Oxidative Stress Biosensor for Breath-Based Environmental Monitoring

用于基于呼吸的环境监测的氧化应激生物传感器

• 批准号: 9408215
• 负责人: Ray Anthony Martino
• 金额: $ 14.99万
• 依托单位: ISENSE, LLC
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-15 至 2018-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9408215
• 关键词: Acetone; Acute; Air Pollution; Aldehydes; Algorithms; Ammonia; Antioxidants; Area; Benchmarking; Biochemical; Biochemical Process; Biological Markers; Biosensor; Breath Tests; Cancer Patient; Carbon Monoxide; Cardiovascular Diseases; Chemicals; Chemistry; Chronic Disease; Classification; Clinic; Complex Mixtures; Data Analyses; Detection; Development; Devices; Disease; Distress; Endocrine Disruptors; Environmental Exposure; Environmental Monitoring; Evaluation; Exhalation; Failure; Fingerprint; Formulation; Health; Health Status; Heavy Metals; Human; Human Resources; Hydrocarbons; Hydrogen Peroxide; Hydrogen Sulfide; Individual; Industrialization; Inflammatory Response; Isoprene; Kidney Diseases; Libraries; Malignant neoplasm of lung; Malondialdehyde; Measurement; Measures; Methods; Monitor; Neurodegenerative Disorders; Nitric Oxide; Nitrogen Oxides; Noise; Organ; Oxidative Stress; Pathogenesis; Pathway interactions; Patient Care; Patients; Performance; Peroxides; Pesticides; Phase; Physical Examination; Physicians; Physiological; Production; Public Health; Reactive Oxygen Species; Reporting; Reproducibility; Research; Screening Result; Sensitivity and Specificity; Signal Transduction; System; Technology; Testing; Time; Toxic Environmental Substances; Vulnerable Populations; Work; Xenobiotics; base; biomarker identification; biosignature; carcinogenesis; chemical fingerprinting; environmental stressor; hexanoic acid; improved; insight; interest; n-hexanal; novel; optical sensor; oxidation; oxidative damage; portability; repaired; response; sensor; success; tool; urban area

PROJECT SUMMARY/ABSTRACT This application seeks to develop a non-invasive and practical optical sensor technology to monitor and quantify oxidative health. Oxidative stress (OS) is a biochemical imbalance in which production of reactive oxygen species exceeds the system’s antioxidant capacity. Failure to repair or contain cumulative oxidative damage is likely responsible for the pathogenesis of many acute and chronic illnesses, including cardiovascular and kidney disease, inflammatory responses, neurodegenerative disorders, and carcinogenesis. A noninvasive, inexpensive, robust sensor capable of evaluating and monitoring systemic oxidative stress would be a tool of great value to public health officials, clinicians, and individual consumers since many environmental conditions and xenobiotics (e.g., urban air pollution, heavy metal contamination, endocrine disrupting compounds, pesticides, and industrial chemicals) trigger oxidative stress. Because there is no universal biomarker for OS and there are many potential target molecules, it’s critical to develop a system that is capable of detecting many chemical components and creating a chemical fingerprint that reflects alterations in key pathways associated with environmental stressors. iSense, LLC has developed a novel sensor array that can detect a vast library of volatile compounds across multiple chemical moieties at ppb-level concentrations. The technology is rapid, inexpensive, and capable of distinguishing between single analytes and complex mixtures. Our breath sensor has been used to detect the biosignature of lung cancer patients and differentiate between different types of lung cancer in humans. Phase I of this project will test, improve, and optimize this breath analysis technology towards the detection and identification of key biomarkers of oxidative stress. Successful completion of this work will establish the fundamental chemistry necessary to develop a device to measure and understand markers of environmental exposure.

项目概要/摘要 该应用旨在开发一种非侵入性实用的光学传感器技术来监测和 量化氧化应激 (OS) 是一种生化失衡，其中会产生反应性物质。 氧气超过系统的抗氧化能力，无法修复或抑制累积的氧化。 损害可能是许多急性和慢性疾病的发病机制，包括心血管疾病 以及肾脏疾病、炎症反应、神经退行性疾病和致癌作用。 能够评估和监测全身氧化应激的廉价、坚固的传感器将成为一种工具 对于公共卫生官员、喜爱者和个人消费者来说具有巨大的价值，因为许多环境因素 条件和外源物质（例如城市空气污染、重金属污染、内分泌干扰物） 化合物、农药和工业化学品）会引发氧化应激，因为没有通用的方法。 OS 的生物标志物，并且有许多潜在的目标分子，开发一个能够实现这一目标的系统至关重要 检测许多化学成分并创建反映关键变化的化学指纹 iSense, LLC 开发了一种新型传感器阵列，可以 检测 ppb 级浓度的多个化学部分的大量挥发性化合物。 该技术快速、廉价，并且能够区分单一分析物和复杂混合物。 我们的呼吸传感器已用于检测肺癌患者的生物特征并区分 该项目的第一阶段将测试、改善和优化这种呼吸。 检测和鉴定氧化应激关键生物标志物的分析技术成功。 这项工作的完成将建立开发测量和测量设备所需的基本化学原理。 了解环境暴露的标志。