Animal Care Core

动物护理核心

基本信息

批准号：
9325003
负责人：
Kristin Nicole Grimsrud
金额：
$ 17.37万
依托单位：
UNIVERSITY OF CALIFORNIA AT DAVIS
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-07-01 至 2021-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9325003
关键词：
Acclimatization Age Animal Housing Animal Model Animals Area Bariatrics Behavior Behavioral Biology Blood specimen Caring Communicable Diseases Containment Cryopreservation Data Data Collection Endocrinology Enrollment Ensure Environment Euthanasia Evaluation Exercise Female Gastrectomy Gastric Bypass Gender Gene Targeting Generations Genetic Genetic Engineering Genetically Engineered Mouse Glucose tolerance test Gnotobiotic Health Health Services Health Status Housing Human Resources IACUC In Vitro Indwelling Catheter Infusion procedures Institution Laboratories Laboratory Animals Maintenance Metabolic Microinjections Microsurgery Mission Modeling Monitor Mus Mutant Strains Mice Operative Surgical Procedures Phenotype Procedures Quarantine Reproducibility Research Research Infrastructure Research Personnel Resources Rodent Sampling Schedule Services Shipping Ships Specialist Students Surgical Models Telemetry Testing Time Training Vendor Veterinarians Work animal care animation authority bariatric surgery cognitive function cohort cost effective environmental enrichment for laboratory animals experience feeding genome editing gut microbiome implantation in vivo insulin tolerance laboratory experience laboratory facility male meetings metabolic phenotype microbiome mouse model mutant mouse model programs repository sample collection

项目摘要

CORE B: ANIMAL CARE CORE ABSTRACT (CORE B) The Animal Care Core (Core B) provides project support for animal care and housing, importation, in vivo procedural sampling, and animal model creation for the MMPC-UCD. The Core accomplishes this through importation and acclimation of mice with minimal delay and reduced quarantine. The Core has access to and oversight of a dedicated in vivo Phenotyping Facility and associated state-of-the-art vivaria, including a high- containment “approved vendor-status” barrier vivarium. In addition, the Core has primary access to a new, UCD Gnotobiotic Mouse Research Center (GMRC). The Core provides numerous scientific support services as well, including rederivation and creation of genetically-modified mutant mice, re-animation of a variety of models cryopreserved within the KOMP or MMRRC repositories. These new and extant mouse models can then be bred to produce male and female cohorts for in vivo and in vitro phenotyping, including rederivation into the GMRC for gut microbiome studies. The Core also operates a Microsurgery Suite for creating surgically-manipulated mouse models, such as bariatric surgical models. The Core can produce for researchers cohorts of mice that have undergone Vertical Sleeve Gastrectomy (VSG) and Roux en Y Gastric Bypass (RYGB) bariatric procedures. Upon request from users, the Core can work with the other Phenotyping Cores to provide testing, manipulation, and sampling of these models. In addition, the Core can ship these surgically-manipulated models as well as any genetically-engineered mouse lines to requesting investigators from our approved vendor barrier vivarium, facilitating direct entry into the recipient institution's vivarium. This ensures minimal delay for investigators for receipt and use of these animal models at their institution. The Core also provides ancillary services and procedures, including blood sampling for in vivo testing for glucose tolerance tests and insulin tolerance tests, which are then sent to the Phenotyping Cores (e.g., Core C, Endocrinology and Metabolism Core) for analysis. The Core can also provide telemetry implantation and indwelling catheter placement for continuous blood sampling. The Core is supported by two trained laboratory animal veterinarians, three trained rodent surgical technicians, one import/export coordinator, and two phenotyping technicians. In these ways, the Animal Care Core is uniquely qualified to support the varied in vivo needs of MMPC-UCD users to meet their specific needs, from rapid importation and testing, genetically- engineered and genome-edited (Cas9) mouse lines, bariatric and other surgically-manipulated models, specialized housing under barrier conditions for direct shipping to investigators, and maintenance under gnotobiotic conditions for microbiome manipulation.

核心 B：动物护理核心摘要（核心 B）动物护理核心（核心 B）为动物护理和饲养、进口、体内 The Core 通过程序采样和动物模型创建来实现这一目标。核心可以以最短的延迟和减少检疫来进口和驯化小鼠。监督专门的体内表型分析设施和相关的最先进的vivaria，包括高此外，核心还可以主要访问一个新的、具有“批准的供应商状态”的屏障饲养室。都柏林大学知生小鼠研究中心 (GMRC) 提供众多科学支持服务。以及，包括重新衍生和创造转基因突变小鼠，重新激活各种冷冻保存在 KOMP 或 MMRRC 存储库中的模型这些新的和现有的小鼠模型可以。然后进行繁殖，产生雄性和雌性群体，用于体内和体外表型分析，包括再衍生进入 GMRC 进行肠道微生物组研究，The Core 还运营一个显微外科套件来创建。 The Core 可以生产手术操作的小鼠模型，例如减肥手术模型。研究人员对接受了垂直袖状胃切除术 (VSG) 和 Roux en Y 胃切除术的小鼠进行了分组根据用户的要求，绕过（RYGB）减肥程序，核心可以与其他表型分析一起使用。提供这些模型的测试、操作和采样的核心此外，核心还可以运送这些模型。向有要求的研究人员提供手术操作模型以及任何基因工程小鼠品系来自我们批准的供应商屏障动物园，方便直接进入接收机构的动物园。确保研究人员在其机构接收和使用这些动物模型时的延误最小化。 Core 还提供辅助服务和程序，包括用于体内葡萄糖检测的血液采样耐受性测试和胰岛素耐受性测试，然后发送到表型分析核心（例如核心 C、内分泌和代谢核心）用于分析，该核心还可以提供遥测植入和用于连续血液采样的留置导管放置由两个训练有素的实验室提供支持。动物兽医、三名经过培训的啮齿动物外科技术人员、一名进出口协调员和两名通过这些方式，动物护理核心具有独特的资格来支持各种不同的情况。 vivo需要MMPC-UCD用户来满足他们的特定需求，从快速导入和测试，基因- 工程和基因组编辑 (Cas9) 小鼠品系、减肥模型和其他手术操作模型，障碍条件下的专门住房，可直接运送给调查人员，并在用于微生物组操纵的无菌条件。