER'S DISEASE USING A POLYMER NANOWIRE-BASED BIOSENSOR

使用基于聚合物纳米线的生物传感器检测阿尔茨海默病

基本信息

批准号：
7596948
负责人：
Maksudul Alam
金额：
$ 19.95万
依托单位：
INNOSENSE, LLC
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-04-01 至 2010-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7596948
关键词：
Address Affect Alzheimer disease detection Alzheimer disease screening Alzheimer&apos s Disease American Amides Amyloid Antibodies Antigen Targeting Antigens Area Autopsy Binding Biochemistry Biocompatible Biological Markers Biosensor Boxing Brain Caliber California Caregivers Caring Characteristics Chemicals Clinical Dementia Deposition Detection Development Devices Diagnostic Disease Disease Marker Early Diagnosis Early treatment Elderly Electrical Resistance Electrodes Electronics Electroplating Ensure Exposure to Fertilization Film Germ Cells Goals Growth Human Individual Knowledge Laboratory Diagnosis Left Length Ligand Binding Ligands Link Measures Mediating Methods Microelectrodes Modeling Modification Molecular Molecular Biology Monitor Neurodegenerative Disorders Noise One-Step dentin bonding system Oxidation-Reduction Pathogenesis Pattern Peptides Performance Phase Polymers Principal Investigator Process Property Protocols documentation Quality of life Research Resistance Role Sampling Sea Urchins Senile Plaques Signal Transduction Silicon Solutions Specificity Surface Symptoms System Techniques Technology Temperature Time Universities Work antigen antibody binding artist base clinical Diagnosis computerized data processing cost cost effective covalent bond design detector disease diagnosis electrical property end of life experience improved innovation meetings monolayer monomer nano nanoscale nanowire plaque lesion polyaniline polymerization polypyrrole professor prototype response self assembly sensor solid state success tool

项目摘要

DESCRIPTION (provided by applicant): InnoSense LLC (ISL) submits this revised application with the intent of developing a cost-effective and highly sensitive polymer nanowire-based electronic biosensor (PNEBD) for the early screening of Alzheimer's disease (AD). This device is expected to have potentially significant implications in the early treatment and possible delay of debilitating symptoms associated with this disease. The Phase I project is focused on the development and verification of a high-throughput device based on silicon photolithography processes and template-free electrodeposition of functionalized conductive polymers between narrow-gap electrode junctions. Six tasks have been proposed to establish the Phase I feasibility of detecting amyloid-¿ derived diffusible ligand (ADDL) antigen, a biomarker for AD, using amyloid-¿ (A¿) oligomer specific antibody to ADDLs. Current evidence strongly suggests a central role for amyloid-¿ (A¿) in the pathogenesis of AD. At the present time there is no definite clinical diagnosis for AD other than autopsy. It has been demonstrated that ADDLs are present at significantly elevated levels in the autopsied brain samples of humans with AD. This association of ADDLs with AD suggests that the PNEBD device could provide a definitive molecular basis for the laboratory diagnosis of AD. For ensuring success, a highly skilled research team, including an expert on AD, has been assembled to execute the project. Development of ultrasensitive detection methods for early screening of Alzheimer's disease (AD) is an important issue in clinical disease diagnosis and mechanistic understanding of Alzheimer's disease (AD). AD is a progressive neurodegenerative disease for which there is neither a cure nor an early diagnostic device. It is the leading cause of dementia in people over age 65 affecting over four million Americans. Despite its utmost importance, no ultrasensitive technologies have been developed for clinical lab diagnostic of AD. InnoSense LLC aims to fill this void by developing a cost-effective and highly-sensitive polymer nanowire-based electronic biosensor device (PNEBD). This device will utilize electrochemically grown polymer nanowires at micro- to nanoscale patterned electrode junctions for detecting amyloid-¿ derived diffusible ligands (ADDLs). ADDL has been established as a biomarker for AD. The purpose of using an electrochemical method is to grow template-free, low-cost and high quality polymer nanowires on to the electrode junctions. This direct, one-step fabrication technique is scalable. This project will advance our knowledge on the detection of Alzheimer's disease.

描述（由申请人提供）：Innosense LLC（ISL）提交了恢复的申请，以开发了基于成本效率和敏感的聚合物电子生物传感器（PNEBD），以便对阿尔茨海默氏病（AD）进行早期筛查预计将在早期的触发下有效，并可能延迟与Tis Tis Tis Tis Tis Tis Tis Tis Tis Tis Tis Tis Tis Tis Tis Tis Tis Tis Tis Tis Tis Tis Tis tis tis tis tis有些欺骗的开发和基于硅齿设备的验证，这可能会延迟。光学通知过程A.窄间隙连接之间功能化导电聚合物的无模板电虫病。使用淀粉样蛋白 - 衍生的扩散配体（ADDL）抗原是AD的生物标志物（a。）对当前证据的低聚物特异性抗体。（a）在AD的AD发病机理中。为了确保将广告专家推出的AD诊断。对阿尔茨海默氏病的机械理解（AD）。 AD的诊断Innosense LLC旨在通过开发基于成本效益的高敏感聚合物纳米生物传感器设备（PNEBD）来填充TOIS。衍生的配体（ADDL）。