PACTG 1006: EFFECTS OF HAART ON THE RECOVERY OF IMMUNE FUNCTION IN HIV+ CHILDREN

PACTG 1006：HAART 对 HIV 儿童免疫功能恢复的影响

• 批准号: 7605689
• 负责人: William Borkowsky
• 金额: $ 3.73万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7605689
• 关键词: Antibody Formation; Antigens; CD4 Lymphocyte Count; CD4 Positive T Lymphocytes; Candida; Cell Count; Child; Childhood; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Growth; HIV; Hepatitis A; Hepatitis A immunization; Highly Active Antiretroviral Therapy; Immune; Immunization; Immunocompromised Host; Immunologics; Institution; Measures; Natural regeneration; Numbers; Phenotype; Plasma; Population; Purpose; RNA; Randomized; Recovery; Recovery of Function; Research; Research Personnel; Resources; Secondary Immunization; Series; Serological; Source; T-Lymphocyte; Testing; Tetanus; Tetanus Toxoid; Tetanus Vaccine; Translating; United States National Institutes of Health; Vaccination; aged; cell mediated immune response; comparative; follow-up; immune function; response; treatment duration

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This study is a randomized, comparative response study with long-term follow-up in HIV-infected children aged 2-17 years who are severely immunocompromised and are initiating highly active antiretroviral therapy (HAART). Subjects must be virologic responders, defined as >0.75 log decrease in plasma HIV RNA copy number 1 month after initiation of HAART. It will consist of approximately 90 subjects in two groups. The purpose of this study is to assess the ability of newly derived CD4 T cells to spontaneously develop lymphoproliferative responses to a recall antigen, tetanus toxoid, or to develop responses after booster vaccinations with tetanus vaccine; to assess the ability to develop protective antibody responses to a T cell-dependent antigen using a primary series of hepatitis A vaccinations.; and to measure the durability of any response beyond the last vaccination. The rationale for this study is that HIV-infected children initiating HAART have shown significant inhibition of HIV growth and significant increases in CD4 T cell counts. It is not known to what extent an increase in CD4 count in this population of children translates to a complete functional immune recovery. HIV-infected children have typically demonstrated poor serological responses to routine childhood immunizations. This study will examine the phenotype of T cells regenerated post-HAART initiation and will assess function by evaluating T cell responses to neoantigens and recall antigens. Cell-mediated immune responses to an environmental antigen (Candida), a recall antigen (tetanus), and a primary immunogen (hepatitis A) will be tested. Studies of immunologic function and recovery will be assessed and correlated with CD4 cells, HAART treatment, and duration of response.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 中心，不一定是研究者的机构。 这项研究是一项随机、比较反应研究，对 2-17 岁免疫严重受损并正在开始高效抗逆转录病毒治疗 (HAART) 的 HIV 感染儿童进行长期随访。受试者必须是病毒学应答者，定义为开始HAART后1个月血浆HIV RNA拷贝数下降>0.75 log。它将由大约 90 名受试者组成，分为两组。本研究的目的是评估新衍生的 CD4 T 细胞对记忆抗原、破伤风类毒素自发产生淋巴增殖反应的能力，或在破伤风疫苗加强接种后产生反应的能力； 评估使用主要系列甲型肝炎疫苗接种对 T 细胞依赖性抗原产生保护性抗体反应的能力。并衡量最后一次疫苗接种后任何反应的持久性。 这项研究的基本原理是，开始HAART的HIV感染儿童显示出对HIV生长的显着抑制以及CD4 T细胞计数的显着增加。目前尚不清楚该儿童群体中 CD4 计数的增加在多大程度上可以转化为功能性免疫的完全恢复。感染艾滋病毒的儿童通常对常规儿童免疫接种表现出较差的血清学反应。本研究将检查 HAART 启动后再生的 T 细胞表型，并通过评估 T 细胞对新抗原和回忆抗原的反应来评估功能。将测试针对环境抗原（念珠菌）、回忆抗原（破伤风）和主要免疫原（甲型肝炎）的细胞介导的免疫反应。将评估免疫功能和恢复的研究，并将其与 CD4 细胞、HAART 治疗和反应持续时间相关联。