Core--ANIMAL/TRANSGENIC

核心--动物/转基因

基本信息

批准号：
7458915
负责人：
GERETTE KERBY
金额：
$ 12.59万
依托单位：
BUCK INSTITUTE FOR RESEARCH ON AGING
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

项目摘要

The Animal/Transgenic Core will provide vertebrate animals and related services to Buck Institute investigators and to investigators at other institutions. General animal services will include training scientific staff in animal care and in vivo experimental procedures, providing basic vertebrate animal husbandry in an AAALAC-accredited facility, serological testing of animals for pathogens, veterinary care and consultation, necropsy services, quarantine procedures, euthanasia training and services, selected lACUC-approved surgical and nonsurgical procedures, tissue collection and storage, and transportation of animals, tissues and cells to outside investigators. Transgenic services will include production of transgenic and gene-knockout mice, breeding of genetically modified mice, genotyping, anatomical characterization of transgene expression (in collaboration with the Imaging Core), cryopreservation of transgenic mouse lines, and periodic analysis of mouse lines to ensure genetic stability.

动物/转基因核心将为Buck Institute的研究人员和其他机构的研究人员提供脊椎动物和相关服务。 General animal services will include training scientific staff in animal care and in vivo experimental procedures, providing basic vertebrate animal husbandry in an AAALAC-accredited facility, serological testing of animals for pathogens, veterinary care and consultation, necropsy services, quarantine procedures, euthanasia training and services, selected lACUC-approved surgical and nonsurgical procedures, tissue collection and storage, and transportation of animals,组织和细胞到外部研究人员。转基因服务将包括产生转基因和基因敲除小鼠，遗传改性小鼠的育种，基因分型，转基因表达的解剖表征（与成像核心合作），转基因小鼠线的冷冻保存以及对小鼠线的周期性分析以确保遗传稳定性。