Enrichment-free Salmonella detection for food safety

用于食品安全的无富集沙门氏菌检测

• 批准号: 9409755
• 负责人: Michael Sandor Koeris
• 金额: $ 48.95万
• 依托单位: SAMPLE6 TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-07-12 至 2017-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9409755
• 关键词: Alfalfa; American; Bacterial Infections; Bacteriophages; Biological Assay; Categories; Cells; Centers for Disease Control and Prevention (U.S.); Cessation of life; Consumption; Detection; Development; Diagnostic; Disease; Disease Outbreaks; Economic Burden; Engineering; Enzymes; Etiology; Food; Food Contamination; Food Safety; Grant; Guidelines; Hospitalization; Individual; Industrialization; Infection; Infection prevention; International; Laboratories; Link; Listeria; Manufacturer Name; Measures; Meat; Methods; Phase; Pistachio Nuts; Plants; Process; Production; Public Health; Reporting; Salmonella; Salmonella infections; Serotyping; Signal Transduction; Small Business Innovation Research Grant; Specificity; Surface; System; Technology; Testing; Time; United States; United States National Institutes of Health; Validation; Vegetables; Work; assay development; base; clinically relevant; cost; disorder prevention; egg; foodborne; foodborne illness; gastrointestinal; health care economics; new technology; novel; novel diagnostics; pathogen; surveillance network; undercooked

Project Summary/Abstract Each year over one million people become ill due to foodborne Salmonella infections, this accounts for a total of 11% of all foodborne illness reported in the United States1. Salmonella is the leading cause for foodborne illness hospitalizations (23,000 cases; 35%) and deaths (450 cases; 28%). Salmonella has an enormous impact on the economy, causing $3.7 billion in costs in the United States each year; 90% of this burden ($3.3 million) is due to deaths, 8% ($294 million) due to hospitalizations and 2% due to non-hospitalized cases. The primary mode of Salmonella infection is through food, mainly raw or undercooked meat, raw eggs and unwashed vegetables. These illnesses are often tied to outbreaks that result from contaminated food that was not detected at a manufacturing or packaging plant. Current detection methods for Salmonella provide slow time to actionable results, making it difficult to take necessary steps in controlling the pathogen. Sample6 has developed a new technology, based on the proprietary Bioillumination PlatformTM, which utilizes a novel synthetic engineering approach to leverage the natural specificity of bacteriophage to detect the presence of specific bacterial species. Sample6 DETECT/S system contains engineered bacteriophages, which can identify Salmonella spp. to induce production of luminescent enzymes, upon infection of the target cells, effectively illuminating Salmonella. This signal can be measured with the Sample6 Luminometer and instantiates the world's first enrichment-free pathogen diagnostic system. Sample6 has previously established feasibility that the DETECT/S assay can be adapted for finished product testing to detect Salmonella directly on food products that are prone to contamination. For this Direct-to- Phase II project, we propose to complete the development of our technology to detect Salmonella on additional foods to achieve validation as laid out in AOAC guidelines. We will expand the testing to include 400 Salmonella isolates belonging to clinically relevant serotypes, and complete assay development for 20 food matrices representing all commercially relevant categories, including low-moisture foods. Finally, we will develop non-replicating phage technology for our BioIllumination Platform to further increase our reach in the market, both domestically and internationally.

项目概要/摘要 每年有超过一百万人因食源性沙门氏菌感染而患病，这 占美国报告的所有食源性疾病的 11%1。沙门氏菌是 食源性疾病住院（23,000 例；35%）和死亡（450 例）的主要原因 案例； 28%）。沙门氏菌对经济造成巨大影响，造成 37 亿美元的损失 每年在美国；该负担的 90%（330 万美元）是由于死亡，8%（294 美元） 百万）由于住院病例和 2% 由于非住院病例。主要模式为 沙门氏菌是通过食物感染的，主要是生的或未煮熟的肉、生鸡蛋和未洗的肉 蔬菜。这些疾病通常与受污染食品引起的疾病爆发有关。 未在制造或包装工厂检测到。目前的检测方法 沙门氏菌导致获得可行结果的时间很慢，因此很难采取必要的措施 控制病原体。 Sample6 开发了一项新技术，基于专有技术 生物照明平台TM，利用新颖的合成工程方法来利用 噬菌体的天然特异性可检测特定细菌种类的存在。样品6 DETECT/S 系统包含工程噬菌体，可以识别沙门氏菌属。到 在感染靶细胞后，有效诱导发光酶的产生 阐明沙门氏菌。该信号可以使用 Sample6 发光计进行测量， 实例化了世界上第一个无浓缩病原体诊断系统。 Sample6 之前有 确定 DETECT/S 测定可适用于成品测试的可行性 直接检测易受污染的食品上的沙门氏菌。对于这个直接到 二期项目，我们建议完成沙门氏菌检测技术的开发 AOAC 指南中规定的其他食品以实现验证。我们将扩大 测试包括 400 个属于临床相关血清型的沙门氏菌分离株，并完成 代表所有商业相关类别的 20 种食品基质的测定开发， 包括低水分食物。最后，我们将开发非复制噬菌体技术 生物照明平台进一步扩大我们在国内外市场的影响力 国际上。