Pathogen survival in transport-limited environments

病原体在运输受限的环境中存活

• 批准号: 7628062
• 负责人: AARON I PACKMAN
• 金额: $ 14.42万
• 依托单位: NORTHWESTERN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7628062
• 关键词: Address; Antibiotic Resistance; Antibiotic Therapy; Antibiotics; Antimicrobial Effect; Award; Bacteria; Biological Transport; Cell Survival; Cells; Clinical; Coupled; Coupling; Deposition; Development; Diffusion; Disease; Dose; Environment; Event; Frequencies; Gene Expression; Growth; Infection; Instruction; Knowledge; Laboratories; Liquid substance; Measures; Mechanics; Mediating; Methods; Microbial Biofilms; Microbiology; Modeling; Molecular; Mutation; Pattern; Phase; Phenotype; Play; Process; Pseudomonas aeruginosa; Research; Research Activity; Research Personnel; Research Training; Risk; Role; Stress; Structure; Survival Rate; System; Testing; Time; Training; Transport Process; Water; Work; antimicrobial; antimicrobial drug; base; career; disease transmission; experience; hazard; improved; interest; microorganism; migration; pathogen; pressure; programs; research study; solute; transmission process

DESCRIPTION (provided by applicant): The PI is a well-established junior investigator in the field of environmental fluid mechanics and transport processes, but now seeks to make a career transition to biomedical applications. The PI's interest in this field developed from preliminary work on biological transport processes in environmental systems, which led to the realization that there has been little study of the fundamental effects of physical transport on the transmission and survival of pathogenic microorgansims. In biofilm-based infections, the presence of low-transport microenvironments is expected to protect pathogens from hazards such as anti-microbial agents. Similar processes are also expected to mediate the survival of pathogens in environmental systems, and thus to play an important role in the transmission of water-borne disease. The PI proposes an extensive training and research program that will prepare him to address fundamental problems in microbiology and biomedicine while also advancing basic knowledge of the role of the physical transport environment in mediating the growth, transmission, and survival of pathogenic microorganisms. The training phase will involve coursework, clinical experience, and instruction in methods for biofilm research. Collaborative research will focus on the development of transport-limited microenvironments within biofilms and the role of these processes in mediating the transmission and survival of the representative pathogenic microorganisms P. aeruginosa and L. pneumophila in biofilms. Laboratory experiments will be used to directly observe transport rates in biofilms, determine pathogen survival when exposed to anti-microbial agents under different transport conditions, and assess the development of antibiotic resistance in pathogens residing in low-transport regions of biofilms. Quantitative analysis (modeling) will be used to assess the fundamental role of physical transport in mediating biofilm processes, and to improve analysis of the risk of water-borne disease transmission.

描述（由申请人提供）：PI是环境流体力学和运输过程领域成熟的初级调查员，但现在试图将职业过渡到生物医学应用。 PI对这一领域的兴趣来自环境系统中生物运输过程的初步工作，这导致人们意识到，几乎没有研究物理运输对致病性微角膜传播和存活的基本影响。在基于生物膜的感染中，低转移微环境的存在有望保护病原体免受抗微生物剂等危害。预计类似的过程还可以介导环境系统中病原体的存活，从而在水传播疾病的传播中发挥重要作用。 PI提出了一项广泛的培训和研究计划，该计划将使他为解决微生物学和生物医学的基本问题做好准备，同时还促进了物理运输环境在介导致病微生物的生长，传播和生存中的作用的基础知识。培训阶段将涉及生物膜研究方法的课程，临床经验和指导。协作研究将重点关注生物膜内运输受限的微环境的发展，以及这些过程在介导代表性致病性微生物的传播和存活中的作用。铜绿假单胞菌和生物膜中的肺炎。实验室实验将用于直接观察生物膜中的运输速率，确定病原体在不同运输条件下暴露于抗微生物剂时的生存率，并评估居住在生物膜低传输区域的病原体中抗生素耐药性的发展。定量分析（建模）将用于评估物理运输在介导生物膜过程中的基本作用，并改善对水传播疾病传播风险的分析。