Transcriptomes and Proteomes of Plasmodium Vivax

间日疟原虫的转录组和蛋白质组

基本信息

批准号：
9241966
负责人：
LIWANG CUI
金额：
$ 16.97万
依托单位：
PENNSYLVANIA STATE UNIVERSITY, THE
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-03-15 至 2018-09-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9241966
关键词：
Achievement Address Affect Area Benign Biological Biology Blood Case Management Central Asia Chloroquine resistance Clinical Clinical Management Clinical Pathology Communities Coupled Culicidae Culture Techniques DNA Microarray Chip Data Development Developmental Biology Disease Epidemiology Erythrocytes Gene Expression Genome Genomics Geography Hepatocyte Hour Human In Vitro Interruption Invaded Kinetics Knowledge Lead Liver Malaria Mass Spectrum Analysis Measures Methods Monkeys Morbidity - disease rate Parasites Pharmaceutical Preparations Plasmodium Plasmodium falciparum Plasmodium vivax Play Population Post-Transcriptional Regulation Preparation Prevalence Primaquine Production Proteins Proteome Proteomics Publishing Recurrent disease Research Resources Reticulocytes Role Sampling Sexual Development Southeastern Asia Sporozoites Surface Antigens Symptoms System Systems Biology Techniques Technology Time Vivax Malaria Work analytical tool asexual base comparative comparative genomics drug discovery gene product humanized mouse improved innovation liver development malaria transmission mortality mouse model neglect novel therapeutics novel vaccines parasite genome public health relevance transcriptome transcriptome sequencing transcriptomics transmission process transmission-blocking vaccine trend vaccine development vaccine discovery

项目摘要

DESCRIPTION (provided by applicant): Plasmodium vivax is the second most prevalent and the most geographically widespread malaria parasite, causing enormous morbidity and mortality in affected populations. Research on P. vivax malaria has lagged significantly behind, largely due to the lack of a continuous in vitro culture system. This parasite selectively invades reticulocytes, making long-term culture difficult. P. vivax gametocytes, which are responsible for transmission, are produced before the onset of the clinical symptoms. Though the first P. vivax genome was published in 2008, systematic comparison of the transcriptomes and proteomes of the asexual erythrocytic cycle is lacking and we know little about the developmental biology of P. vivax gametocytes. This project, built on recent advancement in humanized mouse model to study the liver stages of this parasite and our improved in vitro culture techniques for synchronous blood stages, aims to determine the temporal transcriptomes and proteomes during P. vivax asexual erythrocytic development and gametocytogenesis using RNA sequencing and mass spectrometry technologies, respectively. The comprehensive transcriptomic and proteomic data generated from this study will enable systems biology approaches to address the fundamental biology of asexual and sexual development of this neglected malaria parasite. This information is also deemed critical for the discovery of novel drugs and vaccines for the interruption of P. vivax transmission.

描述（由申请人提供）：间日疟原虫是第二大流行且地理分布最广的疟疾寄生虫，在受影响人群中造成巨大的发病率和死亡率，对间日疟原虫的研究明显滞后，这主要是由于缺乏持续的研究。这种寄生虫选择性地侵入网织红细胞，导致在临床症状出现之前产生负责传播的间日疟原虫配子细胞。第一个间日疟原虫基因组于 2008 年发表，缺乏对无性红细胞周期的转录组和蛋白质组的系统比较，而且我们对间日疟原虫配子体的发育生物学知之甚少。该项目建立在人源化小鼠模型的最新进展之上。研究这种寄生虫的肝脏阶段以及我们改进的同步血液阶段体外培养技术，旨在确定间日疟原虫无性红细胞过程中的时间转录组和蛋白质组分别使用RNA测序和质谱技术进行发育和配子细胞发生，这项研究产生的全面转录组和蛋白质组数据将使系统生物学方法能够解决这种被忽视的疟原虫无性和有性发育的基本生物学问题。发现用于阻断间日疟原虫传播的新药物和疫苗。