Green Tea Anticancer Mechanisms in Smokers

绿茶对吸烟者的抗癌机制

基本信息

批准号：
7665513
负责人：
Philip T Diaz
金额：
$ 13.5万
依托单位：
OHIO STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-08-01 至 2013-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7665513
关键词：
Acids Affect Animals Anti-Inflammatory Agents Anti-inflammatory Antioxidants Attention Biological Biological Assay Biological Markers Blood Bronchoalveolar Lavage Cell Culture Techniques Chemoprevention DNA Adducts DNA Methylation Data Defense Mechanisms Development Evaluation Flavonoids Food Generic Drugs Green tea Herb Human In Vitro Incidence Inflammatory Intake Intervention Intervention Studies Laboratories Lead Lesion Life Link Lung Malignant Neoplasms Malignant neoplasm of lung Measures Messenger RNA Molecular Nutritional Oncogenes Oral Oxidants Perception Phosphorylation Phytochemical Placebos Population Positioning Attribute Process Protein-Lysine 6-Oxidase Publications Research Research Personnel Rodent Sampling Site Smoker Stress Study Section Superoxides TNF gene Tea Testing Time Tissues Urine Work base cancer chemoprevention cancer prevention cancer risk cancer site design epidemiology study expectation experience human study improved innovation intervention effect minimally invasive neutrophil oxidant stress prevent response smoking cessation young adult

项目摘要

DESCRIPTION (provided by applicant): Studies in vitro, studies in rodents, and some epidemiology studies suggest cancer preventing mechanisms of phytochemicals in green tea, which can be called an herb or a food. Yet, not one green tea human intervention study exists for cancer incidence, nor does much intervention data exist for pre-cancer biomarkers. In fact, it hasn't even been shown that a human green tea intervention can even impact the various anti-cancer defense mechanisms attributed to green tea (which should be a prerequisite to major studies on cancer incidence or pre-cancer biomarkers). Therefore, a project is proposed to test the following hypothesis: in young adult smokers, 6 weeks of 6 cups of green tea/d produces changes in bronchoalveolar lavage (BAL) samples related to improved anti- lung cancer defense mechanisms. This does not suppose that green tea can prevent lung cancer in smokers better than smoking cessation. However, the pre-cancer stress in smokers provides a chance to see changes in cancer risk-related parameters in a short time. Moreover, this lung cancer-relevant population allows use of BAL, a minimally invasive sampling of a site of potential cancer onset. Project end points relate to defenses against cancer development, and have shown changes due to green tea or its flavonoids in animals and/or in vitro, and can be measured in BAL samples, and in most cases, have shown abnormal BAL values in smokers. End points include: DNA adducts, ERK 1/2 phosphorylation, lysyl oxidase activity, two endogenous antioxidant defense molecules; neutrophil superoxide release; and TNF mRNA. This PI has worked with BAL samples, has access to smokers and BAL sampling via a collaborator MD, has rare experience with the lysyl oxidase assay, and possesses relevant preliminary data. Positive results in this project can lead to studies on tea or tea flavonoid supplements which examine pre-cancer biomarkers (ie DNA methylation), and ultimately, incidence of lung and other cancers. Negative results will justify re-evaluation of how to study green tea for chemoprevention.Green tea may produce body effects that help prevent cancer, but human studies haven't fully shown this yet. This project looks for these effects in smokers using a wash sample from lungs, a site where cancer can occur in smokers. Although green tea may not prevent lung cancer in all smokers, this group is good to study since they can show changes in cancer relevant measures faster than other people

描述（由申请人提供）：体外研究、啮齿动物研究和一些流行病学研究表明，绿茶中的植物化学物质具有预防癌症的机制，绿茶可以称为草药或食物。然而，目前还没有一项关于癌症发病率的绿茶人类干预研究，也没有太多关于癌前生物标志物的干预数据。事实上，甚至没有证据表明人类绿茶干预甚至可以影响绿茶的各种抗癌防御机制（这应该是癌症发病率或癌前生物标志物重大研究的先决条件）。因此，提出了一个项目来检验以下假设：在年轻的成年吸烟者中，每天喝 6 杯绿茶 6 周会导致支气管肺泡灌洗 (BAL) 样本发生变化，这与抗肺癌防御机制的改善有关。这并不意味着绿茶比戒烟更能预防吸烟者的肺癌。然而，吸烟者的癌前压力提供了在短时间内观察癌症风险相关参数变化的机会。此外，这种肺癌相关人群允许使用 BAL，这是一种对潜在癌症发病部位进行微创取样的方法。项目终点与预防癌症发展相关，并在动物和/或体外显示出绿茶或其黄酮类化合物引起的变化，并且可以在 BAL 样本中进行测量，并且在大多数情况下，在吸烟者中显示出异常的 BAL 值。终点包括：DNA加合物、ERK 1/2磷酸化、赖氨酰氧化酶活性、两种内源性抗氧化防御分子；中性粒细胞超氧化物释放；和 TNF mRNA。该 PI 曾处理过 BAL 样本，可以通过合作者 MD 接触吸烟者和 BAL 样本，在赖氨酰氧化酶测定方面拥有罕见的经验，并拥有相关的初步数据。该项目的积极成果可以促进对茶或茶类黄酮补充剂的研究，这些补充剂可检查癌前生物标志物（即 DNA 甲基化），并最终检查肺癌和其他癌症的发病率。阴性结果将证明重新评估如何研究绿茶的化学预防作用是合理的。绿茶可能会对身体产生有助于预防癌症的作用，但人体研究尚未完全证明这一点。该项目利用肺部的清洗样本来寻找吸烟者的这些影响，而肺部是吸烟者可能发生癌症的部位。虽然绿茶可能无法预防所有吸烟者的肺癌，但这一群体值得研究，因为他们可以比其他人更快地显示癌症相关指标的变化