Next Generation ALPHA Zebrafish Tank Washer: High Throughput with Reduced Environmental Impact

下一代 ALPHA 斑马鱼槽清洗机：高吞吐量，减少环境影响

基本信息

批准号：
10737523
负责人：
LILIANNA SOLNICAKREZEL
金额：
$ 22.32万
依托单位：
WASHINGTON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-07-01 至 2024-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10737523
关键词：
Adult Animals Beds Biomechanics Breeding Carbon Chemicals Congenital Abnormality Consumption Development Disease Dose Education and Outreach Electricity Embryo Embryo Research Ensure Environmental Impact Equipment Exhibits Faculty Filtration Fishes Food Fresh Water Genome engineering Grant Heating Hour Housing Hydrogen Peroxide Laboratories Laboratory Research Life Liquid substance Maintenance Manuals Microinjections Microscope Modeling Modernization Movement Mus Operations Research Organism Osmosis Partner in relationship Pathologic Postdoctoral Fellow Productivity Quarantine Research Research Activity Research Personnel Research Support Robotics Scientist Services Source Steam System Technology Testing Time United States National Institutes of Health Universities Walking Washington Water Water Purification Zebrafish feeding fine particles genetic analysis graduate student human model improved manufacture medical schools member next generation operation outreach program particle programs prototype repaired sensor sperm cryopreservation ultraviolet irradiation undergraduate student water flow water quality zygote

Adult Animals Beds Biomechanics Breeding Carbon Chemicals Congenital Abnormality Consumption Development Disease Dose Education and Outreach Electricity Embryo Embryo Research Ensure Environmental Impact Equipment Exhibits Faculty Filtration Fishes Food Fresh Water Genome engineering Grant Heating Hour Housing Hydrogen Peroxide Laboratories Laboratory Research Life Liquid substance Maintenance Manuals Microinjections Microscope Modeling Modernization Movement Mus Operations Research Organism Osmosis Partner in relationship Pathologic Postdoctoral Fellow Productivity Quarantine Research Research Activity Research Personnel Research Support Robotics Scientist Services Source Steam System Technology Testing Time United States National Institutes of Health Universities Walking Washington Water Water Purification Zebrafish feeding fine particles genetic analysis graduate student human model improved manufacture medical schools member next generation operation outreach program particle programs prototype repaired sensor sperm cryopreservation ultraviolet irradiation undergraduate student water flow water quality zygote

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项目摘要

Zebrafish is a premier research organism for the genetic analysis of vertebrate development and modeling human birth defects and disease. The Zebrafish Facility at Washington University School of Medicine in St. Louis is a shared-used facility. This 5,644 sf state-of-the-art facility accommodates a total of nearly 9,000 tanks in four animal holding rooms with independent recirculating water systems. The Facility provides optimal conditions for the rearing and maintenance of up to 200,000 fish. Starting with four zebrafish laboratories in 2011, the Facility currently supports research of 33 independent laboratories. Presently, over 100 graduate and undergraduate students, postdoctoral fellows and scientists carry out research in the Facility that is supported by R00, R01, R54, and P01 grants from the National Institutes of Health. The Facility also supports other educational and outreach programs. The Facility enables these programs by ensuring superior husbandry with automatic feeding that halves the time zebrafish grow to adulthood; supplying wild-type lines and embryos for experimentation, cryopreserving sperm to minimize the number of lines maintained as life stocks, conducting pathological testing; maintaining Quarantine System for imported lines, providing research services such as genome engineering, and washing and disinfecting holding and breeding tanks and their components to ensure safe and efficient research operations. The continued increase of the zebrafish users and expansion to 9,000 tanks requires modern, technologically advanced equipment to clean and disinfect, on a daily basis, hundreds of holding and breeding tanks, and tank components as well as fish nets to ensure safe and efficient zebrafish research. Here we propose replacing the existing 900 GP Walk in Washer (Tecniplast) with the next generation ALPHA Walk in Washer model (Tecniplast). The existing 900 GP Washer is a prototype aquatics washer with several limitations: it uses steam lines to heat water, is energetically inefficient, requires long wash cycles, uses an external chemical dosing system, and breaks down frequently. These inefficiencies and frequent downtimes increase manual labor, limiting and slowing down the Facility operations and research activities it supports. The ALPHA model deploys adaptive cleaning technology that combines independent vertical and oscillating movements allowing for superior water coverage. The ALPHA washer uses electricity to heat water, shorter wash cycles and thus higher throughput that would meet the continually growing washing needs of the zebrafish researchers. The reservoir for the chemicals used during wash cycles fit into the machine footprint. Operations with the ALPHA Washer would reduce environmental impact with a shorter wash cycle and consuming smaller amounts of chemicals per cycle. The proposed upgrade to the modern ALPHA Walk in Washer would dramatically improve the washing capacity of zebrafish holding and breeding tanks; would meet the needs of the growing zebrafish research operations and would also reduce the Facility’s environmental impact.

斑马鱼是脊椎动物发育和建模的遗传分析的主要研究生物人类的先天缺陷和疾病。华盛顿大学医学院圣路易斯的斑马鱼设施是共享的设施。这个5,644平方英尺的最先进的设施可容纳四个坦克具有独立循环供水系统的动物持有房间。该设施为饲养和维护多达200,000条鱼。从2011年的四个斑马鱼实验室开始，该设施目前支持33个独立实验室的研究。目前，超过100个毕业生和本科学生，博士后研究员和科学家在R00，R01支持的设施中进行研究 R54和美国国立卫生研究院的P01赠款。该设施还支持其他教育，外展计划。该设施通过确保自动喂养来确保出色的畜牧业来实现这些程序斑马鱼成年的时间一半。提供野生型线和胚胎进行实验，冷冻水平的精子以最大程度地减少维持生命库存的线的数量，从而进行病理测试；维护进口线的隔离系统，提供基因组工程等研究服务，以及洗涤和消毒储罐及其组件，以确保安全有效研究行动。斑马鱼使用者的持续增加，并扩展到9,000辆坦克需要现代，从技术上讲每天清洁和消毒的先进设备，数百个持有和繁殖坦克和坦克组件和鱼网，以确保安全有效的斑马鱼研究。在这里，我们建议取代在Washer（Tecniplast）的现有900 GP步行（tecniplast）。现有的900 GP洗衣机是一个原型水产品洗衣机，有几个限制：它使用Steam 加热水的线本质上是低效的，需要长的洗涤周期，使用外部化学剂量系统，并经常分解。这些效率低下和经常下降时增加了体力劳动，限制并减慢其支持的设施操作和研究活动。 Alpha模型部署了自适应清洁技术，该技术结合了独立的垂直和振荡动作允许优越的水覆盖范围。 Alpha洗衣机使用电加热水，短洗涤周期，从而更高的吞吐量，可以满足斑马鱼的不断增长的洗涤需求研究人员。洗涤循环期间使用的化学物质的水库适合机器足迹。运营使用Alpha洗衣机将随着较短的洗涤周期而减少环境影响，并且消耗较小每个周期的化学物质量。拟议的升级到Washer的现代Alpha Walk Will 龙起来提高斑马鱼的洗涤能力和繁殖罐的洗涤能力；会满足发展斑马鱼研究行动，还将减少该设施的环境影响。