Low Cost Multi-pathogen Laser Diagnostic for HIV and AIDS Co-Infections

HIV 和 AIDS 合并感染的低成本多病原体激光诊断

基本信息

批准号：
7460723
负责人：
Christopher John Myatt
金额：
$ 33.11万
依托单位：
PRECISION PHOTONICS CORPORATION (PPC)
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-01-15 至 2009-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7460723
关键词：
AIDS clinical trial group Acquired Immunodeficiency Syndrome Address Anti-Retroviral Agents Antibodies Antigens Architecture Automobile Driving Award Biological Assay Blood Blood Screening Blood Transfusion Blood-Borne Pathogens Brazil California Caring Class Clinic Clinical Clinical Investigator Clinical Microbiology Clinical Trials Consumption Cytomegalovirus Data Detection Developed Countries Developing Countries Devices Diagnosis Diagnostic Differential Diagnosis Disease Electronics Goals Government Agencies HIV HIV diagnosis HIV-1 Head Heart Hepatitis B Virus Hepatitis C virus Hospitals Human Herpesvirus 8 Image Immunoassay Individual Infection Institutes Ions Laboratories Lasers Legal patent Licensing Life Lighting Measurement Medical Methodology Methods Nobel Prize Numbers Optics Patients Performance Persons Phase Physical condensation Physicians Population Price Range Reader Reading Reagent Research Research Infrastructure Research Personnel Resources Risk Safety Sampling Screening procedure Serological Simplexvirus Spottings Standards of Weights and Measures System Techniques Technology Testing Time Today Toxoplasma gondii Tracer Universities Work base burden of illness cohort cost cost efficient design experience health care quality improved innovation novel pathogen photonics point of care sensor skills stem transmission process

项目摘要

DESCRIPTION (provided by applicant): Precision Photonics Corporation proposes to develop and demonstrate the key technologies for a laser based multi-pathogen diagnostic system for HIV and common co-infections. The ultimate goal is to provide very low cost, easy to use tests at the point of care to help diagnose HIV and its co-infections in resource limited settings. The technology will use novel diode laser illumination and ultra-sensitive detection techniques with low cost optics and lasers, analogous to a DVD sensor head. By combining sensitive detection with an immunoassay format, reagent consumption is minimized. An array of detection spots and segregated tracers allow multi-analyte detection for multi-pathogen diagnosis. In the proposed project, we will (i) Establish a working, manufacturable reference design of the fluidic assay components and fluorescent reader. The cost/performance goals are to achieve a design that can meet a manufacturing cost of <US$50 (goal of US$20) for the reader, <US$0.5 (goal of $0.25) for the cartridge, and with adequate sensitivity for serological assays, (ii) Develop an assay panel for detection of antibodies to HIV, HCV, HBV, HHV-8, HSV 1 & 2, CMV, and T. gondii, (iii) Test the assay panel at UCSD in a cohort of HIV-1 infected persons, and (iv) Test the assay abroad as time and resources permit. The research performed in this project, if successful, will enable very low cost, easy to use tests at the point of care to help in assessment of disease status of AIDS patients in resource limited settings. The simplicity and low cost of the technology will facilitate the use of anti-retroviral therapy, greatly prolonging the lives of those infected with HIV and reducing the burden of disease in populations where HIV is endemic.

描述（由申请人提供）：精确光子公司建议开发和演示基于激光的艾滋病毒和常见共同感染的基于激光的多病原诊断系统的关键技术。最终目标是在护理点上提供非常低的成本，易于使用的测试，以帮助诊断艾滋病毒及其在资源有限设置中的共同感染。该技术将使用新型的二极管激光照明和具有低成本光学和激光器的超敏感检测技术，类似于DVD传感器头。通过将敏感检测与免疫测定格式相结合，可以最大程度地减少试剂的消耗。一系列检测点和隔离的示踪剂允许多种病原体诊断多分析物检测。在拟议的项目中，我们将（i）建立流体测定组件和荧光读取器的可行，可制造的参考设计。成本/绩效目标是实现可以满足读者的制造成本<50美元（目标20美元）的设计，<0.5美元（目标为0.25美元），并且具有足够的血清学测定敏感性，（ii）开发一个用于检测HIV，HCV，HCV，HBV，HBV，HHV，HHV，HHV，HHV，HHV，HHV，HHV，HHV，HHV，HHV，HHV，HHV，HHV，HHV，HHV，HHV，HHV，HHV，HHV，HHV，HHV，HHV，HHV，HHV，HHV，HHV，HHV，HHV，HHV-8，和Gondii，（iii）在一组HIV-1感染者中测试UCSD的测定面板，并（iv）在国外测定在国外的时间和资源许可证。如果成功的话，在该项目中进行的研究将使非常低成本，在护理点易于使用，以帮助评估资源有限的环境中艾滋病患者的疾病状况。该技术的简单性和低成本将有助于使用抗逆转录病毒疗法，从而大大延长感染HIV感染者的生活，并减轻了艾滋病毒疾病的疾病负担。