THE ROLE OF THE HDL RECEPTOR, SCAVENGER RECEPTOR CLASS B TYPE I, IN HUMAN

HDL 受体（B 类清道夫受体 I 型）在人体中的作用

• 批准号: 7607491
• 负责人: Annabelle Rodriguez
• 金额: $ 0.03万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-12-01 至 2007-09-16
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7607491
• 关键词: Atherosclerosis; Cholesterol Esters; Class; Computer Retrieval of Information on Scientific Projects Database; Estradiol; Fertility; Funding; Genes; Genetic Variation; Genotype; Goals; Grant; HDL receptor; High Density Lipoproteins; Human; Infertility; Institution; Lipids; Measurement; Measures; Mediating; Numbers; Oocytes; Phenotype; Pilot Projects; Population; Pregnancy Outcome; Prevalence; RNA; Research; Research Personnel; Resources; Risk; Role; SR-BI receptor; Source; Testing; Thick; United States National Institutes of Health; Variant; Woman; granulosa cell; macrophage; monocyte; receptor; scavenger receptor; uptake

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this pilot study is to determine the lipid phenotype, SR-BI (scavenger receptor class B type I, HDL receptor) genotypes, and evidence for subclinical atherosclerosis in infertile women. These investigators have recently shown that infertile women with SR-BI RNA levels below the 25th percentile had significantly lower baseline and peak estradiol levels and lower number of retrieved and fertilized oocytes compared to women with SR-BI RNA levels above the 75th percentile. Now they seek to determine the prevalence of SR-BI variants in this population and the association of these variants with the lipid profile, subclinical atherosclerosis and fertility measurements. There are 3 specific aims: 1) To measure the lipid phenotype in infertile women and assess the ability of monocyte-derived macrophage and granulosa cells isolated from these subjects to mediate selective cholesteryl ester uptake from HDL (a functional test of SR-BI, which presumably would be impaired in low expressers compared to high expressers).; 2) To associate alterations in SR-BI expression, function and gene variants with pregnancy outcome measurements and atherosclerotic risk (assessed by intimal wall thickness on carotid doppler) in infertile women. 3) To identify variants within the SR-BI gene in infertile women.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 这项试验研究的目的是确定脂质表型，SR-BI（清道夫受体I类，HDL受体）基因型，以及不育女性亚临床动脉粥样硬化的证据。这些研究者最近表明，与高于第75％的SR-BI RNA水平相比，与第75％的女性相比，SR-BI RNA水平低于25％的不育女性的基线和峰值雌二醇水平明显降低，而被检索和受精的卵母细胞的数量较低。现在，他们试图确定该人群中SR-BI变体的普遍性，以及这些变体与脂质谱，亚临床动脉粥样硬化和生育率测量的关联。 有3个具体目的：1）测量不育女性中的脂质表型，并评估与这些受试者分离的单核细胞衍生的巨噬细胞和颗粒细胞的能力，可以介导HDL中的选择性胆固醇酯的摄取（SR-BI的功能性测试），这可能会损害高高快递的SR-BI。 2）在不育女性中，SR-BI表达，功能和基因变异的变化与妊娠结局的测量和动脉粥样硬化风险（通过颈动脉多普勒的内膜壁厚度评估）相关联。 3）鉴定不育妇女中SR-BI基因内的变体。