NEURONAL GLIA CULTURE FACILITY

神经胶质细胞培养设施

• 批准号: 7715324
• 负责人: P. A. Ferchmin
• 金额: $ 6.83万
• 依托单位: UNIVERSIDAD CENTRAL DEL CARIBE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7715324
• 关键词: Budgets; Computer Retrieval of Information on Scientific Projects Database; Core Facility; Disease; Equipment; Fee-for-Service Plans; Funding; Goals; Grant; Image; Institution; Laboratory culture; Left; Methodology; Modeling; Neuroglia; Neurons; Preparation; Purpose; Quality Control; Research; Research Personnel; Resources; Source; System; Techniques; Time; United States National Institutes of Health; Wages; base; brain cell; experience; in vitro Model; patch clamp

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The proposed core facility facilitates the UCC investigators the use of cultured neurons, glia and organotypic cultures. The investigators no longer need to develop by themselves the techniques to culture brain cells or organotypic cultures. The cultures are used, among others, for patch clamping, Ca2+ imaging and for in vitro models of diseases. The core facility consists of a coordinator and an experience technician. The model of culture core facility proposed here is based on a similar facility in UCLA where a single technician provides glial and neuronal preparations to investigators. The proposed budget is includes salaries of the technician, consultant and the coordinator. Funds are requested for starting material and supplies, for new projects and to allow for a smooth transition to a system of fee for service. The objectives of this core facility are. a) To be abreast of new methodology in culturing and doing a constant quality control of our cultures. For that purpose we need a consultant to keep us up to date. b) To provide a faster, less expensive alternative and to discourage the initiation of new neuronal culture laboratories throughout the UCC. By fulfilling our goals we promote high quality research and increase the efficient use of space and equipment. By providing the expertise in brain cell culture and technical labor involved this core facility leaves more time to the investigators for the scientific endeavor.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 提出的核心设施促进了UCC研究人员的使用，使用了培养的神经元，神经胶质和器官型培养物。 研究人员不再需要自己开发培养脑细胞或器官培养物的技术。 除其他外，将培养物用于贴片夹，Ca2+成像和体外疾病模型。 核心设施由协调员和经验技术人员组成。 此处提出的培养核心设施的模型基于UCLA的类似设施，其中单个技术人员为研究人员提供神经胶质和神经元制剂。 拟议的预算包括技术人员，顾问和协调员的薪水。要求资金用于起始材料和耗材，新项目，并允许平稳过渡到服务费。 该核心设施的目标是。 a）在培养和对我们的文化进行持续的质量控制方面与新方法保持一致。为此，我们需要一名顾问来使我们保持最新状态。 b）提供更快，更便宜的替代方案，并劝阻整个UCC中新的神经元文化实验室的启动。 通过实现我们的目标，我们促进了高质量的研究，并提高了空间和设备的有效利用。 通过提供脑细胞培养和技术劳动方面的专业知识，该核心设施为研究人员提供了更多的时间进行科学努力。