An upright multiphoton microscope for biomedical research applications

用于生物医学研究应用的正置多光子显微镜

• 批准号: 9274589
• 负责人: DANIEL A LLANO
• 金额: $ 77.05万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-03-01 至 2019-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9274589
• 关键词: Basic Science; Biological; Biomedical Research; Cognition Disorders; Discipline; Doctor of Philosophy; Electrophysiology (science); Ensure; Funding; Illinois; In Vitro; Inherited; Institutes; Invertebrates; Lasers; Microscope; Microscopy; Minor; National Institute of Allergy and Infectious Disease; National Institute of Biomedical Imaging and Bioengineering; National Institute of Drug Abuse; National Institute of General Medical Sciences; National Institute of Mental Health; National Institute of Neurological Disorders and Stroke; National Institute on Deafness and Other Communication Disorders; Nature; Neurobiology; Neurons; Research; Research Infrastructure; Research Personnel; Research Support; Resource Sharing; Science; System; Technology; Time; Tissue imaging; Tissues; Translational Research; United States National Institutes of Health; Universities; base; cancer diagnosis; experience; in vivo; instrument; interest; new technology; optogenetics

Project Summary In this proposal, we request funding for the purchase of a multiphoton microscope system to be housed in the Beckman Institute for Advanced Science and Technology at the University of Illinois. The state-of-the-art microscopy system will permit deep-tissue imaging into fixed and live tissues and serve as a shared resource for multiple investigators at the University of Illinois. The microscope will be outfitted with an extended infrared laser, optogenetic stimulation capability and an easily moveable stage to permit in vitro as well as in vivo electrophysiology experimentation. In addition, the microscope will be supported and managed by the Beckman Institute Microscopy Suite, for which there is already dedicated space and full-time Ph.D.-level staff to manage the instrument. The microscopy suite is a unit that is fully supported by the Beckman Institute, with paid staff who have extensive experience in managing high-end laser- based microscopy systems. The Microscopy Suite also has infrastructure to ensure maximal utilization of the microscope by NIH-funded users. Eleven major users and one minor user have been identified, and their diverse interests represent the interdisciplinary nature of the Beckman Institute. The major users come from 8 different departments and use of a multiphoton microscope will support their NIH-supported research, with funding coming from 8 different institutes (NCI, NIAID, NIBIB, NIDA, NIDCD, NIMH, NIGMS and NINDS). The major users have research interests in developing novel technology platforms for cancer diagnosis, for understanding the biological underpinnings of inherited cognitive disorders, for understanding invertebrate neurobiology, studying glial-neuronal interactions and others. Therefore, the multiphoton microscope would support both basic and translational research across a variety of disciplines, and support ongoing NIH-funded research at Illinois.

项目概要 在此提案中，我们请求资助购买多光子显微镜系统 位于英国大学贝克曼先进科学技术研究所 伊利诺伊州。最先进的显微镜系统将允许对固定和固定的深层组织进行成像 活组织并作为伊利诺伊大学多个研究人员的共享资源。 该显微镜将配备扩展的红外激光、光遗传学刺激 能力和易于移动的阶段，以允许体外以及体内电生理学 实验。此外，该显微镜将由贝克曼公司提供支持和管理 研究所显微镜套件，已有专用空间和全职博士级人员 人员来管理仪器。显微镜套件是一个得到完全支持的单元 贝克曼研究所拥有在管理高端激光方面拥有丰富经验的受薪员工 基于显微系统。显微镜套件还拥有基础设施，以确保最大程度地 NIH 资助的用户对显微镜的使用。 11 个主要用户和 1 个次要用户 已被确定，他们的不同兴趣代表了贝克曼的跨学科性质 研究所。主要用户来自8个不同的部门并且使用多光子 显微镜将支持他们由 NIH 支持的研究，资金来自 8 个不同的领域 机构（NCI、NIAID、NIBIB、NIDA、NIDCD、NIMH、NIGMS 和 NINDS）。主要用户有 研究兴趣是开发癌症诊断的新技术平台， 了解遗传性认知障碍的生物学基础，以便理解 无脊椎动物神经生物学，研究神经胶质-神经元相互作用等。因此， 多光子显微镜将支持各种基础研究和转化研究 学科，并支持伊利诺伊州正在进行的 NIH 资助的研究。