Multi-analyte Approach for Earlier Detection of Cancers in Non Plasma Biofluids

用于早期检测非血浆生物流体中癌症的多分析物方法

• 批准号: 10763308
• 负责人: Nishant Agrawal
• 金额: $ 98.71万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-19 至 2028-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10763308
• 关键词: Achievement; Affect; Agreement; Aneuploidy; Area; Bioinformatics; Biological Assay; Biology; Blood; Brain; CLIA certified; Cancer Detection; Cerebrospinal Fluid; Cessation of life; Circulation; Clinical; Coin; Collaborations; DNA; Data; Detection; Diagnosis; Diagnostic; Early Diagnosis; Epigenetic Process; Funding; Genomic Library; Goals; Head and Neck Cancer; Head and neck structure; Individual; Industrialization; Industry Collaboration; Industry Standard; Intervention Trial; Libraries; Liquid substance; Logistic Regressions; Malignant - descriptor; Malignant Neoplasms; Malignant neoplasm of brain; Medical; Methods; Methylation; Modality; Modeling; Molecular; Morbidity - disease rate; Mutation; Neoplasms; Organ; Outcome; Output; Performance; Plasma; Procedures; Process; Protein Secretion; Protocols documentation; Quality Control; Saliva; Screening for cancer; Signal Transduction; Site; Specificity; Target Populations; Technology; Testing; Translating; Tumor-Derived; Validation; Woman; Work; brain abnormalities; brain magnetic resonance imaging; burden of illness; cancer biomarkers; cancer cell; cancer diagnosis; cancer risk; cancer type; case control; clinical application; clinical implementation; clinical translation; cohort; cost; detection assay; experience; improved; industry partner; liquid biopsy; machine learning algorithm; member; minimally invasive; mortality; neoplastic; novel; oral lesion; parent grant; prospective; sample collection; screening; specific biomarkers; standard of care; tumor; tumor DNA

PROJECT SUMMARY Early detection of cancer provides one of the most effective ways to reduce cancer related morbidity and mortality. However, most malignancies including brain and head and neck cancers do not have effective minimally invasive strategies for early detection. Unfortunately, early detection is hindered by several factors, including the lack of suitable tests and cost. One of the chief challenges in developing non-invasive tests is the identification of the appropriate biofluid and specific markers. While blood-based testing has shown promise, many cancers are not easily detected in the circulation. Over the past two decades, DNA released from cancer cells has emerged as a specific biomarker of cancer. We have previously developed sensitive methods for the simultaneous detection of multiple DNA based analytes from a small quantity of starting material in a variety of biospecimens. We have extensive preliminary results that biofluids, such as cerebrospinal fluid (CSF) and saliva, associated with certain cancers are significantly enriched in released tumor DNA (rtDNA) compared to blood. In addition, our group has an ongoing relationship with an industrial partner to translate our promising technology into the clinical realm. This proposal will build on those strengths to develop assays for the sensitive and specific detection of brain and head and neck cancer in CSF and saliva, respectively. A non-plasma based “liquid biopsy” approach for the earlier detection of cancers difficult to detect in the blood would represent a significant medical advance.

项目摘要 癌症的早期发现提供了减少癌症相关的最有效方法之一 发病率和死亡率。但是，大多数恶性肿瘤在内 癌症没有有效的最低侵入性策略来早期检测。很遗憾， 早期发现受到多种因素的阻碍，包括缺乏合适的测试和成本。一 开发非侵入性测试的主要挑战是确定适当的 生物流体和特定标记。尽管基于血液的测试已经显示出希望，但许多癌症是 在循环中不容易检测到。在过去的二十年中，DNA从癌症中释放出来 细胞已成为癌症的特定生物标志物。我们以前已经开发了敏感的 简单检测多种基于少量DNA的分析物的方法 各种生物测量中的起始材料。我们有广泛的初步结果 与某些癌症相关的生物流体，例如脑脊液（CSF）和唾液 与血液相比，与释放的肿瘤DNA（RTDNA）显着富集。此外，我们的小组 与工业合作伙伴有持续的关系，将我们的承诺技术转化为 临床领域。该提议将基于这些优势，以为 CSF和唾液中大脑和头颈癌的敏感和特异性检测， 分别。一种基于基于浮肿的“液体活检”方法，用于早期检测 很难在血液中发现将代表重大的医疗进步。

项目成果

Response rate and molecular correlates to encorafenib and binimetinib in BRAF-V600E mutant high-grade glioma.

BRAF-V600E 突变型高级别胶质瘤中恩科拉非尼和 binimetinib 的缓解率和分子相关性。

• DOI: 10.1158/1078-0432.ccr-23-3241
• 发表时间: 2024
• 期刊: Clinical cancer research : an official journal of the American Association for Cancer Research
• 作者: Schreck,KarisaC;Strowd,RoyE;Nabors,LouisB;Ellingson,BenjaminM;Chang,Michael;Tan,SzeK;Abdullaev,Zied;Turakulov,Rust;Aldape,Kenneth;Danda,Neeraja;Desideri,Serena;Fisher,Joy;Iacoboni,Michaella;Surakus,Trisha;Rudek,MichelleA
• 通讯作者: Rudek,MichelleA