ShEEP Request for CytoViva 3D Darkfield Hyperspectral Microscope

ShEEP 请求 CytoViva 3D 暗场高光谱显微镜

• 批准号: 9906462
• 负责人: Rajagopal Ramesh
• 金额: --
• 依托单位: OKLAHOMA CITY VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2020-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9906462
• 关键词: 3-Dimensional; Acute; Alzheimer' s Disease; Antibodies; Area; Arthritis; Award; Bacteria; Bacterial Infections; Binding; Biological; Cancer Biology; Cancer Center; Cells; Chronic Disease; Cities; Communities; Computer software; Consumption; Core Facility; Diagnosis; Disease; Drug Delivery Systems; Drug Prescriptions; Equipment; Family member; Funding; Future; Grant; Health Sciences; Healthcare Systems; Hepatitis; Image; Imagery; In Vitro; Inflammatory Bowel Diseases; Institutes; Institution; Intravenous; K-Series Research Career Programs; Label; Lead; Leadership; Legal patent; Maintenance; Malignant Neoplasms; Measurement; Mediating; Metals; Microscope; Military Personnel; Monitor; Noise; Oklahoma; Optics; Oral; Organelles; Organism; Pain; Pancreatitis; Patients; Peer Review; Pharmaceutical Preparations; Phase I Clinical Trials; Post-Traumatic Stress Disorders; Process; Protocols documentation; Quality of life; Quantum Dots; Reproduction spores; Research; Research Activity; Research Infrastructure; Research Personnel; Resources; Role; Route; Sampling; Scientist; Sheep; Ships; Signal Transduction; Small Interfering RNA; Surveys; Symptoms; System; Technology; Therapeutic; Time; Tissues; Training; Treatment Failure; Treatment outcome; United States National Institutes of Health; Universities; Veterans; Virus; Virus Diseases; Walking; Woman; animal imaging; cancer clinical trial; cancer therapy; career; clinical translation; design; drug development; drug efficacy; experience; experimental study; flexibility; human disease; improved; ineffective therapies; instrument; instrumentation; intraperitoneal; men; nanomedicine; nanoparticle; off-patent; particle; pathogen; plasmid DNA; receptor; software systems; tool; trafficking

One of the challenges in areas of drug development and drug delivery for treating various human diseases is the lack of understanding of the inherent cellular barriers that restrict the drug efficacy. Another challenge encountered by researchers conducting intracellular trafficking of molecules and their localization in cellular organelles is in the use of fluorescent labels that distort the study results with negative influence of these labels on samples. Further, lack of availability of adequate instrumentation restricts investigators from conducting cutting-edge research at their own institute. As a result they have to ship their materials to institutions/universities which are time-consuming, expensive, and do not give the investigator to know the results quickly. Therefore, continuous expansion of research infrastructure by acquiring appropriate equipment’s will enhance research activities among research investigator and lead to new discoveries. To override some of these limitations, we are requesting funds to purchase the CytoViva 3D Enhanced Darkfield Hyperspectral Microscope for enhancing the research conducted by investigators at the Oklahoma City VA Health Care System. This system offers high signal-to-noise 3D enhanced darkfield optical hyperspectral imaging specifically designed for imaging of label free particles/molecules in label-free or fluorescently labeled live cells and ex-vivo tissue matrices. The unique patented technology of the microscope allows the visualization and monitoring of particles, drugs, biologics, and cells in solution and in translucent matrices (cells, tissues, organisms). Thus, studies investigating binding of material (drug, nanoparticle, cell- particle interaction, antibody binding to cell), receptor-mediated binding and entry into cells (nanoparticles, quantum-dots, virus, bacteria, metals, antibodies) and intracellular localization and trafficking (siRNA, plasmid DNA, nanoparticles, virus, bacteria, spores) via various intracellular organelles can be determined in vitro and in ex-vivo tissue explants with label-free imaging and capture images. The system with the requisite computer software system provides quantitative spectral analysis of biological and non-biological materials imaged. The protocols can be automated giving the user to ‘walk-away’ from the instrument, and perform other experiments. Most importantly, neither the Oklahoma City VA Health Care System nor the affiliated University of Oklahoma Health Sciences Center (OUHSC) has such an instrument that enables high precision analysis of label-free materials in cells or ex-vivo tissues. Therefore, this is a truly unique and valuable resource justifying it purchase as a resource for investigators to conduct existing and new areas of research at the Oklahoma City VA Health Care System. Availability of this equipment will enhance ongoing studies and establish new areas of research benefitting the men and women serving the military and veterans. The equipment will be used by six-major users and a number of future VA users who have six VA Merit grants funded, three senior career scientist awards, and one career development award funded.

用于治疗各种人类疾病的药物开发和药物输送领域的挑战之一 缺乏对限制药物功效的固有细胞屏障的了解是另一个挑战。 研究人员进行细胞内分子运输及其在细胞中的定位时遇到的情况 细胞器使用荧光标记，这些标记的负面影响会扭曲研究结果 此外，缺乏足够的仪器限制了研究人员进行研究。 因此，他们必须将材料运送到自己​​的研究所。 机构/大学耗时、昂贵且不让研究者了解 因此，通过收购适当的研究基础设施不断扩大。 设备将加强研究人员的研究活动并带来新的发现。 为了克服其中一些限制，我们请求资金购买 CytoViva 3D 增强版 暗场高光谱显微镜用于加强俄克拉荷马州研究人员进行的研究 City VA 医疗保健系统该系统提供高信噪比 3D 增强暗场光学。 高光谱成像专为无标记或无标记颗粒/分子成像而设计 荧光标记的活细胞和离体组织基质显微镜独特的专利技术。 允许对溶液和半透明的颗粒、药物、生物制品和细胞进行可视化和监测 因此，研究研究材料（药物、纳米粒子、细胞）的结合。 颗粒相互作用、抗体与细胞结合）、受体介导的结合和进入细胞（纳米颗粒、 量子点、病毒、细菌、金属、抗体）和细胞内定位和运输（siRNA、质粒 DNA、纳米颗粒、病毒、细菌、孢子）可通过各种细胞内细胞器在体外进行测定， 在离体组织外植体中进行无标记成像并捕获图像。 软件系统提供对成像的生物和非生物材料的定量光谱分析。 协议可以自动化，让用户“离开”仪器并执行其他实验。 最重要的是，无论是俄克拉荷马城 VA 医疗保健系统还是附属大学 俄克拉荷马州健康科学中心 (OUHSC) 拥有这样一种仪器，可以实现高精度 因此，这是一种真正独特且有价值的分析。 资源证明其购买作为研究人员进行现有和新领域研究的资源是合理的 该设备的可用性将加强俄克拉荷马城退伍军人管理局医疗保健系统正在进行的研究和 建立新的研究领域，使军人和退伍军人受益。 该设备将由六大用户和一些具有六 VA 优点的未来 VA 用户使用 资助的赠款、三项高级职业科学家奖和一项职业发展奖。